Adaptation of infectious bronchitis virus in mammalian cell line
Infectious bronchitis virus (IBV) is the causative agent of chicken infectious bronchitis (IB), an acute, highly contagious viral respiratory, urinary and reproductive tracts disease. Two different IBV inoculums (UPM1212) preparation was used in the study following inoculation of the virus into Afri...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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Faculty of Veterinary Medicine, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41394/ http://psasir.upm.edu.my/id/eprint/41394/1/41394.pdf |
| _version_ | 1848849683446759424 |
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| author | M. J., Suhairoslinda Hayati Bejo, Mohd Hair Arshad, Siti Suri Omar, Abdul Rahman |
| author_facet | M. J., Suhairoslinda Hayati Bejo, Mohd Hair Arshad, Siti Suri Omar, Abdul Rahman |
| author_sort | M. J., Suhairoslinda Hayati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Infectious bronchitis virus (IBV) is the causative agent of chicken infectious bronchitis (IB), an acute, highly contagious viral respiratory, urinary and reproductive tracts disease. Two different IBV inoculums (UPM1212) preparation was used in the study following inoculation of the virus into African green monkey kidney cell line (Vero cells) up to three consecutive passages. The study showed that the inoculums prepared directly from kidney of IBV infected chicken showed cytophatic effect (CPE) starting from the second (P2) to third passages (P3). The CPE was characterised by formation of syncytium, giant cell, dendritic shaped cell and finally plaque formation. The polymerase chain reaction (PCR) results confirmed the presence of IBV in the Vero cells at P2 and P3. In contrast, no CPE was observed in Vero cells inoculated with inoculums prepared from allantoic fluid following inoculation of the sample into specific pathogen free (SPF) embryonated chicken eggs. The PCR results were negative for IBV. |
| first_indexed | 2025-11-15T09:54:17Z |
| format | Conference or Workshop Item |
| id | upm-41394 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:54:17Z |
| publishDate | 2013 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-413942016-01-27T01:29:16Z http://psasir.upm.edu.my/id/eprint/41394/ Adaptation of infectious bronchitis virus in mammalian cell line M. J., Suhairoslinda Hayati Bejo, Mohd Hair Arshad, Siti Suri Omar, Abdul Rahman Infectious bronchitis virus (IBV) is the causative agent of chicken infectious bronchitis (IB), an acute, highly contagious viral respiratory, urinary and reproductive tracts disease. Two different IBV inoculums (UPM1212) preparation was used in the study following inoculation of the virus into African green monkey kidney cell line (Vero cells) up to three consecutive passages. The study showed that the inoculums prepared directly from kidney of IBV infected chicken showed cytophatic effect (CPE) starting from the second (P2) to third passages (P3). The CPE was characterised by formation of syncytium, giant cell, dendritic shaped cell and finally plaque formation. The polymerase chain reaction (PCR) results confirmed the presence of IBV in the Vero cells at P2 and P3. In contrast, no CPE was observed in Vero cells inoculated with inoculums prepared from allantoic fluid following inoculation of the sample into specific pathogen free (SPF) embryonated chicken eggs. The PCR results were negative for IBV. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41394/1/41394.pdf M. J., Suhairoslinda Hayati and Bejo, Mohd Hair and Arshad, Siti Suri and Omar, Abdul Rahman (2013) Adaptation of infectious bronchitis virus in mammalian cell line. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 92-94). http://www.vet.upm.edu.my/dokumen/90301_proceeding_WPSA_V2_first_second_XX_new_20121013_%281%29.pdf |
| spellingShingle | M. J., Suhairoslinda Hayati Bejo, Mohd Hair Arshad, Siti Suri Omar, Abdul Rahman Adaptation of infectious bronchitis virus in mammalian cell line |
| title | Adaptation of infectious bronchitis virus in mammalian cell line |
| title_full | Adaptation of infectious bronchitis virus in mammalian cell line |
| title_fullStr | Adaptation of infectious bronchitis virus in mammalian cell line |
| title_full_unstemmed | Adaptation of infectious bronchitis virus in mammalian cell line |
| title_short | Adaptation of infectious bronchitis virus in mammalian cell line |
| title_sort | adaptation of infectious bronchitis virus in mammalian cell line |
| url | http://psasir.upm.edu.my/id/eprint/41394/ http://psasir.upm.edu.my/id/eprint/41394/ http://psasir.upm.edu.my/id/eprint/41394/1/41394.pdf |