Determination of fetal sex in cattle by cytologic examination of amniotic fluid

Amniotic fluid smears of bovine fetuses (69 males and 65 females) at various stages of gestation were stained by the Papanicolaou method. Percentage counts of eosinophilic, orangeophilic and cyanophilic cells were determined by examining 500 cells per sample. The cyanophilic cell count was significa...

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Bibliographic Details
Main Authors: Bongso, Tuan Ariffeen, Wettimuny, S. G. de S, Edirisinghe, R., Kumaratileke, L.
Format: Article
Language:English
Published: American Veterinary Medical Association 1978
Online Access:http://psasir.upm.edu.my/id/eprint/39544/
http://psasir.upm.edu.my/id/eprint/39544/1/26%20-%20Determination%20of%20fetal%20sex%20in%20cattle%20by%20cytologic%20examination%20of%20amniotic%20fluid.pdf
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Summary:Amniotic fluid smears of bovine fetuses (69 males and 65 females) at various stages of gestation were stained by the Papanicolaou method. Percentage counts of eosinophilic, orangeophilic and cyanophilic cells were determined by examining 500 cells per sample. The cyanophilic cell count was significantly (P < 0.001) higher for the females (37% .+-. 3% to 47% .+-. 3%) than for the males (19% .+-. 2% to 32% .+-. 3%). Significant differences were not recorded with the eosinophilic and the orangeophilic cell counts. The percentage cyanophilic count could therefore be used as a method of prenatal sex determination