Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia
A total of 32 clinical strains of Vibrio cholerae, including members of the 01 and 0139 serogroup were collected from Klang, Selangor; Penang Island; Samarahan, Sarawak and Miri, Sarawak in Malaysia. In general, all the isolates except the 0139 serotype expressed low resistance to all the antibiotic...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41015/ http://psasir.upm.edu.my/id/eprint/41015/1/Molecular%20characterization%20of%20clinical%20isolate%20of%20Vibrio%20cholera%20isolated%20from%20outbreaks%20cases%20in%20Malaysia%20%28p1267-p1274%29.pdf |
| _version_ | 1848849583789047808 |
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| author | Patrick, Guda Benjamin Nishibuchi, Mitsuaki Robin, Tunung Radu, Son |
| author_facet | Patrick, Guda Benjamin Nishibuchi, Mitsuaki Robin, Tunung Radu, Son |
| author_sort | Patrick, Guda Benjamin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A total of 32 clinical strains of Vibrio cholerae, including members of the 01 and 0139 serogroup were collected from Klang, Selangor; Penang Island; Samarahan, Sarawak and Miri, Sarawak in Malaysia. In general, all the isolates except the 0139 serotype expressed low resistance to all the antibiotics tested with their Multiple Antibiotic Resistance (MAR) indices ranged from 0.10 to 0.48. The presence of ctx gene that encoded the cholera toxin was confirmed in all these clinical isolates by polymerase chain reaction. The results from the RAPD-PCR were analyzed using the RAPDistance software (Version 1.04). From the dendrogram generated, two main groups were observed which were subdivided into two clusters each. The Selangor's isolates and the 0139 Penang's isolates formed one group whereas the Samarahan, Sarawak isolates and the Miri, Sarawak isolates made up the other group, thus delineating their different sources of origin based on their geographical location. |
| first_indexed | 2025-11-15T09:52:42Z |
| format | Article |
| id | upm-41015 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:52:42Z |
| publishDate | 2012 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-410152015-11-30T04:52:17Z http://psasir.upm.edu.my/id/eprint/41015/ Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia Patrick, Guda Benjamin Nishibuchi, Mitsuaki Robin, Tunung Radu, Son A total of 32 clinical strains of Vibrio cholerae, including members of the 01 and 0139 serogroup were collected from Klang, Selangor; Penang Island; Samarahan, Sarawak and Miri, Sarawak in Malaysia. In general, all the isolates except the 0139 serotype expressed low resistance to all the antibiotics tested with their Multiple Antibiotic Resistance (MAR) indices ranged from 0.10 to 0.48. The presence of ctx gene that encoded the cholera toxin was confirmed in all these clinical isolates by polymerase chain reaction. The results from the RAPD-PCR were analyzed using the RAPDistance software (Version 1.04). From the dendrogram generated, two main groups were observed which were subdivided into two clusters each. The Selangor's isolates and the 0139 Penang's isolates formed one group whereas the Samarahan, Sarawak isolates and the Miri, Sarawak isolates made up the other group, thus delineating their different sources of origin based on their geographical location. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41015/1/Molecular%20characterization%20of%20clinical%20isolate%20of%20Vibrio%20cholera%20isolated%20from%20outbreaks%20cases%20in%20Malaysia%20%28p1267-p1274%29.pdf Patrick, Guda Benjamin and Nishibuchi, Mitsuaki and Robin, Tunung and Radu, Son (2012) Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia. International Food Research Journal, 19 (3). pp. 1267-1274. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/19%20%2803%29%202012/%2866%29%20IFRJ%2019%20%2803%29%202012%20Patrick.pdf |
| spellingShingle | Patrick, Guda Benjamin Nishibuchi, Mitsuaki Robin, Tunung Radu, Son Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia |
| title | Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia |
| title_full | Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia |
| title_fullStr | Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia |
| title_full_unstemmed | Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia |
| title_short | Molecular characterization of clinical isolate of Vibrio cholera isolated from outbreaks cases in Malaysia |
| title_sort | molecular characterization of clinical isolate of vibrio cholera isolated from outbreaks cases in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/41015/ http://psasir.upm.edu.my/id/eprint/41015/ http://psasir.upm.edu.my/id/eprint/41015/1/Molecular%20characterization%20of%20clinical%20isolate%20of%20Vibrio%20cholera%20isolated%20from%20outbreaks%20cases%20in%20Malaysia%20%28p1267-p1274%29.pdf |