Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection
Previous studies have identified differential expression of immune-mediated genes related to inflammatory response in chickens with different genetic susceptibility to IBDV infections. However, the mechanisms of genetic resistance against IBD are not known. RNA sequencing through NGS technologies pr...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41414/ http://psasir.upm.edu.my/id/eprint/41414/1/41414.pdf |
| _version_ | 1848849688883625984 |
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| author | Mohd Isa, Nor Farah Bejo, Mohd Hair Mat Isa, Nurulfiza Ideris, Aini Nair, Venugopal Omar, Abdul Rahman |
| author_facet | Mohd Isa, Nor Farah Bejo, Mohd Hair Mat Isa, Nurulfiza Ideris, Aini Nair, Venugopal Omar, Abdul Rahman |
| author_sort | Mohd Isa, Nor Farah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Previous studies have identified differential expression of immune-mediated genes related to inflammatory response in chickens with different genetic susceptibility to IBDV infections. However, the mechanisms of genetic resistance against IBD are not known. RNA sequencing through NGS technologies provide an excellent platform to study differentially expressed genes of known or unknown functions to better define mechanisms of host resistance. Therefore, this study aimed at investigating susceptibility of different inbred chicken lines toward very virulent IBDV through transcriptomic analysis. This analysis allows for quantification and comparison of gene expression. Bioinformatics analysis of this data will allow function annotation of differentially expressed genes, indicating possible roles in the response to infection. Gene of interest, virus load detection, indel, SNP and CNV between different lines and breeds will be validated using qPCR. This study is expected to provide information that able to decipher the genetic resistance of chickens towards IBDV infection. |
| first_indexed | 2025-11-15T09:54:23Z |
| format | Conference or Workshop Item |
| id | upm-41414 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:54:23Z |
| publishDate | 2013 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-414142016-01-28T02:04:41Z http://psasir.upm.edu.my/id/eprint/41414/ Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection Mohd Isa, Nor Farah Bejo, Mohd Hair Mat Isa, Nurulfiza Ideris, Aini Nair, Venugopal Omar, Abdul Rahman Previous studies have identified differential expression of immune-mediated genes related to inflammatory response in chickens with different genetic susceptibility to IBDV infections. However, the mechanisms of genetic resistance against IBD are not known. RNA sequencing through NGS technologies provide an excellent platform to study differentially expressed genes of known or unknown functions to better define mechanisms of host resistance. Therefore, this study aimed at investigating susceptibility of different inbred chicken lines toward very virulent IBDV through transcriptomic analysis. This analysis allows for quantification and comparison of gene expression. Bioinformatics analysis of this data will allow function annotation of differentially expressed genes, indicating possible roles in the response to infection. Gene of interest, virus load detection, indel, SNP and CNV between different lines and breeds will be validated using qPCR. This study is expected to provide information that able to decipher the genetic resistance of chickens towards IBDV infection. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41414/1/41414.pdf Mohd Isa, Nor Farah and Bejo, Mohd Hair and Mat Isa, Nurulfiza and Ideris, Aini and Nair, Venugopal and Omar, Abdul Rahman (2013) Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 126-127). http://www.vet.upm.edu.my/dokumen/90301_proceeding_WPSA_V2_first_second_XX_new_20121013_%281%29.pdf |
| spellingShingle | Mohd Isa, Nor Farah Bejo, Mohd Hair Mat Isa, Nurulfiza Ideris, Aini Nair, Venugopal Omar, Abdul Rahman Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection |
| title | Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection |
| title_full | Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection |
| title_fullStr | Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection |
| title_full_unstemmed | Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection |
| title_short | Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection |
| title_sort | transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection |
| url | http://psasir.upm.edu.my/id/eprint/41414/ http://psasir.upm.edu.my/id/eprint/41414/ http://psasir.upm.edu.my/id/eprint/41414/1/41414.pdf |