Development of in situ PCR technique for detection of very virulent infectious bursal disease virus strain

QR Code

Development of in situ PCR technique for detection of very virulent infectious bursal disease virus strain

A very virulent infectious bursal disease virus (vvIBDV) strain (UPM0081) was inoculated orally in 4-week-old specific pathogen free (SPF) chickens. Control was included and these chickens were not inoculated with the virus. The chickens in both groups were sacrificed at various intervals and sample...

Full description

Bibliographic Details
Main Authors:	Elawad, H. A., Bejo, Mohd Hair, Omar, Abdul Rahman, Arshad, Siti Suri, Ideris, Aini
Format:	Conference or Workshop Item
Language:	English
Published:	Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/41395/ http://psasir.upm.edu.my/id/eprint/41395/1/41395.pdf

Similar Items

In situ PCR for detection and differentiation of infectious bursal disease virus strains in chickens
by: Hussein, Elawad A., et al.
Published: (2017)

Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
by: Mohd Isa, Noor Farhanah, et al.
Published: (2015)

Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia
by: Liew, Pit Sze, et al.
Published: (2013)

Development of in situ PCR for virus detection, strain differentiation and pathogenesis of newcastle disease and infectious bursal disease in chickens
by: Mahmoud, Hussein Abdallah Elawad
Published: (2017)

Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus
by: Kong, Lih Ling, et al.
Published: (2004)

Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining
by: Elawad, Hussein Abdallah, et al.
Published: (2019)

Adaptation and molecular characterization of two Malaysian very virulent infectious bursal disease virus isolates adapted in BGM-70 cell line
by: Lawal, Nafi’u, et al.
Published: (2017)

Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus
by: Lih, Ling Kong, et al.
Published: (2009)

Replication of very virulent infectious bursal disease virus in primary cells of the chick embryo chorioallantoic membrane
by: Mohammed, M.H., et al.
Published: (2012)

Sequence and phylogenetic analysis of the VP2 gene of very virulent infectious bursal disease virus isolates
by: Hoque, Mahfuzul M., et al.
Published: (2002)

Development of in situ pcr technique for detection of velogenic newcastle disease virus strain
by: Elawad, H. A., et al.
Published: (2013)

In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens
by: Hussein, Elawad A., et al.
Published: (2017)

Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.
by: Omar, Abdul Rahman, et al.
Published: (2012)

High-level production of VP2 recombinant protein of very virulent infectious bursal disease virus in Escherichia coli system.
by: Bejo, Mohd Hair, et al.
Published: (2008)

Characterization of chicken splenic-derived dendritic cells following vaccine and very virulent strains of infectious bursal disease virus infection
by: Yasmin, A. R., et al.
Published: (2016)

Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken
by: Hussein, Elawad A., et al.
Published: (2020)

Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection
by: Mohd Isa, Nor Farah, et al.
Published: (2013)

Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection
by: Mohd Isa, Noor Farhanah, et al.
Published: (2012)

Differential activation of intraepithelial lymphocyte-natural killer cells in chickens infected with very virulent and vaccine strains of infectious bursal disease virus
by: Jahromi, Mohammad Zareian, et al.
Published: (2018)

Bursal immunopathology responses of specific-pathogen-free chickens and red jungle fowl infected with very virulent infectious bursal disease virus
by: Omar, Abdul Rahman, et al.
Published: (2018)

Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
by: Omar, Abdul Rahman, et al.
Published: (2010)

Pathogenicity and immunogenicity of infectious bursal disease virus in poultry
by: Bejo, Mohd Hair, et al.
Published: (1999)

Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR
by: Mahmoud, Hussein Abdallah Elawad, et al.
Published: (2015)

Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus
by: Rasoli, Mehdi, et al.
Published: (2015)

Pathogenicity And Immunogenicity Of Egg-Adapted Very Virulent Infectious Bursal Disease Virus Isolated In Malaysia
by: Hashim, Thuzar Than @ Hafiza
Published: (2004)

Infection with very virulent infectious bursal disease virus causes infiltration of t cells into bursa of fabricius
by: Jamil, Aimi Zulfadli, et al.
Published: (2013)

Cytokines and immune related gene expression alteration in chicken HD11 cell line following very virulent infectious bursal disease virus infection
by: Rasoli, Mehdi, et al.
Published: (2013)

Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus
by: Omar, Abdul Rahman, et al.
Published: (2006)

Comparison of Sybr Green I, ELISA and conventional agarose gel-based PCR in the detection of infectious bursal disease virus
by: Hamzah, Hairul Aini, et al.
Published: (2008)

Base Usage and Dinucleotide Frequency of Infectious Bursal Disease Virus
by: Tan, Do Yew, et al.
Published: (2004)

Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains
by: Ghazali, Mohd. Shahrin, et al.
Published: (2013)

Adaptation And Attenuation Of Very Virulent Infectious Bursal Disease Virus In Avian And Mammalian Cell Cultures For Vaccine Development
by: Khor, Sok Fang
Published: (2007)

Immunostimulatory effects of arginine in broiler chickens challenged with vaccine strain of infectious bursal disease virus
by: Emadi, Mozhdeh, et al.
Published: (2010)

Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
by: Hamzah, Hairul Aini, et al.
Published: (2004)

Molecular Characterisation, Attenuation and Inactivation of Very Virulent Infectious Bursal Disease Virus for the Development of Tissue Culture Based-Vaccines
by: Mohammed, Majed H.
Published: (2010)

Molecular Characterisation of Very Virulent Infectious Bursal Disease Virus and the Effects of Its Viral Proteins on Cancer Cell Lines
by: Voon, Kenny Gah Leong
Published: (2005)

Identifcation of reference genes in chicken intraepithelial lymphocyte natural killer cells infected with very-virulent infectious bursal disease virus
by: Boo, Sook Yee, et al.
Published: (2020)

Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus
by: Mohd Isa, Farhanah, et al.
Published: (2020)

Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
by: M.A., Bahmaninejad, et al.
Published: (2008)

Expression of segment a gene of UPM04/190 infectious bursal disease virus in Arabidopsis thaliana
by: Liew, Pit Sze, et al.
Published: (2015)