Aquilaria malaccensis polyploids as improved planting materials
Aquilaria malaccensis is an agarwood-producing timber species used in many traditional remedies and modern therapeutic treatments and perfume industries. In this study, we aimed to enhance A. malaccensis phytochemical content through in-vitro polyploidisation. Shoot tip and nodal segment from 8-week...
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| Format: | Article |
| Language: | English |
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Forest Research Institute Malaysia
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/42290/ http://psasir.upm.edu.my/id/eprint/42290/1/Aquilaria%20malaccensis%20polyploids%20as%20improved%20planting%20materials.pdf |
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| author | A. Rahman, Siti Suhaila Mohd Saleh, Norihan Muhammad, Norwati Mohamad Ali, Nor Azah Mansor Clyde, Mahani Namasivayam, Parameswari Kodi, I. K. Jamil, Mailina Azlan, Azrina Hassan, Nor Hasnida Ismail, Haliza Abdullah, Nazirah Yahya, Muhammad Fuad |
| author_facet | A. Rahman, Siti Suhaila Mohd Saleh, Norihan Muhammad, Norwati Mohamad Ali, Nor Azah Mansor Clyde, Mahani Namasivayam, Parameswari Kodi, I. K. Jamil, Mailina Azlan, Azrina Hassan, Nor Hasnida Ismail, Haliza Abdullah, Nazirah Yahya, Muhammad Fuad |
| author_sort | A. Rahman, Siti Suhaila |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aquilaria malaccensis is an agarwood-producing timber species used in many traditional remedies and modern therapeutic treatments and perfume industries. In this study, we aimed to enhance A. malaccensis phytochemical content through in-vitro polyploidisation. Shoot tip and nodal segment from 8-week-old in-vitro A. malaccensis plantlets were treated with different concentrations of colchicine and trifluralin at various exposure times to obtain polyploids. Tetraploid plantlets (10%) was obtained using nodal segment explants treated with 0.1 mM trifluralin at 120 hours. Chemical profiling of diploid and tetraploid samples (leaf, stem and root) was evaluated separately using headspace-solid phase microextraction (HS-SPME) combined with gas chromatograph mass spectrometry (GCMS). Phytochemical content increased in tetraploid, particularly in stem whereby the total phytochemical contents were 43.19% in tetraploid compared with 5.87% in diploid. The HS-SPME-GCMS analyses showed that tetraploid stem contained high levels of sesquiterpenoids found in agarwood oil such as α-eudesmol (18.3%), α-gurjunene (8.61%) and γ-gurjunene (6.22%). On the other hand, aromadendrene (2.49%) and α-humulene (3.38%) were detected in diploid samples. Tetraploid leaf samples were observed to contain α-humulene (3.79%) while diploid only contained (2E) tridecenol (19%). There were no significant differences between diploid and tetraploid in terms of total phytochemical content in root samples. Nevertheless, high sesquiterpenoid content, γ-gurjunene (14.0%), was detected in tetraploid sample while γ-muurolene (2.96%), in diploid. α-Guaiene content was higher in root samples of diploid (6.49%) than tetraploid (1.09%). These results demonstrated that tetraploid plantlets led to higher yield of total phytochemical content and might facilitate production of high quality A. malaccensis clones. |
| first_indexed | 2025-11-15T09:58:06Z |
| format | Article |
| id | upm-42290 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:58:06Z |
| publishDate | 2015 |
