Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site

Intensive vaccine programs have been implemented in many countries including Malaysia, but Newcastle disease virus (NDV) outbreaks have occurred, even in well-vaccinated farms. Hence, the present study was aimed to characterize five NDV isolates obtained from NDV vaccinated broiler farms in 2011 bas...

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Main Authors: Shahrestani, Kiarash Roohani, Ideris, Aini, Bejo, Mohd Hair, Tan, Sheau Wei, Yeap, Swee Keong, Rasoli, Mehdi, Jazayeri, Seyed Davoud, Goh, Su Hua, Omar, Abdul Rahman
Format: Conference or Workshop Item
Language:English
Published: Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013
Online Access:http://psasir.upm.edu.my/id/eprint/41411/
http://psasir.upm.edu.my/id/eprint/41411/1/41411.pdf
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author Shahrestani, Kiarash Roohani
Ideris, Aini
Bejo, Mohd Hair
Tan, Sheau Wei
Yeap, Swee Keong
Rasoli, Mehdi
Jazayeri, Seyed Davoud
Goh, Su Hua
Omar, Abdul Rahman
author_facet Shahrestani, Kiarash Roohani
Ideris, Aini
Bejo, Mohd Hair
Tan, Sheau Wei
Yeap, Swee Keong
Rasoli, Mehdi
Jazayeri, Seyed Davoud
Goh, Su Hua
Omar, Abdul Rahman
author_sort Shahrestani, Kiarash Roohani
building UPM Institutional Repository
collection Online Access
description Intensive vaccine programs have been implemented in many countries including Malaysia, but Newcastle disease virus (NDV) outbreaks have occurred, even in well-vaccinated farms. Hence, the present study was aimed to characterize five NDV isolates obtained from NDV vaccinated broiler farms in 2011 based on sequence and phylogenetic analysis of partial fusion gene. All the isolated NDV strains showed that they are categorized as velogenic NDV based on the presence of multi-basic protease cleavage sites, 112RRRKRF117. In addition, phylogenetic analysis showed that the isolates can classified under the genotype VII, subgenotype VIId.
first_indexed 2025-11-15T09:54:22Z
format Conference or Workshop Item
id upm-41411
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T09:54:22Z
publishDate 2013
publisher Faculty of Veterinary Medicine, Universiti Putra Malaysia
recordtype eprints
repository_type Digital Repository
spelling upm-414112016-01-28T01:54:50Z http://psasir.upm.edu.my/id/eprint/41411/ Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site Shahrestani, Kiarash Roohani Ideris, Aini Bejo, Mohd Hair Tan, Sheau Wei Yeap, Swee Keong Rasoli, Mehdi Jazayeri, Seyed Davoud Goh, Su Hua Omar, Abdul Rahman Intensive vaccine programs have been implemented in many countries including Malaysia, but Newcastle disease virus (NDV) outbreaks have occurred, even in well-vaccinated farms. Hence, the present study was aimed to characterize five NDV isolates obtained from NDV vaccinated broiler farms in 2011 based on sequence and phylogenetic analysis of partial fusion gene. All the isolated NDV strains showed that they are categorized as velogenic NDV based on the presence of multi-basic protease cleavage sites, 112RRRKRF117. In addition, phylogenetic analysis showed that the isolates can classified under the genotype VII, subgenotype VIId. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41411/1/41411.pdf Shahrestani, Kiarash Roohani and Ideris, Aini and Bejo, Mohd Hair and Tan, Sheau Wei and Yeap, Swee Keong and Rasoli, Mehdi and Jazayeri, Seyed Davoud and Goh, Su Hua and Omar, Abdul Rahman (2013) Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 117-119). http://www.vet.upm.edu.my/dokumen/90301_proceeding_WPSA_V2_first_second_XX_new_20121013_%281%29.pdf
spellingShingle Shahrestani, Kiarash Roohani
Ideris, Aini
Bejo, Mohd Hair
Tan, Sheau Wei
Yeap, Swee Keong
Rasoli, Mehdi
Jazayeri, Seyed Davoud
Goh, Su Hua
Omar, Abdul Rahman
Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site
title Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site
title_full Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site
title_fullStr Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site
title_full_unstemmed Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site
title_short Molecular characterisation and pathotyping of recently isolated Newcastle disease virus isolates based on F protein’s cleavage site
title_sort molecular characterisation and pathotyping of recently isolated newcastle disease virus isolates based on f protein’s cleavage site
url http://psasir.upm.edu.my/id/eprint/41411/
http://psasir.upm.edu.my/id/eprint/41411/
http://psasir.upm.edu.my/id/eprint/41411/1/41411.pdf