Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates
The amino acid sequence of the fusion (F) protein of Newcastle disease virus (NDV) is of particular interest as its virulence is highly dependent on susceptibility to proteolytic cleavage. The F protein precursor, F0' can beproteolytically cleaved to form disulfide-linked F, and F2 chains. The...
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| Format: | Article |
| Language: | English |
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Veterinary Association Malaysia
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41473/ http://psasir.upm.edu.my/id/eprint/41473/1/0001.pdf |
| _version_ | 1848849705478389760 |
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| author | Ramanujam, Priadarishni Tan, Wen Siang Nathan, Sheila Yusoff, Khatijah |
| author_facet | Ramanujam, Priadarishni Tan, Wen Siang Nathan, Sheila Yusoff, Khatijah |
| author_sort | Ramanujam, Priadarishni |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The amino acid sequence of the fusion (F) protein of Newcastle disease virus (NDV) is of particular interest as its virulence is highly dependent on susceptibility to proteolytic cleavage. The F protein precursor, F0' can beproteolytically cleaved to form disulfide-linked F, and F2 chains. The Fa cleavage is a prerequisite for producing infectious particles
whereby the nature of the cleavage site correlates with virulence of the virus. Inthis study we determined the F protein gene cleavage site nucleotide sequence ofNDV local isolates 2641191 P2, 5953/89 P3, 1266/89 P3 and 8820/92 P3. Their sequences were also compared with other NDV strains and isolates, such as AF2240, OOIIKS, 011TM, 01lC, 3410/92 P2, 6385/90 P3, V4 Que and F that had previously been determined. The deduced amino acid sequence of this site revealed the pathogenecity of these strains whereby 2641191 P2 and 5953/89 P3 were classified as virulent isolates whilst 1266/89 P3 and 8820/92 P3 were avirulent isolates.
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| first_indexed | 2025-11-15T09:54:38Z |
| format | Article |
| id | upm-41473 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:54:38Z |
| publishDate | 2004 |
| publisher | Veterinary Association Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-414732015-12-21T07:52:25Z http://psasir.upm.edu.my/id/eprint/41473/ Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates Ramanujam, Priadarishni Tan, Wen Siang Nathan, Sheila Yusoff, Khatijah The amino acid sequence of the fusion (F) protein of Newcastle disease virus (NDV) is of particular interest as its virulence is highly dependent on susceptibility to proteolytic cleavage. The F protein precursor, F0' can beproteolytically cleaved to form disulfide-linked F, and F2 chains. The Fa cleavage is a prerequisite for producing infectious particles whereby the nature of the cleavage site correlates with virulence of the virus. Inthis study we determined the F protein gene cleavage site nucleotide sequence ofNDV local isolates 2641191 P2, 5953/89 P3, 1266/89 P3 and 8820/92 P3. Their sequences were also compared with other NDV strains and isolates, such as AF2240, OOIIKS, 011TM, 01lC, 3410/92 P2, 6385/90 P3, V4 Que and F that had previously been determined. The deduced amino acid sequence of this site revealed the pathogenecity of these strains whereby 2641191 P2 and 5953/89 P3 were classified as virulent isolates whilst 1266/89 P3 and 8820/92 P3 were avirulent isolates. Veterinary Association Malaysia 2004 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41473/1/0001.pdf Ramanujam, Priadarishni and Tan, Wen Siang and Nathan, Sheila and Yusoff, Khatijah (2004) Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates. Jurnal Veterinar Malaysia, 16 (1&2). pp. 9-13. ISSN 9128-2506 |
| spellingShingle | Ramanujam, Priadarishni Tan, Wen Siang Nathan, Sheila Yusoff, Khatijah Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates |
| title | Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates |
| title_full | Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates |
| title_fullStr | Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates |
| title_full_unstemmed | Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates |
| title_short | Sequence analysis of the fusion (f) protein cleavage site of four Newcastle disease virus isolates |
| title_sort | sequence analysis of the fusion (f) protein cleavage site of four newcastle disease virus isolates |
| url | http://psasir.upm.edu.my/id/eprint/41473/ http://psasir.upm.edu.my/id/eprint/41473/1/0001.pdf |