Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells
Styryl-lactones such as goniothalamin represent a new class of compounds with potential anti-cancer properties. In this study, we investigated the mechanisms of goniothalamin (GTN), a plant styryl-lactone induced apoptosis in human promyelocytic leukemia HL-60 cells. This plant extract resulted in a...
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Elsevier
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40315/ http://psasir.upm.edu.my/id/eprint/40315/1/Loss%20of%20mitochondrial%20transmembrane%20potential%20and%20caspase-9%20activation%20during%20apoptosis%20induced%20by%20the%20novel%20styryl-lactone%20goniothalamin%20in%20HL-60%20leukemia%20cells.pdf http://psasir.upm.edu.my/id/eprint/40315/7/1-s2.0-S0887233303000511-main.pdf |
| _version_ | 1848849390974795776 |
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| author | Inayat Hussain, Salmaan Hussain Osman, Md. Anuar Din, Laily Ali, Abdul Manaf Ross, David |
| author_facet | Inayat Hussain, Salmaan Hussain Osman, Md. Anuar Din, Laily Ali, Abdul Manaf Ross, David |
| author_sort | Inayat Hussain, Salmaan Hussain |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Styryl-lactones such as goniothalamin represent a new class of compounds with potential anti-cancer properties. In this study, we investigated the mechanisms of goniothalamin (GTN), a plant styryl-lactone induced apoptosis in human promyelocytic leukemia HL-60 cells. This plant extract resulted in apoptosis in HL-60 cells as assessed by the externalisation of phosphatidylserine. Using the mitochondrial membrane dye (DIOC6) in conjunction with flow cytometry, we found that GTN treated HL-60 cells demonstrated a loss of mitochondrial transmembrane potential (Δψm). Further immunoblotting on these cells showed activation of initiator caspase-9 and the executioner caspases-3 and -7. Pretreatment with the pharmacological caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) abrogated apoptosis as assessed by all of the apoptotic features in this study. In summary, our results demonstrate that goniothalamin-induced apoptosis occurs via the mitochondrial pathway in a caspase dependent manner. |
| first_indexed | 2025-11-15T09:49:39Z |
| format | Article |
| id | upm-40315 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:49:39Z |
| publishDate | 2003 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-403152024-07-24T06:28:46Z http://psasir.upm.edu.my/id/eprint/40315/ Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells Inayat Hussain, Salmaan Hussain Osman, Md. Anuar Din, Laily Ali, Abdul Manaf Ross, David Styryl-lactones such as goniothalamin represent a new class of compounds with potential anti-cancer properties. In this study, we investigated the mechanisms of goniothalamin (GTN), a plant styryl-lactone induced apoptosis in human promyelocytic leukemia HL-60 cells. This plant extract resulted in apoptosis in HL-60 cells as assessed by the externalisation of phosphatidylserine. Using the mitochondrial membrane dye (DIOC6) in conjunction with flow cytometry, we found that GTN treated HL-60 cells demonstrated a loss of mitochondrial transmembrane potential (Δψm). Further immunoblotting on these cells showed activation of initiator caspase-9 and the executioner caspases-3 and -7. Pretreatment with the pharmacological caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) abrogated apoptosis as assessed by all of the apoptotic features in this study. In summary, our results demonstrate that goniothalamin-induced apoptosis occurs via the mitochondrial pathway in a caspase dependent manner. Elsevier 2003-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/40315/1/Loss%20of%20mitochondrial%20transmembrane%20potential%20and%20caspase-9%20activation%20during%20apoptosis%20induced%20by%20the%20novel%20styryl-lactone%20goniothalamin%20in%20HL-60%20leukemia%20cells.pdf text en http://psasir.upm.edu.my/id/eprint/40315/7/1-s2.0-S0887233303000511-main.pdf Inayat Hussain, Salmaan Hussain and Osman, Md. Anuar and Din, Laily and Ali, Abdul Manaf and Ross, David (2003) Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells. Toxicology in Vitro, 17 (4). pp. 433-439. ISSN 0887-2333; ESSN: 1879-3177 http://www.sciencedirect.com/science/article/pii/S0887233303000511 10.1016/S0887-2333(03)00051-1 |
| spellingShingle | Inayat Hussain, Salmaan Hussain Osman, Md. Anuar Din, Laily Ali, Abdul Manaf Ross, David Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells |
| title | Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells |
| title_full | Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells |
| title_fullStr | Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells |
| title_full_unstemmed | Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells |
| title_short | Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells |
| title_sort | loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in hl-60 leukemia cells |
| url | http://psasir.upm.edu.my/id/eprint/40315/ http://psasir.upm.edu.my/id/eprint/40315/ http://psasir.upm.edu.my/id/eprint/40315/ http://psasir.upm.edu.my/id/eprint/40315/1/Loss%20of%20mitochondrial%20transmembrane%20potential%20and%20caspase-9%20activation%20during%20apoptosis%20induced%20by%20the%20novel%20styryl-lactone%20goniothalamin%20in%20HL-60%20leukemia%20cells.pdf http://psasir.upm.edu.my/id/eprint/40315/7/1-s2.0-S0887233303000511-main.pdf |