Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production
Aspergillus flavus produces a lipase (EC 3.1.1.3) which is partly bound to the mycelium during growth. The production of the mycelium-bound lipase is concomitant with growth, and declines when growth ceases. Maximum productivity of the enzyme is obtained when the culture is incubated at 30°C, an ini...
| _version_ | 1848849974234710016 |
|---|---|
| author | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Ampon, Kamaruzaman Bucke, Christopher |
| author_facet | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Ampon, Kamaruzaman Bucke, Christopher |
| author_sort | Long, Kamariah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aspergillus flavus produces a lipase (EC 3.1.1.3) which is partly bound to the mycelium during growth. The production of the mycelium-bound lipase is concomitant with growth, and declines when growth ceases. Maximum productivity of the enzyme is obtained when the culture is incubated at 30°C, an initial culture pH of 6·5 and with 2% (w/v) each of corn oil and yeast extract as carbon and organic nitrogen source. Yeast extract affects not only the production of lipase but also the secretion of proteases into the culture medium. Production of the latter enzymes, which inactivate the free lipase, is enhanced by adding yeast extract (1–2%, w/v) to the culture medium. However, at 5% (w/v) yeast extract concentration, proteolytic activity is not detected and consequently, the activity of free lipase may easily be measured. Free lipase activity can easily be detected when 0·001 mol dm−3 EDTA is added to the culture medium. The presence of the chelating agent enhances the production and maintains the stability of the extracted mycelium-bound lipase. |
| first_indexed | 2025-11-15T09:58:55Z |
| format | Article |
| id | upm-42480 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:58:55Z |
| publishDate | 1996 |
| publisher | Society of Chemical Industry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-424802024-08-05T03:07:21Z http://psasir.upm.edu.my/id/eprint/42480/ Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Ampon, Kamaruzaman Bucke, Christopher Aspergillus flavus produces a lipase (EC 3.1.1.3) which is partly bound to the mycelium during growth. The production of the mycelium-bound lipase is concomitant with growth, and declines when growth ceases. Maximum productivity of the enzyme is obtained when the culture is incubated at 30°C, an initial culture pH of 6·5 and with 2% (w/v) each of corn oil and yeast extract as carbon and organic nitrogen source. Yeast extract affects not only the production of lipase but also the secretion of proteases into the culture medium. Production of the latter enzymes, which inactivate the free lipase, is enhanced by adding yeast extract (1–2%, w/v) to the culture medium. However, at 5% (w/v) yeast extract concentration, proteolytic activity is not detected and consequently, the activity of free lipase may easily be measured. Free lipase activity can easily be detected when 0·001 mol dm−3 EDTA is added to the culture medium. The presence of the chelating agent enhances the production and maintains the stability of the extracted mycelium-bound lipase. Society of Chemical Industry 1996-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/42480/1/Mycelium-bound%20lipase%20from%20a%20locally%20isolated%20strain%20of%20Aspergillus%20flavus%20link%20pattern%20and%20factors%20involved%20in%20its%20production.pdf text en http://psasir.upm.edu.my/id/eprint/42480/7/J%20of%20Chemical%20Tech%20%20%20Biotech%20-%20October%201996%20-%20Long%20-%20Mycelium%E2%80%90bound%20lipase%20from%20a%20locally%20isolated%20strain%20of%20Aspergillus.pdf Long, Kamariah and Mohd Ghazali, Hasanah and Ariff, Arbakariya and Ampon, Kamaruzaman and Bucke, Christopher (1996) Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production. Journal of Chemical Technology and Biotechnology, 67 (2). pp. 157-163. ISSN 0268-2575; ESSN: 1097-4660 http://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291097-4660%28199610%2967:2%3C157::AID-JCTB533%3E3.0.CO;2-I/abstract 10.1002/(SICI)1097-4660(199610)67:2<157::AID-JCTB533>3.0.CO;2-I |
| spellingShingle | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Ampon, Kamaruzaman Bucke, Christopher Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production |
| title | Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production |
| title_full | Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production |
| title_fullStr | Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production |
| title_full_unstemmed | Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production |
| title_short | Mycelium-bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production |
| title_sort | mycelium-bound lipase from a locally isolated strain of aspergillus flavus link: pattern and factors involved in its production |
| url | http://psasir.upm.edu.my/id/eprint/42480/ http://psasir.upm.edu.my/id/eprint/42480/ http://psasir.upm.edu.my/id/eprint/42480/ http://psasir.upm.edu.my/id/eprint/42480/1/Mycelium-bound%20lipase%20from%20a%20locally%20isolated%20strain%20of%20Aspergillus%20flavus%20link%20pattern%20and%20factors%20involved%20in%20its%20production.pdf http://psasir.upm.edu.my/id/eprint/42480/7/J%20of%20Chemical%20Tech%20%20%20Biotech%20-%20October%201996%20-%20Long%20-%20Mycelium%E2%80%90bound%20lipase%20from%20a%20locally%20isolated%20strain%20of%20Aspergillus.pdf |