Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells
Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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PeerJ
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/42279/ http://psasir.upm.edu.my/id/eprint/42279/1/42279.pdf |
| _version_ | 1848849920676593664 |
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| author | Kong, Kin Weng Mat Junit, Sarni Aminudin, Norhaniza Hassan, Fouad Abdulrahman Ismail, Amin Abdul Aziz, Azlina |
| author_facet | Kong, Kin Weng Mat Junit, Sarni Aminudin, Norhaniza Hassan, Fouad Abdulrahman Ismail, Amin Abdul Aziz, Azlina |
| author_sort | Kong, Kin Weng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage. |
| first_indexed | 2025-11-15T09:58:04Z |
| format | Article |
| id | upm-42279 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:58:04Z |
| publishDate | 2016 |
| publisher | PeerJ |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-422792016-04-12T07:55:45Z http://psasir.upm.edu.my/id/eprint/42279/ Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells Kong, Kin Weng Mat Junit, Sarni Aminudin, Norhaniza Hassan, Fouad Abdulrahman Ismail, Amin Abdul Aziz, Azlina Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage. PeerJ 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/42279/1/42279.pdf Kong, Kin Weng and Mat Junit, Sarni and Aminudin, Norhaniza and Hassan, Fouad Abdulrahman and Ismail, Amin and Abdul Aziz, Azlina (2016) Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells. PeerJ, 4. art. no. 1628. pp. 1-20. ISSN 2167-8359 https://peerj.com/ 10.7717/peerj.1628 |
| spellingShingle | Kong, Kin Weng Mat Junit, Sarni Aminudin, Norhaniza Hassan, Fouad Abdulrahman Ismail, Amin Abdul Aziz, Azlina Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells |
| title | Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells |
| title_full | Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells |
| title_fullStr | Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells |
| title_full_unstemmed | Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells |
| title_short | Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells |
| title_sort | protective effects of the extracts of barringtonia racemosa shoots against oxidative damage in hepg2 cells |
| url | http://psasir.upm.edu.my/id/eprint/42279/ http://psasir.upm.edu.my/id/eprint/42279/ http://psasir.upm.edu.my/id/eprint/42279/ http://psasir.upm.edu.my/id/eprint/42279/1/42279.pdf |