Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)
1. The activities of hexokinase and carnitine palmitoyltransferase (CPT) activities were assayed in flight muscle mitochondria of Valanga nigricornis. 2. Kinetic analysis of the enzyme activities yielded the following information: for hexokinase, its Km (glucose) is 150 μM while its Vmax is 2.5 μmol...
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| Format: | Article |
| Language: | English English |
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Pergamon Press
1983
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40470/ http://psasir.upm.edu.my/id/eprint/40470/1/Hexokinase%20and%20carnitine%20palmitoyltransferase%20activities%20in%20flight%20muscles%20of%20grasshopper%20Valanga%20nigricornis%28burm.%29.pdf http://psasir.upm.edu.my/id/eprint/40470/7/40470.pdf |
| _version_ | 1848849434829389824 |
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| author | Lee, C. W. Tan, C. H. |
| author_facet | Lee, C. W. Tan, C. H. |
| author_sort | Lee, C. W. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | 1. The activities of hexokinase and carnitine palmitoyltransferase (CPT) activities were assayed in flight muscle mitochondria of Valanga nigricornis. 2. Kinetic analysis of the enzyme activities yielded the following information: for hexokinase, its Km (glucose) is 150 μM while its Vmax is 2.5 μmol/min/mg protein; the corresponding data for CPT are Km (palmitoylCoA) of 39 μM and Vmax of 22.2 nmol/min/mg mitochondrial protein. 3. Under conditions of equivalent rates of ATP production the hexokinase-CPT activity ratio is 64, suggesting that although V. nigricornis is a “mixed” fuel utilizer, it appears that carbohydrate oxidation constitutes the predominant energy source. |
| first_indexed | 2025-11-15T09:50:20Z |
| format | Article |
| id | upm-40470 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:50:20Z |
| publishDate | 1983 |
| publisher | Pergamon Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-404702024-10-15T06:36:05Z http://psasir.upm.edu.my/id/eprint/40470/ Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.) Lee, C. W. Tan, C. H. 1. The activities of hexokinase and carnitine palmitoyltransferase (CPT) activities were assayed in flight muscle mitochondria of Valanga nigricornis. 2. Kinetic analysis of the enzyme activities yielded the following information: for hexokinase, its Km (glucose) is 150 μM while its Vmax is 2.5 μmol/min/mg protein; the corresponding data for CPT are Km (palmitoylCoA) of 39 μM and Vmax of 22.2 nmol/min/mg mitochondrial protein. 3. Under conditions of equivalent rates of ATP production the hexokinase-CPT activity ratio is 64, suggesting that although V. nigricornis is a “mixed” fuel utilizer, it appears that carbohydrate oxidation constitutes the predominant energy source. Pergamon Press 1983 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/40470/1/Hexokinase%20and%20carnitine%20palmitoyltransferase%20activities%20in%20flight%20muscles%20of%20grasshopper%20Valanga%20nigricornis%28burm.%29.pdf text en http://psasir.upm.edu.my/id/eprint/40470/7/40470.pdf Lee, C. W. and Tan, C. H. (1983) Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 74 (4). pp. 791-792. ISSN 0305-0491 http://www.sciencedirect.com/science/article/pii/0305049183901463 10.1016/0305-0491(83)90146-3 |
| spellingShingle | Lee, C. W. Tan, C. H. Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.) |
| title | Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.) |
| title_full | Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.) |
| title_fullStr | Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.) |
| title_full_unstemmed | Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.) |
| title_short | Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.) |
| title_sort | hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper valanga nigricornis(burm.) |
| url | http://psasir.upm.edu.my/id/eprint/40470/ http://psasir.upm.edu.my/id/eprint/40470/ http://psasir.upm.edu.my/id/eprint/40470/ http://psasir.upm.edu.my/id/eprint/40470/1/Hexokinase%20and%20carnitine%20palmitoyltransferase%20activities%20in%20flight%20muscles%20of%20grasshopper%20Valanga%20nigricornis%28burm.%29.pdf http://psasir.upm.edu.my/id/eprint/40470/7/40470.pdf |