Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification
Lipase from Candida rugosa was immobilized onto natural kaolin by physical adsorption method. About 77% of protein content was immobilized onto the support. The activities of the immobilized lipase were determined by the esterification activities using oleic acid and 1-butanol as substrates and hexa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40335/ http://psasir.upm.edu.my/id/eprint/40335/1/Application%20of%20natural%20kaolin%20as%20support%20for%20the%20immobilization%20of%20lipase%20from%20Candida%20rugosa%20as%20biocatalsyt%20for%20effective%20esterification.pdf |
| _version_ | 1848849396522811392 |
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| author | Abdul Rahman, Mohd Basyaruddin Md Tajudin, Safarini Hussein, Mohd Zobir Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_facet | Abdul Rahman, Mohd Basyaruddin Md Tajudin, Safarini Hussein, Mohd Zobir Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_sort | Abdul Rahman, Mohd Basyaruddin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lipase from Candida rugosa was immobilized onto natural kaolin by physical adsorption method. About 77% of protein content was immobilized onto the support. The activities of the immobilized lipase were determined by the esterification activities using oleic acid and 1-butanol as substrates and hexane as reaction medium. The effects of reaction temperature, thermostability, stability in organic solvent, leaching and storage studies of immobilized lipase were investigated. Kaolin-immobilized lipase exhibited activities higher by fourfolds than the native lipase after thermal stability test at 70 °C. Immobilized lipase was found to be stable in hexane at room temperature up to 12 days and also showed higher stability than native lipase in the storage study. Leaching studies showed that the immobilized lipase remained full activity even after being washed by 20 ml of solvent. The experimental results showed that physical adsorption is suitable for the attachment of enzyme onto kaolin. |
| first_indexed | 2025-11-15T09:49:44Z |
| format | Article |
| id | upm-40335 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:49:44Z |
| publishDate | 2005 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-403352016-09-26T08:13:15Z http://psasir.upm.edu.my/id/eprint/40335/ Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification Abdul Rahman, Mohd Basyaruddin Md Tajudin, Safarini Hussein, Mohd Zobir Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Lipase from Candida rugosa was immobilized onto natural kaolin by physical adsorption method. About 77% of protein content was immobilized onto the support. The activities of the immobilized lipase were determined by the esterification activities using oleic acid and 1-butanol as substrates and hexane as reaction medium. The effects of reaction temperature, thermostability, stability in organic solvent, leaching and storage studies of immobilized lipase were investigated. Kaolin-immobilized lipase exhibited activities higher by fourfolds than the native lipase after thermal stability test at 70 °C. Immobilized lipase was found to be stable in hexane at room temperature up to 12 days and also showed higher stability than native lipase in the storage study. Leaching studies showed that the immobilized lipase remained full activity even after being washed by 20 ml of solvent. The experimental results showed that physical adsorption is suitable for the attachment of enzyme onto kaolin. Elsevier 2005-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40335/1/Application%20of%20natural%20kaolin%20as%20support%20for%20the%20immobilization%20of%20lipase%20from%20Candida%20rugosa%20as%20biocatalsyt%20for%20effective%20esterification.pdf Abdul Rahman, Mohd Basyaruddin and Md Tajudin, Safarini and Hussein, Mohd Zobir and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2005) Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification. Applied Clay Science, 29 (2). pp. 111-116. ISSN 0169-1317; ESSN: 1872-9053 http://www.sciencedirect.com/science/article/pii/S0169131704001887 10.1016/j.clay.2004.12.001 |
| spellingShingle | Abdul Rahman, Mohd Basyaruddin Md Tajudin, Safarini Hussein, Mohd Zobir Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification |
| title | Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification |
| title_full | Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification |
| title_fullStr | Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification |
| title_full_unstemmed | Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification |
| title_short | Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalsyt for effective esterification |
| title_sort | application of natural kaolin as support for the immobilization of lipase from candida rugosa as biocatalsyt for effective esterification |
| url | http://psasir.upm.edu.my/id/eprint/40335/ http://psasir.upm.edu.my/id/eprint/40335/ http://psasir.upm.edu.my/id/eprint/40335/ http://psasir.upm.edu.my/id/eprint/40335/1/Application%20of%20natural%20kaolin%20as%20support%20for%20the%20immobilization%20of%20lipase%20from%20Candida%20rugosa%20as%20biocatalsyt%20for%20effective%20esterification.pdf |