| publisher | Forest Research Institute Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-422902016-04-12T08:09:05Z http://psasir.upm.edu.my/id/eprint/42290/ Aquilaria malaccensis polyploids as improved planting materials A. Rahman, Siti Suhaila Mohd Saleh, Norihan Muhammad, Norwati Mohamad Ali, Nor Azah Mansor Clyde, Mahani Namasivayam, Parameswari Kodi, I. K. Jamil, Mailina Azlan, Azrina Hassan, Nor Hasnida Ismail, Haliza Abdullah, Nazirah Yahya, Muhammad Fuad Aquilaria malaccensis is an agarwood-producing timber species used in many traditional remedies and modern therapeutic treatments and perfume industries. In this study, we aimed to enhance A. malaccensis phytochemical content through in-vitro polyploidisation. Shoot tip and nodal segment from 8-week-old in-vitro A. malaccensis plantlets were treated with different concentrations of colchicine and trifluralin at various exposure times to obtain polyploids. Tetraploid plantlets (10%) was obtained using nodal segment explants treated with 0.1 mM trifluralin at 120 hours. Chemical profiling of diploid and tetraploid samples (leaf, stem and root) was evaluated separately using headspace-solid phase microextraction (HS-SPME) combined with gas chromatograph mass spectrometry (GCMS). Phytochemical content increased in tetraploid, particularly in stem whereby the total phytochemical contents were 43.19% in tetraploid compared with 5.87% in diploid. The HS-SPME-GCMS analyses showed that tetraploid stem contained high levels of sesquiterpenoids found in agarwood oil such as α-eudesmol (18.3%), α-gurjunene (8.61%) and γ-gurjunene (6.22%). On the other hand, aromadendrene (2.49%) and α-humulene (3.38%) were detected in diploid samples. Tetraploid leaf samples were observed to contain α-humulene (3.79%) while diploid only contained (2E) tridecenol (19%). There were no significant differences between diploid and tetraploid in terms of total phytochemical content in root samples. Nevertheless, high sesquiterpenoid content, γ-gurjunene (14.0%), was detected in tetraploid sample while γ-muurolene (2.96%), in diploid. α-Guaiene content was higher in root samples of diploid (6.49%) than tetraploid (1.09%). These results demonstrated that tetraploid plantlets led to higher yield of total phytochemical content and might facilitate production of high quality A. malaccensis clones. Forest Research Institute Malaysia 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/42290/1/Aquilaria%20malaccensis%20polyploids%20as%20improved%20planting%20materials.pdf A. Rahman, Siti Suhaila and Mohd Saleh, Norihan and Muhammad, Norwati and Mohamad Ali, Nor Azah and Mansor Clyde, Mahani and Namasivayam, Parameswari and Kodi, I. K. and Jamil, Mailina and Azlan, Azrina and Hassan, Nor Hasnida and Ismail, Haliza and Abdullah, Nazirah and Yahya, Muhammad Fuad (2015) Aquilaria malaccensis polyploids as improved planting materials. Journal of Tropical Forest Science, 27 (3). pp. 376-387. ISSN 0128-1283 http://www.frim.gov.my/publication/journal-of-tropical-forest-science-jtfs/ |
| spellingShingle | A. Rahman, Siti Suhaila Mohd Saleh, Norihan Muhammad, Norwati Mohamad Ali, Nor Azah Mansor Clyde, Mahani Namasivayam, Parameswari Kodi, I. K. Jamil, Mailina Azlan, Azrina Hassan, Nor Hasnida Ismail, Haliza Abdullah, Nazirah Yahya, Muhammad Fuad Aquilaria malaccensis polyploids as improved planting materials |
| title | Aquilaria malaccensis polyploids as improved planting materials |
| title_full | Aquilaria malaccensis polyploids as improved planting materials |
| title_fullStr | Aquilaria malaccensis polyploids as improved planting materials |
| title_full_unstemmed | Aquilaria malaccensis polyploids as improved planting materials |
| title_short | Aquilaria malaccensis polyploids as improved planting materials |
| title_sort | aquilaria malaccensis polyploids as improved planting materials |
| url | http://psasir.upm.edu.my/id/eprint/42290/ http://psasir.upm.edu.my/id/eprint/42290/ http://psasir.upm.edu.my/id/eprint/42290/1/Aquilaria%20malaccensis%20polyploids%20as%20improved%20planting%20materials.pdf |