Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR
Pathogenic Vibrio parahaemolyticus is one of the leading causes of bacterial gastroenteritis in many countries. Among the strains examined, 36 RAPD-types were found when amplified with primers OPA8 and OPA10. The analysis shows the majority of V. parahaemolyticus isolates originated from seafood wer...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40753/ http://psasir.upm.edu.my/id/eprint/40753/1/8%20IFRJ%2021%20%2801%29%202014%20Alex%20571.pdf |
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| author | Nillian, Elexson Rukayadi, Yaya Mahyudin, Nor Ainy Kantilal, Haresh Kumar Anyi, Ubong Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_facet | Nillian, Elexson Rukayadi, Yaya Mahyudin, Nor Ainy Kantilal, Haresh Kumar Anyi, Ubong Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_sort | Nillian, Elexson |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pathogenic Vibrio parahaemolyticus is one of the leading causes of bacterial gastroenteritis in many countries. Among the strains examined, 36 RAPD-types were found when amplified with primers OPA8 and OPA10. The analysis shows the majority of V. parahaemolyticus isolates originated from seafood were branched into four major clusters at 18.2%, 20.7% 34% and 3.4% similarity levels. This suggests that there is potential for a single strain to be distributed widely within a population and there also potential for multiple contaminating strains of different clonal lineages to be present within the same population. Optimum temperature (37°C) was the highest and stable formation of biofilm. The total percentage of biofilm formation at 37°C was 33.33% for each of weak, moderate and strong biofilm producers. Room temperature produces 61.1% of weak biofilm producer, while 13. 89% for moderate biofilm producers and produce 25% of strong biofilm. While a total of 91.67% weak biofilm producers at 4°C and 8:33% for room temperature and no growth of strong biofilm. Upon analysis, strong biofilm was tracked from the largest group at 37°C and room temperature which produce 27.27% of strong biofilm producer respectively. Interestingly, they are derived from cockles. |
| first_indexed | 2025-11-15T09:51:36Z |
| format | Article |
| id | upm-40753 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:51:36Z |
| publishDate | 2014 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-407532019-11-22T00:53:58Z http://psasir.upm.edu.my/id/eprint/40753/ Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR Nillian, Elexson Rukayadi, Yaya Mahyudin, Nor Ainy Kantilal, Haresh Kumar Anyi, Ubong Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Pathogenic Vibrio parahaemolyticus is one of the leading causes of bacterial gastroenteritis in many countries. Among the strains examined, 36 RAPD-types were found when amplified with primers OPA8 and OPA10. The analysis shows the majority of V. parahaemolyticus isolates originated from seafood were branched into four major clusters at 18.2%, 20.7% 34% and 3.4% similarity levels. This suggests that there is potential for a single strain to be distributed widely within a population and there also potential for multiple contaminating strains of different clonal lineages to be present within the same population. Optimum temperature (37°C) was the highest and stable formation of biofilm. The total percentage of biofilm formation at 37°C was 33.33% for each of weak, moderate and strong biofilm producers. Room temperature produces 61.1% of weak biofilm producer, while 13. 89% for moderate biofilm producers and produce 25% of strong biofilm. While a total of 91.67% weak biofilm producers at 4°C and 8:33% for room temperature and no growth of strong biofilm. Upon analysis, strong biofilm was tracked from the largest group at 37°C and room temperature which produce 27.27% of strong biofilm producer respectively. Interestingly, they are derived from cockles. Faculty of Food Science and Technology, Universiti Putra Malaysia 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40753/1/8%20IFRJ%2021%20%2801%29%202014%20Alex%20571.pdf Nillian, Elexson and Rukayadi, Yaya and Mahyudin, Nor Ainy and Kantilal, Haresh Kumar and Anyi, Ubong and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2014) Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR. International Food Research Journal, 21 (1). pp. 59-65. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/21%20%2801%29%202014/8%20IFRJ%2021%20%2801%29%202014%20Alex%20571.pdf |
| spellingShingle | Nillian, Elexson Rukayadi, Yaya Mahyudin, Nor Ainy Kantilal, Haresh Kumar Anyi, Ubong Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR |
| title | Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR |
| title_full | Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR |
| title_fullStr | Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR |
| title_full_unstemmed | Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR |
| title_short | Biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism DNA-PCR |
| title_sort | biofilm assessment of vibrio parahaemolyticus from seafood using random amplified polymorphism dna-pcr |
| url | http://psasir.upm.edu.my/id/eprint/40753/ http://psasir.upm.edu.my/id/eprint/40753/ http://psasir.upm.edu.my/id/eprint/40753/1/8%20IFRJ%2021%20%2801%29%202014%20Alex%20571.pdf |