Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent

A hydrogen producer was successfully isolated from anaerobic digested palm oil mill effluent (POME) sludge. The strain, designated as Clostridium butyricum EB6, efficiently produced hydrogen concurrently with cell growth. A controlled study was done on a synthetic medium at an initial pH value of 6....

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Main Authors: Chong, Mei Ling, Abdul Rahim, Raha, Shirai, Yoshihito, Hassan, Mohd Ali
Format: Article
Language:English
Published: Elsevier 2009
Online Access:http://psasir.upm.edu.my/id/eprint/40231/
http://psasir.upm.edu.my/id/eprint/40231/1/Biohydrogen%20production%20by%20Clostridium%20butyricum%20EB6%20from%20palm%20oil%20mill%20effluent.pdf
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author Chong, Mei Ling
Abdul Rahim, Raha
Shirai, Yoshihito
Hassan, Mohd Ali
author_facet Chong, Mei Ling
Abdul Rahim, Raha
Shirai, Yoshihito
Hassan, Mohd Ali
author_sort Chong, Mei Ling
building UPM Institutional Repository
collection Online Access
description A hydrogen producer was successfully isolated from anaerobic digested palm oil mill effluent (POME) sludge. The strain, designated as Clostridium butyricum EB6, efficiently produced hydrogen concurrently with cell growth. A controlled study was done on a synthetic medium at an initial pH value of 6.0 with 10 g/L glucose with the maximum hydrogen production at 948 mL H2/L-medium and the volumetric hydrogen production rate at 172 mL H2/L-medium/h. The supplementation of yeast extract was shown to have a significant effect with a maximum hydrogen production of 992 mL H2/L-medium at 4 g/L of yeast extract added. The effect of pH on hydrogen production from POME was investigated. Experimental results showed that the optimum hydrogen production ability occurred at pH 5.5. The maximum hydrogen production and maximum volumetric hydrogen production rate were at 3195 mL H2/L-medium and 1034 mL H2/L-medium/h, respectively. The hydrogen content in the biogas produced was in the range of 60–70%.
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spelling upm-402312015-09-14T06:37:14Z http://psasir.upm.edu.my/id/eprint/40231/ Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent Chong, Mei Ling Abdul Rahim, Raha Shirai, Yoshihito Hassan, Mohd Ali A hydrogen producer was successfully isolated from anaerobic digested palm oil mill effluent (POME) sludge. The strain, designated as Clostridium butyricum EB6, efficiently produced hydrogen concurrently with cell growth. A controlled study was done on a synthetic medium at an initial pH value of 6.0 with 10 g/L glucose with the maximum hydrogen production at 948 mL H2/L-medium and the volumetric hydrogen production rate at 172 mL H2/L-medium/h. The supplementation of yeast extract was shown to have a significant effect with a maximum hydrogen production of 992 mL H2/L-medium at 4 g/L of yeast extract added. The effect of pH on hydrogen production from POME was investigated. Experimental results showed that the optimum hydrogen production ability occurred at pH 5.5. The maximum hydrogen production and maximum volumetric hydrogen production rate were at 3195 mL H2/L-medium and 1034 mL H2/L-medium/h, respectively. The hydrogen content in the biogas produced was in the range of 60–70%. Elsevier 2009-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40231/1/Biohydrogen%20production%20by%20Clostridium%20butyricum%20EB6%20from%20palm%20oil%20mill%20effluent.pdf Chong, Mei Ling and Abdul Rahim, Raha and Shirai, Yoshihito and Hassan, Mohd Ali (2009) Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent. International Journal of Hydrogen Energy, 34 (2). pp. 764-771. ISSN 0360-3199; ESSN: 1879-3487 http://www.sciencedirect.com/science/article/pii/S0360319908014444 10.1016/j.ijhydene.2008.10.095
spellingShingle Chong, Mei Ling
Abdul Rahim, Raha
Shirai, Yoshihito
Hassan, Mohd Ali
Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent
title Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent
title_full Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent
title_fullStr Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent
title_full_unstemmed Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent
title_short Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent
title_sort biohydrogen production by clostridium butyricum eb6 from palm oil mill effluent
url http://psasir.upm.edu.my/id/eprint/40231/
http://psasir.upm.edu.my/id/eprint/40231/
http://psasir.upm.edu.my/id/eprint/40231/
http://psasir.upm.edu.my/id/eprint/40231/1/Biohydrogen%20production%20by%20Clostridium%20butyricum%20EB6%20from%20palm%20oil%20mill%20effluent.pdf