Acceptability of crackers (‘Keropok’) with fish protein hydrolysate

Proteins from Oreochromis mossambicus, a freshwater fish, were hydrolysed, using alkalase 0.61, to produce a soluble, spray-dried hydrolysate. The hydrolysis was carried out at 50°C, using a ratio of one part water and one part fish mince, an enzyme:substrate ratio of 1:50 at pH 8.0. Reaction was te...

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Main Authors: Yu, S. Y., Tan, L. K.
Format: Article
Language:English
Published: Institute of Food Science and Technology 1990
Online Access:http://psasir.upm.edu.my/id/eprint/39528/
http://psasir.upm.edu.my/id/eprint/39528/1/Acceptability%20of%20crackers%20%28%27keropok%27%29%20with%20fish%20protein%20hydrolysate.pdf
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author Yu, S. Y.
Tan, L. K.
author_facet Yu, S. Y.
Tan, L. K.
author_sort Yu, S. Y.
building UPM Institutional Repository
collection Online Access
description Proteins from Oreochromis mossambicus, a freshwater fish, were hydrolysed, using alkalase 0.61, to produce a soluble, spray-dried hydrolysate. The hydrolysis was carried out at 50°C, using a ratio of one part water and one part fish mince, an enzyme:substrate ratio of 1:50 at pH 8.0. Reaction was terminated by heating to 90°C for 20min. After neutralization, the soluble fraction obtained after centrifugation was spray dried in a mini spray-drier, at an air inlet temperature of 170°C and a feed rate of 41−1. The spray-dried hydrolysate was incorporated into crackers which are fried before eating. Ten per cent hydrolysate was found to give maximum linear expansion. Sensory evaluation with 20 experienced assessors showed that in terms of appearance, crispiness and colour, crackers with hydrolysate had the highest scores, compared to crackers made with O. mossambicus and Sciaena sp. (Jewfish). There were no significant differences in overall acceptability in all three samples. Crackers with hydrolysate also had the highest nitrogen content.
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spelling upm-395282015-08-10T04:06:44Z http://psasir.upm.edu.my/id/eprint/39528/ Acceptability of crackers (‘Keropok’) with fish protein hydrolysate Yu, S. Y. Tan, L. K. Proteins from Oreochromis mossambicus, a freshwater fish, were hydrolysed, using alkalase 0.61, to produce a soluble, spray-dried hydrolysate. The hydrolysis was carried out at 50°C, using a ratio of one part water and one part fish mince, an enzyme:substrate ratio of 1:50 at pH 8.0. Reaction was terminated by heating to 90°C for 20min. After neutralization, the soluble fraction obtained after centrifugation was spray dried in a mini spray-drier, at an air inlet temperature of 170°C and a feed rate of 41−1. The spray-dried hydrolysate was incorporated into crackers which are fried before eating. Ten per cent hydrolysate was found to give maximum linear expansion. Sensory evaluation with 20 experienced assessors showed that in terms of appearance, crispiness and colour, crackers with hydrolysate had the highest scores, compared to crackers made with O. mossambicus and Sciaena sp. (Jewfish). There were no significant differences in overall acceptability in all three samples. Crackers with hydrolysate also had the highest nitrogen content. Institute of Food Science and Technology 1990-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/39528/1/Acceptability%20of%20crackers%20%28%27keropok%27%29%20with%20fish%20protein%20hydrolysate.pdf Yu, S. Y. and Tan, L. K. (1990) Acceptability of crackers (‘Keropok’) with fish protein hydrolysate. International Journal of Food Science & Technology, 25 (2). pp. 204-208. ISSN 0950-5423; ESSN: 1365-2621
spellingShingle Yu, S. Y.
Tan, L. K.
Acceptability of crackers (‘Keropok’) with fish protein hydrolysate
title Acceptability of crackers (‘Keropok’) with fish protein hydrolysate
title_full Acceptability of crackers (‘Keropok’) with fish protein hydrolysate
title_fullStr Acceptability of crackers (‘Keropok’) with fish protein hydrolysate
title_full_unstemmed Acceptability of crackers (‘Keropok’) with fish protein hydrolysate
title_short Acceptability of crackers (‘Keropok’) with fish protein hydrolysate
title_sort acceptability of crackers (‘keropok’) with fish protein hydrolysate
url http://psasir.upm.edu.my/id/eprint/39528/
http://psasir.upm.edu.my/id/eprint/39528/1/Acceptability%20of%20crackers%20%28%27keropok%27%29%20with%20fish%20protein%20hydrolysate.pdf