Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates
The increasing prevalence of penicillin-resistant Streptococuus pneumoniae urges for fast and accurate susceptibility testing methods. This study evaluated the comparability of three commonly used techniques; disk diffusion, E-test and agar dilution, to detect penicillin susceptibility in clinical i...
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| Format: | Article |
| Language: | English |
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College of Pathologists, Academy of Medicine Malaysia
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40463/ http://psasir.upm.edu.my/id/eprint/40463/1/Comparison%20of%20susceptibility%20test%20methods%20to%20detect%20penicillin%20susceptibility%20in%20Streptococcus%20pneumoniae%20isolates.pdf |
| _version_ | 1848849432659886080 |
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| author | Mohd Desa, Mohd Nasir Navaratnam, Parasakthi |
| author_facet | Mohd Desa, Mohd Nasir Navaratnam, Parasakthi |
| author_sort | Mohd Desa, Mohd Nasir |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The increasing prevalence of penicillin-resistant Streptococuus pneumoniae urges for fast and accurate susceptibility testing methods. This study evaluated the comparability of three commonly used techniques; disk diffusion, E-test and agar dilution, to detect penicillin susceptibility in clinical isolates of S. pneumoniae. Fifty pneumococcal isolates, obtained from patients at the University of Malaya Medical Centre, were selected to include both penicillin-susceptible strains and those that had decreased susceptibility (resistant and intermediate) to penicillin. The minimum inhibitory concentration (MIC) values of penicillin to serve as the reference was determined by the agar dilution method in which, based on the MIC breakpoints recommended by the National Committee for Clinical Laboratory Standards (NCCLS), 27 strains had decreased susceptibility to penicillin with 17 strains resistant and 10 intermediate. Comparing to the agar dilution method, oxacillin disk diffusion test detected all strains with decreased penicillin susceptibility as such while E-test showed a close agreement of susceptibility (92%) of the isolates to penicillin. This confirmed that oxacillin is a good screening test for S. pneumoniae isolates with decreased susceptibility to penicillin while E-test is very reliable for rapid and accurate detection of penicillin susceptibility. |
| first_indexed | 2025-11-15T09:50:18Z |
| format | Article |
| id | upm-40463 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:50:18Z |
| publishDate | 2004 |
| publisher | College of Pathologists, Academy of Medicine Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-404632015-09-18T04:46:20Z http://psasir.upm.edu.my/id/eprint/40463/ Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates Mohd Desa, Mohd Nasir Navaratnam, Parasakthi The increasing prevalence of penicillin-resistant Streptococuus pneumoniae urges for fast and accurate susceptibility testing methods. This study evaluated the comparability of three commonly used techniques; disk diffusion, E-test and agar dilution, to detect penicillin susceptibility in clinical isolates of S. pneumoniae. Fifty pneumococcal isolates, obtained from patients at the University of Malaya Medical Centre, were selected to include both penicillin-susceptible strains and those that had decreased susceptibility (resistant and intermediate) to penicillin. The minimum inhibitory concentration (MIC) values of penicillin to serve as the reference was determined by the agar dilution method in which, based on the MIC breakpoints recommended by the National Committee for Clinical Laboratory Standards (NCCLS), 27 strains had decreased susceptibility to penicillin with 17 strains resistant and 10 intermediate. Comparing to the agar dilution method, oxacillin disk diffusion test detected all strains with decreased penicillin susceptibility as such while E-test showed a close agreement of susceptibility (92%) of the isolates to penicillin. This confirmed that oxacillin is a good screening test for S. pneumoniae isolates with decreased susceptibility to penicillin while E-test is very reliable for rapid and accurate detection of penicillin susceptibility. College of Pathologists, Academy of Medicine Malaysia 2004-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40463/1/Comparison%20of%20susceptibility%20test%20methods%20to%20detect%20penicillin%20susceptibility%20in%20Streptococcus%20pneumoniae%20isolates.pdf Mohd Desa, Mohd Nasir and Navaratnam, Parasakthi (2004) Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates. Malaysian Journal of Pathology, 26 (1). pp. 29-33. ISSN 0126-8635 http://www.mjpath.org.my/past_issue/MJP2004.1/index.html |
| spellingShingle | Mohd Desa, Mohd Nasir Navaratnam, Parasakthi Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates |
| title | Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates |
| title_full | Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates |
| title_fullStr | Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates |
| title_full_unstemmed | Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates |
| title_short | Comparison of susceptibility test methods to detect penicillin susceptibility in Streptococcus pneumoniae isolates |
| title_sort | comparison of susceptibility test methods to detect penicillin susceptibility in streptococcus pneumoniae isolates |
| url | http://psasir.upm.edu.my/id/eprint/40463/ http://psasir.upm.edu.my/id/eprint/40463/ http://psasir.upm.edu.my/id/eprint/40463/1/Comparison%20of%20susceptibility%20test%20methods%20to%20detect%20penicillin%20susceptibility%20in%20Streptococcus%20pneumoniae%20isolates.pdf |