Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene
The significance of the role of mitochondria incontending viral infection is gaining worldwide interest. Its ability to elicit molecular signals to other immune components enables new perspective in the development of novel antiviral agents. This study explored the possibility of isolating the mitoc...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41408/ http://psasir.upm.edu.my/id/eprint/41408/2/41408.pdf |
| _version_ | 1848849687236313088 |
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| author | Mohamad Wali, Haryati Shila Ideris, Aini Omar, Abdul Rahman Bejo, Mohd Hair |
| author_facet | Mohamad Wali, Haryati Shila Ideris, Aini Omar, Abdul Rahman Bejo, Mohd Hair |
| author_sort | Mohamad Wali, Haryati Shila |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The significance of the role of mitochondria incontending viral infection is gaining worldwide interest. Its ability to elicit molecular signals to other immune components enables new perspective in the development of novel antiviral agents. This study explored the possibility of isolating the mitochondrial antiviral signaling (MAVS) gene from chicken tissue and cloning it into cloning vector. Prior to cloning, RNA extraction was carried out from chicken samples and converted to cDNA. Polymerase chain reaction (PCR) amplified product was ligated to the vector pJET1.2. The resulting plasmid construct pJET1.2/CARDIF was then transformed into competent cells and later sequenced and analysed by restriction enzyme analysis. BLAST analysis of the construct yields 99% similarity with Gallus gallus CARDIF mRNA gene. |
| first_indexed | 2025-11-15T09:54:21Z |
| format | Conference or Workshop Item |
| id | upm-41408 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:54:21Z |
| publishDate | 2013 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-414082016-01-28T01:39:39Z http://psasir.upm.edu.my/id/eprint/41408/ Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene Mohamad Wali, Haryati Shila Ideris, Aini Omar, Abdul Rahman Bejo, Mohd Hair The significance of the role of mitochondria incontending viral infection is gaining worldwide interest. Its ability to elicit molecular signals to other immune components enables new perspective in the development of novel antiviral agents. This study explored the possibility of isolating the mitochondrial antiviral signaling (MAVS) gene from chicken tissue and cloning it into cloning vector. Prior to cloning, RNA extraction was carried out from chicken samples and converted to cDNA. Polymerase chain reaction (PCR) amplified product was ligated to the vector pJET1.2. The resulting plasmid construct pJET1.2/CARDIF was then transformed into competent cells and later sequenced and analysed by restriction enzyme analysis. BLAST analysis of the construct yields 99% similarity with Gallus gallus CARDIF mRNA gene. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41408/2/41408.pdf Mohamad Wali, Haryati Shila and Ideris, Aini and Omar, Abdul Rahman and Bejo, Mohd Hair (2013) Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 111-112). http://www.vet.upm.edu.my/dokumen/90301_proceeding_WPSA_V2_first_second_XX_new_20121013_%281%29.pdf |
| spellingShingle | Mohamad Wali, Haryati Shila Ideris, Aini Omar, Abdul Rahman Bejo, Mohd Hair Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene |
| title | Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene |
| title_full | Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene |
| title_fullStr | Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene |
| title_full_unstemmed | Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene |
| title_short | Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene |
| title_sort | construction of a plasmid encoding chicken mitochondrial antiviral signaling (mavs) gene |
| url | http://psasir.upm.edu.my/id/eprint/41408/ http://psasir.upm.edu.my/id/eprint/41408/ http://psasir.upm.edu.my/id/eprint/41408/2/41408.pdf |