Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231)
Introduction: Curcumin, a natural compound present in turmeric (Curcuma longa) has been known to possess both anti-inflammatory and antioxidant effects. Objectives: The objectives of the study were to evaluate the cytotoxic activities and to determine the mode of cell death induced by curcumin towar...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2006
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/41185/ http://psasir.upm.edu.my/id/eprint/41185/1/Curcumin%20from%20turmeric%20%28Curcuma%20longa%29%20induced%20apoptosis%20in%20human%20mammary%20carcinoma%20cells%20%28MDA-MB-231%29.pdf |
| _version_ | 1848849627750596608 |
|---|---|
| author | Saiful Yazan, Latifah Ahmad, Faujan Lee, Pei Sze Abdul Rahim, Raha Abdul Hamid, Hisyam Ooi, Choong Li |
| author_facet | Saiful Yazan, Latifah Ahmad, Faujan Lee, Pei Sze Abdul Rahim, Raha Abdul Hamid, Hisyam Ooi, Choong Li |
| author_sort | Saiful Yazan, Latifah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: Curcumin, a natural compound present in turmeric (Curcuma longa) has been known to possess both anti-inflammatory and antioxidant effects. Objectives: The objectives of the study were to evaluate the cytotoxic activities and to determine the mode of cell death induced by curcumin towards the human mammary carcinoma cells (MDAMB-231). Methodology: Cytotoxicity of curcumin and its effect on cell viability were determined by using MTT assay and trypan blue dye exclusion method, respectively. The mode of cell death was detected by viewing under a light microscope and through DNA fragmentation analysis. Results and discussion: Curcumin was cytotoxic to MDA-MB-231 cells with the IC50 of 17.25 ì g/ml. Cell viability treatment using curcumin at concentrations of 30 ì g/ml and 10 ì g/ml was significantly (p<0.05) reduced at 48 and 56 hours, respectively, compared to the control. Cells treated with curcumin (30, 10, 3, and 1ì g/ml) showed apoptotic features such as shrinkage of cell, membrane blebbing, chromatin condensation and nuclear fragmentation under a light microscope. DNA extracted from cells treated with curcumin at all concentrations were intact after 24 hours but fragmented after 72 hours, forming DNA laddering on agarose gel, a hallmark of apoptosis. Conclusion: Induction of apoptosis in breast cancer cells by curcumin suggests its potential use as a strategy for cancer control. |
| first_indexed | 2025-11-15T09:53:24Z |
| format | Article |
| id | upm-41185 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:53:24Z |
| publishDate | 2006 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-411852015-12-07T01:14:16Z http://psasir.upm.edu.my/id/eprint/41185/ Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231) Saiful Yazan, Latifah Ahmad, Faujan Lee, Pei Sze Abdul Rahim, Raha Abdul Hamid, Hisyam Ooi, Choong Li Introduction: Curcumin, a natural compound present in turmeric (Curcuma longa) has been known to possess both anti-inflammatory and antioxidant effects. Objectives: The objectives of the study were to evaluate the cytotoxic activities and to determine the mode of cell death induced by curcumin towards the human mammary carcinoma cells (MDAMB-231). Methodology: Cytotoxicity of curcumin and its effect on cell viability were determined by using MTT assay and trypan blue dye exclusion method, respectively. The mode of cell death was detected by viewing under a light microscope and through DNA fragmentation analysis. Results and discussion: Curcumin was cytotoxic to MDA-MB-231 cells with the IC50 of 17.25 ì g/ml. Cell viability treatment using curcumin at concentrations of 30 ì g/ml and 10 ì g/ml was significantly (p<0.05) reduced at 48 and 56 hours, respectively, compared to the control. Cells treated with curcumin (30, 10, 3, and 1ì g/ml) showed apoptotic features such as shrinkage of cell, membrane blebbing, chromatin condensation and nuclear fragmentation under a light microscope. DNA extracted from cells treated with curcumin at all concentrations were intact after 24 hours but fragmented after 72 hours, forming DNA laddering on agarose gel, a hallmark of apoptosis. Conclusion: Induction of apoptosis in breast cancer cells by curcumin suggests its potential use as a strategy for cancer control. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2006-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41185/1/Curcumin%20from%20turmeric%20%28Curcuma%20longa%29%20induced%20apoptosis%20in%20human%20mammary%20carcinoma%20cells%20%28MDA-MB-231%29.pdf Saiful Yazan, Latifah and Ahmad, Faujan and Lee, Pei Sze and Abdul Rahim, Raha and Abdul Hamid, Hisyam and Ooi, Choong Li (2006) Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231). Malaysian Journal of Medicine and Health Sciences, 2 (2). pp. 71-79. ISSN 1675-8544 http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2006V02N2_OP03.pdf |
| spellingShingle | Saiful Yazan, Latifah Ahmad, Faujan Lee, Pei Sze Abdul Rahim, Raha Abdul Hamid, Hisyam Ooi, Choong Li Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231) |
| title | Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231) |
| title_full | Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231) |
| title_fullStr | Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231) |
| title_full_unstemmed | Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231) |
| title_short | Curcumin from turmeric (Curcuma longa) induced apoptosis in human mammary carcinoma cells (MDA-MB-231) |
| title_sort | curcumin from turmeric (curcuma longa) induced apoptosis in human mammary carcinoma cells (mda-mb-231) |
| url | http://psasir.upm.edu.my/id/eprint/41185/ http://psasir.upm.edu.my/id/eprint/41185/ http://psasir.upm.edu.my/id/eprint/41185/1/Curcumin%20from%20turmeric%20%28Curcuma%20longa%29%20induced%20apoptosis%20in%20human%20mammary%20carcinoma%20cells%20%28MDA-MB-231%29.pdf |