Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin
In this study, tumorspheres were generated from TW06 nasopharyngeal carcinoma cell line and examined their expression of putative cancer stem-like cell surface markers and drug sensitivity. The rate of tumorsphere expansion from dissociated late passage TW06 tumorspheres (≥ passage 15) was higher th...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41695/ http://psasir.upm.edu.my/id/eprint/41695/1/Characterization%20of%20tumorspheres%20generated%20from%20nasopharyngeal%20carcinoma.pdf |
| _version_ | 1848849764935794688 |
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| author | Goh, Iris Wen Li Yip, Wai Kien Seow, Heng Fong |
| author_facet | Goh, Iris Wen Li Yip, Wai Kien Seow, Heng Fong |
| author_sort | Goh, Iris Wen Li |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, tumorspheres were generated from TW06 nasopharyngeal carcinoma cell line and examined their expression of putative cancer stem-like cell surface markers and drug sensitivity. The rate of tumorsphere expansion from dissociated late passage TW06 tumorspheres (≥ passage 15) was higher than that from parental cells and dissociated 10-day-old (passage 0) tumorspheres. The expression of CD24 surface marker was lost in the generation of tumorspheres and the loss was reversible after differentiating the tumorspheres in monolayer culture conditions. Drug sensitivity assay showed that late passage tumorspheres were resistant to docetaxel and oxaliplatin treatment. Our data suggest that serially passaged tumorspheres possess the characteristics of CSCs that render them a suitable preclinical in vitro model for evaluating anticancer drug efficacy and elucidating the underlying mechanisms of drug resistance. |
| first_indexed | 2025-11-15T09:55:35Z |
| format | Article |
| id | upm-41695 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:55:35Z |
| publishDate | 2015 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-416952016-02-01T06:33:46Z http://psasir.upm.edu.my/id/eprint/41695/ Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin Goh, Iris Wen Li Yip, Wai Kien Seow, Heng Fong In this study, tumorspheres were generated from TW06 nasopharyngeal carcinoma cell line and examined their expression of putative cancer stem-like cell surface markers and drug sensitivity. The rate of tumorsphere expansion from dissociated late passage TW06 tumorspheres (≥ passage 15) was higher than that from parental cells and dissociated 10-day-old (passage 0) tumorspheres. The expression of CD24 surface marker was lost in the generation of tumorspheres and the loss was reversible after differentiating the tumorspheres in monolayer culture conditions. Drug sensitivity assay showed that late passage tumorspheres were resistant to docetaxel and oxaliplatin treatment. Our data suggest that serially passaged tumorspheres possess the characteristics of CSCs that render them a suitable preclinical in vitro model for evaluating anticancer drug efficacy and elucidating the underlying mechanisms of drug resistance. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2015-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41695/1/Characterization%20of%20tumorspheres%20generated%20from%20nasopharyngeal%20carcinoma.pdf Goh, Iris Wen Li and Yip, Wai Kien and Seow, Heng Fong (2015) Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin. Malaysian Journal of Medicine and Health Sciences, 11 (2). pp. 9-20. ISSN 1675-8544 http://www.medic.upm.edu.my/dokumen/FKUSK1_Final_Article_2.pdf |
| spellingShingle | Goh, Iris Wen Li Yip, Wai Kien Seow, Heng Fong Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin |
| title | Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin |
| title_full | Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin |
| title_fullStr | Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin |
| title_full_unstemmed | Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin |
| title_short | Characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, TW06 and chemoresistance to docetaxel and oxaliplatin |
| title_sort | characterization of tumorspheres generated from nasopharyngeal carcinoma cell line, tw06 and chemoresistance to docetaxel and oxaliplatin |
| url | http://psasir.upm.edu.my/id/eprint/41695/ http://psasir.upm.edu.my/id/eprint/41695/ http://psasir.upm.edu.my/id/eprint/41695/1/Characterization%20of%20tumorspheres%20generated%20from%20nasopharyngeal%20carcinoma.pdf |