Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates
Mycoplasma gallisepticum (MG), an agent that causes chronic respiratory diseases in the avian, possesses a cytadhesin mgc2 gene. Mgc2 is a second cytadhesin-like protein, localised on the tenninal bleb involved in the attachment of MG to host tissue. Five reference MG strains (S6, R, F, TSll and 6/8...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary Association Malaysia
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/41085/ http://psasir.upm.edu.my/id/eprint/41085/1/Molecular%20characterisation%20of%20mycoplasma%20gallisepticum%20MGC2%20gene%20from%20commercial%20chicken%20isolates.pdf |
| _version_ | 1848849600695238656 |
|---|---|
| author | Ahmad, Kartini Ideris, Aini Omar, Abdul Rahman Tan, C. G. |
| author_facet | Ahmad, Kartini Ideris, Aini Omar, Abdul Rahman Tan, C. G. |
| author_sort | Ahmad, Kartini |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mycoplasma gallisepticum (MG), an agent that causes chronic respiratory diseases in the avian, possesses a cytadhesin mgc2 gene. Mgc2 is a second cytadhesin-like protein, localised on the tenninal bleb involved in the attachment of MG to host tissue. Five reference MG strains (S6, R, F, TSll and 6/85) and 18 field isolates were extracted, sequenced and analysed, including another 30 published isolate sequences from V.S., Australia and Israel.
The mgc2 gene isolates exhibit high G + C base content of 45% due to the presence of high proline (14 to 16%) and glycine (13 to 14%) residues located at the two-third position of the carboxy terminal region. The Malaysian field isolates were divided into four categories: (i) 854 - 857 bp amplicon, (ii) 837 bp amplicon, (iii) 822 - 824 bp amplicon, and (iv) 791 bp amplicon, due to gene size polymorphism. Six field isolates (KPR44 L, KPR16W 44 L, THNG8W L, AK2 VC, PF3H Br and PF7V Br) exhibited several mutations at the 3' region located at positions 166, 173, 195 and 202 a.a., which distinctively differ from other reference strains but appear identical to Israeli isolates. Other field isolates and certain published sequences were either totally similar or almost similar to MGS6, TS lland 6/85 strains. |
| first_indexed | 2025-11-15T09:52:59Z |
| format | Article |
| id | upm-41085 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:52:59Z |
| publishDate | 2011 |
| publisher | Veterinary Association Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-410852015-12-02T04:37:50Z http://psasir.upm.edu.my/id/eprint/41085/ Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates Ahmad, Kartini Ideris, Aini Omar, Abdul Rahman Tan, C. G. Mycoplasma gallisepticum (MG), an agent that causes chronic respiratory diseases in the avian, possesses a cytadhesin mgc2 gene. Mgc2 is a second cytadhesin-like protein, localised on the tenninal bleb involved in the attachment of MG to host tissue. Five reference MG strains (S6, R, F, TSll and 6/85) and 18 field isolates were extracted, sequenced and analysed, including another 30 published isolate sequences from V.S., Australia and Israel. The mgc2 gene isolates exhibit high G + C base content of 45% due to the presence of high proline (14 to 16%) and glycine (13 to 14%) residues located at the two-third position of the carboxy terminal region. The Malaysian field isolates were divided into four categories: (i) 854 - 857 bp amplicon, (ii) 837 bp amplicon, (iii) 822 - 824 bp amplicon, and (iv) 791 bp amplicon, due to gene size polymorphism. Six field isolates (KPR44 L, KPR16W 44 L, THNG8W L, AK2 VC, PF3H Br and PF7V Br) exhibited several mutations at the 3' region located at positions 166, 173, 195 and 202 a.a., which distinctively differ from other reference strains but appear identical to Israeli isolates. Other field isolates and certain published sequences were either totally similar or almost similar to MGS6, TS lland 6/85 strains. Veterinary Association Malaysia 2011-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41085/1/Molecular%20characterisation%20of%20mycoplasma%20gallisepticum%20MGC2%20gene%20from%20commercial%20chicken%20isolates.pdf Ahmad, Kartini and Ideris, Aini and Omar, Abdul Rahman and Tan, C. G. (2011) Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates. Jurnal Veterinar Malaysia, 23 (1). pp. 11-18. ISSN 9128-2506 http://vam.org.my/home/2014/06/jvm-2011-july-vol-23-no-1/ |
| spellingShingle | Ahmad, Kartini Ideris, Aini Omar, Abdul Rahman Tan, C. G. Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates |
| title | Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates |
| title_full | Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates |
| title_fullStr | Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates |
| title_full_unstemmed | Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates |
| title_short | Molecular characterisation of Mycoplasma gallisepticum MGC2 gene from commercial chicken isolates |
| title_sort | molecular characterisation of mycoplasma gallisepticum mgc2 gene from commercial chicken isolates |
| url | http://psasir.upm.edu.my/id/eprint/41085/ http://psasir.upm.edu.my/id/eprint/41085/ http://psasir.upm.edu.my/id/eprint/41085/1/Molecular%20characterisation%20of%20mycoplasma%20gallisepticum%20MGC2%20gene%20from%20commercial%20chicken%20isolates.pdf |