Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA

Enterotoxigenic Bacillus cereus was detected in cooked foods (17), rice noodles (3), wet wheat noodles (2), dry wheat noodles (10), spices (8), grains (4), legumes (11) and legume products (3). One hundred ninety-four (42.3%), 70 (15.3%) and 23 (5.2%) of the 459 presumptive B. cereus colonies isolat...

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Main Authors: Rusul, Gulam, Yaacob, Nur Hayati
Format: Article
Language:English
English
Published: Elsevier 1995
Online Access:http://psasir.upm.edu.my/id/eprint/40011/
http://psasir.upm.edu.my/id/eprint/40011/1/Prevalence%20of%20Bacillus%20cereus%20in%20selected%20foods%20and%20detection%20of%20enterotoxin%20using%20TECRA-VIA%20and%20BCET-RPLA.pdf
http://psasir.upm.edu.my/id/eprint/40011/7/1-s2.0-016816059400086L-main.pdf
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author Rusul, Gulam
Yaacob, Nur Hayati
author_facet Rusul, Gulam
Yaacob, Nur Hayati
author_sort Rusul, Gulam
building UPM Institutional Repository
collection Online Access
description Enterotoxigenic Bacillus cereus was detected in cooked foods (17), rice noodles (3), wet wheat noodles (2), dry wheat noodles (10), spices (8), grains (4), legumes (11) and legume products (3). One hundred ninety-four (42.3%), 70 (15.3%) and 23 (5.2%) of the 459 presumptive B. cereus colonies isolated from PEMBA agar were identified as B. cereus, Bacillus thuringiensis and B. mycoides, respectively. B. cereus isolates were examined for growth temperature, pH profile and enterotoxin production using both TECRA-VIA and BCET-RPLA kits. One hundred seventy-eight (91.8%) and 164 (84.%) of the strains were enterotoxigenic as determined using TECRA-VIA and BCET-RPLA, respectively. Eighty-two (50%) of the enterotoxigenic strains were capable of growing at 5 °C, and 142 (86.6%) grew at 7 °C within 7 days of incubation. The enterotoxigenic strains did not grow at pH 4.0 but 69 (42.0%) of the strains were able to grow at pH 4.5 within 7 days at 37 °C. The isolates were resistant to ampicillin (98.8%), cloxallin (100%) and tetracycline (61.0%), and susceptible to chloroamphenicol (87%), erythromycin (77.4%), gentamycin (100%) and streptomycin (98.7%).
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spelling upm-400112024-07-24T06:23:35Z http://psasir.upm.edu.my/id/eprint/40011/ Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA Rusul, Gulam Yaacob, Nur Hayati Enterotoxigenic Bacillus cereus was detected in cooked foods (17), rice noodles (3), wet wheat noodles (2), dry wheat noodles (10), spices (8), grains (4), legumes (11) and legume products (3). One hundred ninety-four (42.3%), 70 (15.3%) and 23 (5.2%) of the 459 presumptive B. cereus colonies isolated from PEMBA agar were identified as B. cereus, Bacillus thuringiensis and B. mycoides, respectively. B. cereus isolates were examined for growth temperature, pH profile and enterotoxin production using both TECRA-VIA and BCET-RPLA kits. One hundred seventy-eight (91.8%) and 164 (84.%) of the strains were enterotoxigenic as determined using TECRA-VIA and BCET-RPLA, respectively. Eighty-two (50%) of the enterotoxigenic strains were capable of growing at 5 °C, and 142 (86.6%) grew at 7 °C within 7 days of incubation. The enterotoxigenic strains did not grow at pH 4.0 but 69 (42.0%) of the strains were able to grow at pH 4.5 within 7 days at 37 °C. The isolates were resistant to ampicillin (98.8%), cloxallin (100%) and tetracycline (61.0%), and susceptible to chloroamphenicol (87%), erythromycin (77.4%), gentamycin (100%) and streptomycin (98.7%). Elsevier 1995-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/40011/1/Prevalence%20of%20Bacillus%20cereus%20in%20selected%20foods%20and%20detection%20of%20enterotoxin%20using%20TECRA-VIA%20and%20BCET-RPLA.pdf text en http://psasir.upm.edu.my/id/eprint/40011/7/1-s2.0-016816059400086L-main.pdf Rusul, Gulam and Yaacob, Nur Hayati (1995) Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA. International Journal of Food Microbiology, 25 (2). pp. 131-139. ISSN 0168-1605; ESSN: 1879-3460 10.1016/0168-1605(94)00086-L
spellingShingle Rusul, Gulam
Yaacob, Nur Hayati
Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA
title Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA
title_full Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA
title_fullStr Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA
title_full_unstemmed Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA
title_short Prevalence of Bacillus cereus in selected foods and detection of enterotoxin using TECRA-VIA and BCET-RPLA
title_sort prevalence of bacillus cereus in selected foods and detection of enterotoxin using tecra-via and bcet-rpla
url http://psasir.upm.edu.my/id/eprint/40011/
http://psasir.upm.edu.my/id/eprint/40011/
http://psasir.upm.edu.my/id/eprint/40011/1/Prevalence%20of%20Bacillus%20cereus%20in%20selected%20foods%20and%20detection%20of%20enterotoxin%20using%20TECRA-VIA%20and%20BCET-RPLA.pdf
http://psasir.upm.edu.my/id/eprint/40011/7/1-s2.0-016816059400086L-main.pdf