Screening for thalassaemia in pregnant women: a laboratory perspective
Aim: To investigate whether in Malaysia, a mean corpuscular volume (MCV) less than 80 f1 and mean corpuscular haemoglobin (MCH) less than 27 pg will identify carriers in pregnant women with severe forms of thalassaemia, α-thal 1 (α°) and classical β (β°)- thalassaemia. The results from this study wi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41145/ http://psasir.upm.edu.my/id/eprint/41145/1/Screening%20for%20thalassaemia%20in%20pregnant%20women%20a%20laboratory%20perspective.pdf |
| _version_ | 1848849616783540224 |
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| author | George, Elizabeth Khoo, S. K. Abu Bakar, Mokhtar Umar, Nor Aini |
| author_facet | George, Elizabeth Khoo, S. K. Abu Bakar, Mokhtar Umar, Nor Aini |
| author_sort | George, Elizabeth |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aim: To investigate whether in Malaysia, a mean corpuscular volume (MCV) less than 80 f1 and mean corpuscular haemoglobin (MCH) less than 27 pg will identify carriers in pregnant women with severe forms of thalassaemia, α-thal 1 (α°) and classical β (β°)- thalassaemia. The results from this study will aid the implementation of a national program to screen for thalassaemia. Methods: For classical β (β°)- thalassaemia, blood samples collected in EDTA from 153 pregnant women were taken for full blood counts and haemoglobin subtyping by automated blood counting and high performance liquid chromatography (HPLC) respectively. For α-thal 1 (α°), the full blood counts were obtained from archives of 30 pregnant women who were genotyped positive for the α-thal 1 (α°) during prenatal diagnosis for Hb Barts hydrops fetalis. The effects of storage on MCV, MCH and Hb A₂ were determined by tests done daily for 3 weeks. Results: By correlating red cell indices with high performance liquid chromatography and genotypes data, we show that mean corpuscular volume (MCV) <80f1 and mean corpuscular haemoglobin <27pg is able to detect all heterozygous carriers with classical β (β°)- thalassaemia. On storage, MCV of heterozygous carriers with classical β (β°)- thalassaemia rose at 1% a day after 24 hours reaching a mean of 80 f1 by day 15. However, the MCH and Hb A₂ were stable for 3 weeks. Conclusion: A mean corpuscular haemoglobin <27pg should be recommended as cut-off values for screening of carries of α-thal 1 (α°) and classical β (β°)- thalassaemia. In blood samples, not processed within a day, MCH with a cut-off 27pg is the recommended choice for screening of carriers. |
| first_indexed | 2025-11-15T09:53:14Z |
| format | Article |
| id | upm-41145 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:53:14Z |
| publishDate | 2005 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-411452015-12-03T04:59:55Z http://psasir.upm.edu.my/id/eprint/41145/ Screening for thalassaemia in pregnant women: a laboratory perspective George, Elizabeth Khoo, S. K. Abu Bakar, Mokhtar Umar, Nor Aini Aim: To investigate whether in Malaysia, a mean corpuscular volume (MCV) less than 80 f1 and mean corpuscular haemoglobin (MCH) less than 27 pg will identify carriers in pregnant women with severe forms of thalassaemia, α-thal 1 (α°) and classical β (β°)- thalassaemia. The results from this study will aid the implementation of a national program to screen for thalassaemia. Methods: For classical β (β°)- thalassaemia, blood samples collected in EDTA from 153 pregnant women were taken for full blood counts and haemoglobin subtyping by automated blood counting and high performance liquid chromatography (HPLC) respectively. For α-thal 1 (α°), the full blood counts were obtained from archives of 30 pregnant women who were genotyped positive for the α-thal 1 (α°) during prenatal diagnosis for Hb Barts hydrops fetalis. The effects of storage on MCV, MCH and Hb A₂ were determined by tests done daily for 3 weeks. Results: By correlating red cell indices with high performance liquid chromatography and genotypes data, we show that mean corpuscular volume (MCV) <80f1 and mean corpuscular haemoglobin <27pg is able to detect all heterozygous carriers with classical β (β°)- thalassaemia. On storage, MCV of heterozygous carriers with classical β (β°)- thalassaemia rose at 1% a day after 24 hours reaching a mean of 80 f1 by day 15. However, the MCH and Hb A₂ were stable for 3 weeks. Conclusion: A mean corpuscular haemoglobin <27pg should be recommended as cut-off values for screening of carries of α-thal 1 (α°) and classical β (β°)- thalassaemia. In blood samples, not processed within a day, MCH with a cut-off 27pg is the recommended choice for screening of carriers. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2005-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41145/1/Screening%20for%20thalassaemia%20in%20pregnant%20women%20a%20laboratory%20perspective.pdf George, Elizabeth and Khoo, S. K. and Abu Bakar, Mokhtar and Umar, Nor Aini (2005) Screening for thalassaemia in pregnant women: a laboratory perspective. Malaysian Journal of Medicine and Health Sciences, 1 (2). pp. 111-117. ISSN 1675-8544 http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2005V01N2_OP04.pdf |
| spellingShingle | George, Elizabeth Khoo, S. K. Abu Bakar, Mokhtar Umar, Nor Aini Screening for thalassaemia in pregnant women: a laboratory perspective |
| title | Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_full | Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_fullStr | Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_full_unstemmed | Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_short | Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_sort | screening for thalassaemia in pregnant women: a laboratory perspective |
| url | http://psasir.upm.edu.my/id/eprint/41145/ http://psasir.upm.edu.my/id/eprint/41145/ http://psasir.upm.edu.my/id/eprint/41145/1/Screening%20for%20thalassaemia%20in%20pregnant%20women%20a%20laboratory%20perspective.pdf |