Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis
The present research was designed to evaluate the anticancer properties of Dillenia suffruticosa extract. Our focus was on the mode of cell death and cell cycle arrest induced in breast cancer cells by the active fractions (designated as D/F4, D/F5 and EA/P2) derived from chromatographic fractionati...
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| Format: | Article |
| Language: | English |
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MDPI
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41931/ http://psasir.upm.edu.my/id/eprint/41931/1/41931.pdf |
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| author | Armania, Nurdin Saiful Yazan, Latifah Ismail, Intan Safinar Foo, Jhi Biau Tor, Yim Sim Ishak, Nurshafini Ismail, Norsharina Ismail, Maznah |
| author_facet | Armania, Nurdin Saiful Yazan, Latifah Ismail, Intan Safinar Foo, Jhi Biau Tor, Yim Sim Ishak, Nurshafini Ismail, Norsharina Ismail, Maznah |
| author_sort | Armania, Nurdin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present research was designed to evaluate the anticancer properties of Dillenia suffruticosa extract. Our focus was on the mode of cell death and cell cycle arrest induced in breast cancer cells by the active fractions (designated as D/F4, D/F5 and EA/P2) derived from chromatographic fractionation of D. suffruticosa extracts. The results showed that the active fractions are more cytotoxic towards MCF-7 (estrogen positive breast cancer cells) and MDA-MB-231 (estrogen negative breast cancer cells) as compared to other selected cancer cell lines that included HeLa, A459 and CaOV3. The induction of cell death through apoptosis by the active fractions on the breast cancer cells was confirmed by Annexin V-FITC and PI staining. Cell cycle analysis revealed that D/F4 and EA/P2 induced G2/M phase cell cycle arrest in MCF-7 cells. On the other hand, MDA-MB-231 cells treated with D/F4 and D/F5 accumulated in the sub-G1 phase without cell cycle arrest, suggesting the induction of cell death through apoptosis. The data suggest that the active fractions of D. suffruticosa extract eliminated breast cancer cells through induction of apoptosis and cell cycle arrest. The reason why MCF-7 was more sensitive towards the treatment than MDA-MB-231 remains unclear. This warrants further work, especially on the role of hormones in response towards cytotoxic agents. In addition, more studies on the mechanisms underlying the induction of apoptosis and cell cycle arrest by the plant extract also need to be carried out. |
| first_indexed | 2025-11-15T09:56:37Z |
| format | Article |
| id | upm-41931 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:56:37Z |
| publishDate | 2013 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-419312016-02-10T03:57:03Z http://psasir.upm.edu.my/id/eprint/41931/ Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis Armania, Nurdin Saiful Yazan, Latifah Ismail, Intan Safinar Foo, Jhi Biau Tor, Yim Sim Ishak, Nurshafini Ismail, Norsharina Ismail, Maznah The present research was designed to evaluate the anticancer properties of Dillenia suffruticosa extract. Our focus was on the mode of cell death and cell cycle arrest induced in breast cancer cells by the active fractions (designated as D/F4, D/F5 and EA/P2) derived from chromatographic fractionation of D. suffruticosa extracts. The results showed that the active fractions are more cytotoxic towards MCF-7 (estrogen positive breast cancer cells) and MDA-MB-231 (estrogen negative breast cancer cells) as compared to other selected cancer cell lines that included HeLa, A459 and CaOV3. The induction of cell death through apoptosis by the active fractions on the breast cancer cells was confirmed by Annexin V-FITC and PI staining. Cell cycle analysis revealed that D/F4 and EA/P2 induced G2/M phase cell cycle arrest in MCF-7 cells. On the other hand, MDA-MB-231 cells treated with D/F4 and D/F5 accumulated in the sub-G1 phase without cell cycle arrest, suggesting the induction of cell death through apoptosis. The data suggest that the active fractions of D. suffruticosa extract eliminated breast cancer cells through induction of apoptosis and cell cycle arrest. The reason why MCF-7 was more sensitive towards the treatment than MDA-MB-231 remains unclear. This warrants further work, especially on the role of hormones in response towards cytotoxic agents. In addition, more studies on the mechanisms underlying the induction of apoptosis and cell cycle arrest by the plant extract also need to be carried out. MDPI 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41931/1/41931.pdf Armania, Nurdin and Saiful Yazan, Latifah and Ismail, Intan Safinar and Foo, Jhi Biau and Tor, Yim Sim and Ishak, Nurshafini and Ismail, Norsharina and Ismail, Maznah (2013) Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis. Molecules, 18 (11). pp. 13320-13339. ISSN 1420-3049 http://www.mdpi.com/1420-3049/18/11/13320 10.3390/molecules181113320 |
| spellingShingle | Armania, Nurdin Saiful Yazan, Latifah Ismail, Intan Safinar Foo, Jhi Biau Tor, Yim Sim Ishak, Nurshafini Ismail, Norsharina Ismail, Maznah Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis |
| title | Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis |
| title_full | Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis |
| title_fullStr | Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis |
| title_full_unstemmed | Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis |
| title_short | Dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (MCF-7 and MDA-MB-231) via induction of G2/M arrest and apoptosis |
| title_sort | dillenia suffruticosa extract inhibits proliferation of human breast cancer cell lines (mcf-7 and mda-mb-231) via induction of g2/m arrest and apoptosis |
| url | http://psasir.upm.edu.my/id/eprint/41931/ http://psasir.upm.edu.my/id/eprint/41931/ http://psasir.upm.edu.my/id/eprint/41931/ http://psasir.upm.edu.my/id/eprint/41931/1/41931.pdf |