Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71
This study describes expression of HBs Ag in methylotrophic yeast, Pichia Pastoris under alcohol oxidase promoter. A single copy number of HBs Ag gene was transformed into pichia strain of KM 71, a Mutˢ type, by using pA0815 pichia expression vector. The recombinant was cultivated in a shake flask e...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40425/ http://psasir.upm.edu.my/id/eprint/40425/1/3.%20Effect%20of%20glycerol%20feed%20in%20methanol%20induction%20phase%20for%20hepatitis%20B%20surface%20antigen%20expression%20in%20Pichia%20pastoris%20strain%20KM71.pdf |
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| author | Morvarid, Akhavan Rezaei Allaudin, Zeenathul Nazariah Tam, Yew Joon Hassan, Zuridah Mohd Lila, Mohd Azmi Othman, Azizon |
| author_facet | Morvarid, Akhavan Rezaei Allaudin, Zeenathul Nazariah Tam, Yew Joon Hassan, Zuridah Mohd Lila, Mohd Azmi Othman, Azizon |
| author_sort | Morvarid, Akhavan Rezaei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study describes expression of HBs Ag in methylotrophic yeast, Pichia Pastoris under alcohol oxidase promoter. A single copy number of HBs Ag gene was transformed into pichia strain of KM 71, a Mutˢ type, by using pA0815 pichia expression vector. The recombinant was cultivated in a shake flask either using methanol or a mixed feed of glycerol -methanol for induction. The HBs Ag gene integrity was justified using direct PCR method. The expressed products in the soluble cell extracts were analyzed by Western blot, SDS page, Bradford assay and ELISA tests. The recombinant HBs Ag was expressed successfully in Pichia pastoris strain KM71 at a high level of HBs Ag protein expression. Thus, an addition of glycerol in the ratio of glycerol per methanol 1/1 (g g-1) consistently produced 2-fold increment in both biomass accumulation and HBs Ag productivity. |
| first_indexed | 2025-11-15T09:50:08Z |
| format | Article |
| id | upm-40425 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:50:08Z |
| publishDate | 2012 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-404252019-10-30T06:50:59Z http://psasir.upm.edu.my/id/eprint/40425/ Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71 Morvarid, Akhavan Rezaei Allaudin, Zeenathul Nazariah Tam, Yew Joon Hassan, Zuridah Mohd Lila, Mohd Azmi Othman, Azizon This study describes expression of HBs Ag in methylotrophic yeast, Pichia Pastoris under alcohol oxidase promoter. A single copy number of HBs Ag gene was transformed into pichia strain of KM 71, a Mutˢ type, by using pA0815 pichia expression vector. The recombinant was cultivated in a shake flask either using methanol or a mixed feed of glycerol -methanol for induction. The HBs Ag gene integrity was justified using direct PCR method. The expressed products in the soluble cell extracts were analyzed by Western blot, SDS page, Bradford assay and ELISA tests. The recombinant HBs Ag was expressed successfully in Pichia pastoris strain KM71 at a high level of HBs Ag protein expression. Thus, an addition of glycerol in the ratio of glycerol per methanol 1/1 (g g-1) consistently produced 2-fold increment in both biomass accumulation and HBs Ag productivity. Universiti Putra Malaysia Press 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40425/1/3.%20Effect%20of%20glycerol%20feed%20in%20methanol%20induction%20phase%20for%20hepatitis%20B%20surface%20antigen%20expression%20in%20Pichia%20pastoris%20strain%20KM71.pdf Morvarid, Akhavan Rezaei and Allaudin, Zeenathul Nazariah and Tam, Yew Joon and Hassan, Zuridah and Mohd Lila, Mohd Azmi and Othman, Azizon (2012) Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71. Pertanika Journal of Science & Technology, 20 (1). pp. 31-42. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JST%20Vol.%2020%20%281%29%20Jan.%202012/08%20Pg%2031-42.pdf |
| spellingShingle | Morvarid, Akhavan Rezaei Allaudin, Zeenathul Nazariah Tam, Yew Joon Hassan, Zuridah Mohd Lila, Mohd Azmi Othman, Azizon Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71 |
| title | Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71 |
| title_full | Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71 |
| title_fullStr | Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71 |
| title_full_unstemmed | Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71 |
| title_short | Effect of glycerol feed in methanol induction phase for hepatitis B surface antigen expression in Pichia pastoris strain KM71 |
| title_sort | effect of glycerol feed in methanol induction phase for hepatitis b surface antigen expression in pichia pastoris strain km71 |
| url | http://psasir.upm.edu.my/id/eprint/40425/ http://psasir.upm.edu.my/id/eprint/40425/ http://psasir.upm.edu.my/id/eprint/40425/1/3.%20Effect%20of%20glycerol%20feed%20in%20methanol%20induction%20phase%20for%20hepatitis%20B%20surface%20antigen%20expression%20in%20Pichia%20pastoris%20strain%20KM71.pdf |