Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism
The objective of the present study was to develop a rapid, reliable and yet inexpensive protocol for genomic DNA extraction from frozen and ethanol-preserved Asian green-lipped mussels for random amplified microsatelite (RAM) analysis. The procedure comprised of three major steps: (1) Tissue degrada...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40902/ http://psasir.upm.edu.my/id/eprint/40902/1/15%20IFRJ-2008-140%20Chai%20Malaysia%20FINAL.pdf |
| _version_ | 1848849552259416064 |
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| author | Chai, Lay Ching Abdullah, Fatimah Corazon Mat Sout, Norhisyam Alias, Rozila Abu Samah, Nadzirah Lee, Natasha Hai Yen |
| author_facet | Chai, Lay Ching Abdullah, Fatimah Corazon Mat Sout, Norhisyam Alias, Rozila Abu Samah, Nadzirah Lee, Natasha Hai Yen |
| author_sort | Chai, Lay Ching |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objective of the present study was to develop a rapid, reliable and yet inexpensive protocol for genomic DNA extraction from frozen and ethanol-preserved Asian green-lipped mussels for random amplified microsatelite (RAM) analysis. The procedure comprised of three major steps: (1) Tissue degradation by boiling in 6% Chelex 100 resin in TE buffer; (2) Protein digestion by Proteinase K; and (3) DNA precipitation by adding 2 volumes of cold absolute ethanol. The entire procedure can be completed within two hours. The resulting RAM profiles were clear and reproducible. Our results demonstrate that the combined protocol of Chelex 100- Proteinase K-ethanol precipitation is a powerful yet economical DNA isolation method for population genetic studies involving a large sample size. |
| first_indexed | 2025-11-15T09:52:12Z |
| format | Article |
| id | upm-40902 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:52:12Z |
| publishDate | 2009 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-409022015-10-09T05:02:05Z http://psasir.upm.edu.my/id/eprint/40902/ Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism Chai, Lay Ching Abdullah, Fatimah Corazon Mat Sout, Norhisyam Alias, Rozila Abu Samah, Nadzirah Lee, Natasha Hai Yen The objective of the present study was to develop a rapid, reliable and yet inexpensive protocol for genomic DNA extraction from frozen and ethanol-preserved Asian green-lipped mussels for random amplified microsatelite (RAM) analysis. The procedure comprised of three major steps: (1) Tissue degradation by boiling in 6% Chelex 100 resin in TE buffer; (2) Protein digestion by Proteinase K; and (3) DNA precipitation by adding 2 volumes of cold absolute ethanol. The entire procedure can be completed within two hours. The resulting RAM profiles were clear and reproducible. Our results demonstrate that the combined protocol of Chelex 100- Proteinase K-ethanol precipitation is a powerful yet economical DNA isolation method for population genetic studies involving a large sample size. Faculty of Food Science and Technology, Universiti Putra Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40902/1/15%20IFRJ-2008-140%20Chai%20Malaysia%20FINAL.pdf Chai, Lay Ching and Abdullah, Fatimah Corazon and Mat Sout, Norhisyam and Alias, Rozila and Abu Samah, Nadzirah and Lee, Natasha Hai Yen (2009) Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism. International Food Research Journal, 16 (1). pp. 113-118. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/16%20%281%29%202009/15%20IFRJ-2008-140%20Chai%20Malaysia%20FINAL.pdf |
| spellingShingle | Chai, Lay Ching Abdullah, Fatimah Corazon Mat Sout, Norhisyam Alias, Rozila Abu Samah, Nadzirah Lee, Natasha Hai Yen Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism |
| title | Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism |
| title_full | Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism |
| title_fullStr | Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism |
| title_full_unstemmed | Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism |
| title_short | Rapid isolation of genomic DNA from Asian green-lipped mussel (Perna viridis) for random amplified microsatellite polymorphism |
| title_sort | rapid isolation of genomic dna from asian green-lipped mussel (perna viridis) for random amplified microsatellite polymorphism |
| url | http://psasir.upm.edu.my/id/eprint/40902/ http://psasir.upm.edu.my/id/eprint/40902/ http://psasir.upm.edu.my/id/eprint/40902/1/15%20IFRJ-2008-140%20Chai%20Malaysia%20FINAL.pdf |