Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces
This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber, mango and guava surface, as well as to determine the relationship between time contact and biofilm formation. Crystal violet assay was performed to quantify the biofilm formation based on the value of optica...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41050/ http://psasir.upm.edu.my/id/eprint/41050/1/Biofilm%20forming%20ability%20and%20time%20course%20study%20of%20growth%20of%20Salmonella%20Typhi%20on%20fresh%20produce%20surfaces.pdf |
| _version_ | 1848849591562141696 |
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| author | Tang, Poh Li Pui, Chai Fung Wong, Woan Chwen Ahmad, Noorlis Radu, Son |
| author_facet | Tang, Poh Li Pui, Chai Fung Wong, Woan Chwen Ahmad, Noorlis Radu, Son |
| author_sort | Tang, Poh Li |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber, mango and guava surface, as well as to determine the relationship between time contact and biofilm formation. Crystal violet assay was performed to quantify the biofilm formation based on the value of optical density at 570 nm of the destaining crystal violet at the specific interval time. The result showed that the attachment of the bacterial cells on the fresh produce surface increased with the contact time. The readings of OD 570 at time 12 h for cucumber, mango and guava surfaces were 0.824, 0.683 and 0.598, respectively, indicating that the biofilm formation by Salmonella Typhi on different fresh produce surface varied with time. Since the result showed that Salmonella Typhi formed biofilm on fresh produce surfaces, hygienic practice from farm to fork including handling, processing, distribution and storage of the fresh produce should be of concern. |
| first_indexed | 2025-11-15T09:52:50Z |
| format | Article |
| id | upm-41050 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:52:50Z |
| publishDate | 2012 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-410502015-11-30T07:56:52Z http://psasir.upm.edu.my/id/eprint/41050/ Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces Tang, Poh Li Pui, Chai Fung Wong, Woan Chwen Ahmad, Noorlis Radu, Son This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber, mango and guava surface, as well as to determine the relationship between time contact and biofilm formation. Crystal violet assay was performed to quantify the biofilm formation based on the value of optical density at 570 nm of the destaining crystal violet at the specific interval time. The result showed that the attachment of the bacterial cells on the fresh produce surface increased with the contact time. The readings of OD 570 at time 12 h for cucumber, mango and guava surfaces were 0.824, 0.683 and 0.598, respectively, indicating that the biofilm formation by Salmonella Typhi on different fresh produce surface varied with time. Since the result showed that Salmonella Typhi formed biofilm on fresh produce surfaces, hygienic practice from farm to fork including handling, processing, distribution and storage of the fresh produce should be of concern. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41050/1/Biofilm%20forming%20ability%20and%20time%20course%20study%20of%20growth%20of%20Salmonella%20Typhi%20on%20fresh%20produce%20surfaces.pdf Tang, Poh Li and Pui, Chai Fung and Wong, Woan Chwen and Ahmad, Noorlis and Radu, Son (2012) Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces. International Food Research Journal, 19 (1). pp. 71-76. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/19%20%2801%29%202011/%2810%29IFRJ-2011-276%20Son.pdf |
| spellingShingle | Tang, Poh Li Pui, Chai Fung Wong, Woan Chwen Ahmad, Noorlis Radu, Son Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_full | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_fullStr | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_full_unstemmed | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_short | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_sort | biofilm forming ability and time course study of growth of salmonella typhi on fresh produce surfaces |
| url | http://psasir.upm.edu.my/id/eprint/41050/ http://psasir.upm.edu.my/id/eprint/41050/ http://psasir.upm.edu.my/id/eprint/41050/1/Biofilm%20forming%20ability%20and%20time%20course%20study%20of%20growth%20of%20Salmonella%20Typhi%20on%20fresh%20produce%20surfaces.pdf |