Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis
Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their surrounding environment were determined. The levels of resistance of the E. coli isolates towards six different antibiotics tested differed considerably. Though the isolates displayed resistance towar...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40682/ http://psasir.upm.edu.my/id/eprint/40682/1/Characterization%20of%20Escherichia%20coli%20isolated%20from%20cultured%20catfish%20by%20antibiotic%20resistance%20and%20RAPD%20analysis.pdf |
| _version_ | 1848849495645749248 |
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| author | Lihan, Samuel Mobilik, Marian Madonna Apun, Kasing Bilung, Lesley Maurice Radu, Son |
| author_facet | Lihan, Samuel Mobilik, Marian Madonna Apun, Kasing Bilung, Lesley Maurice Radu, Son |
| author_sort | Lihan, Samuel |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their surrounding environment were determined. The levels of resistance of the E. coli isolates towards six different antibiotics tested differed considerably. Though the isolates displayed resistance towards some of the antibiotics tested, none of the isolates showed resistant towards norfloxacin, sulphametoxazole/trimethoprim and chloramphenicol. RAPD-PCR analysis using single primer and primers combination clustered the E. coli isolates into 3 and 5 groups, respectively. The results of this study suggest that the E. coli isolates from the catfish and their surrounding environment derived from a mixture of sensitive and resistant strains with diverse genetic contents. The use of the RAPD analysis is sufficiently discriminatory for the typing of the E. coli isolates. |
| first_indexed | 2025-11-15T09:51:18Z |
| format | Article |
| id | upm-40682 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:51:18Z |
| publishDate | 2011 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-406822019-10-24T03:51:27Z http://psasir.upm.edu.my/id/eprint/40682/ Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis Lihan, Samuel Mobilik, Marian Madonna Apun, Kasing Bilung, Lesley Maurice Radu, Son Antibiotic susceptibility and genetic diversity of E. coli isolated from cultured catfish and their surrounding environment were determined. The levels of resistance of the E. coli isolates towards six different antibiotics tested differed considerably. Though the isolates displayed resistance towards some of the antibiotics tested, none of the isolates showed resistant towards norfloxacin, sulphametoxazole/trimethoprim and chloramphenicol. RAPD-PCR analysis using single primer and primers combination clustered the E. coli isolates into 3 and 5 groups, respectively. The results of this study suggest that the E. coli isolates from the catfish and their surrounding environment derived from a mixture of sensitive and resistant strains with diverse genetic contents. The use of the RAPD analysis is sufficiently discriminatory for the typing of the E. coli isolates. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40682/1/Characterization%20of%20Escherichia%20coli%20isolated%20from%20cultured%20catfish%20by%20antibiotic%20resistance%20and%20RAPD%20analysis.pdf Lihan, Samuel and Mobilik, Marian Madonna and Apun, Kasing and Bilung, Lesley Maurice and Radu, Son (2011) Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis. International Food Research Journal, 18 (3). pp. 971-976. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/18%20%2803%29%202011/%2816%29IFRJ-2010-089.pdf |
| spellingShingle | Lihan, Samuel Mobilik, Marian Madonna Apun, Kasing Bilung, Lesley Maurice Radu, Son Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
| title | Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
| title_full | Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
| title_fullStr | Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
| title_full_unstemmed | Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
| title_short | Characterization of Escherichia coli isolated from cultured catfish by antibiotic resistance and RAPD analysis |
| title_sort | characterization of escherichia coli isolated from cultured catfish by antibiotic resistance and rapd analysis |
| url | http://psasir.upm.edu.my/id/eprint/40682/ http://psasir.upm.edu.my/id/eprint/40682/ http://psasir.upm.edu.my/id/eprint/40682/1/Characterization%20of%20Escherichia%20coli%20isolated%20from%20cultured%20catfish%20by%20antibiotic%20resistance%20and%20RAPD%20analysis.pdf |