A simple technique for the study of fish meiotic chromosomes
A simple technique to study meiotic chromosomes from the testes of fish injected with colchicine is described. The minced testes was treated with a hypotonic solution of 0.075 M potassium chloride followed by fixation with Carnoy's solution. Air dried slides containing three to four drops of ce...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society of Applied Biology
1982
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| Online Access: | http://psasir.upm.edu.my/id/eprint/39525/ http://psasir.upm.edu.my/id/eprint/39525/1/12%20-%20A%20simple%20technique%20for%20the%20study%20of%20fish%20meiotic%20chromosomes.pdf |
| _version_ | 1848849166449508352 |
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| author | Bongso, Tuan Ariffeen Mohammad Mohsin, Abu Khair Siraj, Siti Shapor |
| author_facet | Bongso, Tuan Ariffeen Mohammad Mohsin, Abu Khair Siraj, Siti Shapor |
| author_sort | Bongso, Tuan Ariffeen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A simple technique to study meiotic chromosomes from the testes of fish injected with colchicine is described. The minced testes was treated with a hypotonic solution of 0.075 M potassium chloride followed by fixation with Carnoy's solution. Air dried slides containing three to four drops of cell suspension were stained with 2 per cent aceto-orcein to display the various stages of meiosis. Clarity of chromosomes and adequate numbers of metaphase spreads per sample were obtained. Satisfactory results were also achieved for mitotic chromosomes when the same technique was used on gill preparations |
| first_indexed | 2025-11-15T09:46:04Z |
| format | Article |
| id | upm-39525 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:46:04Z |
| publishDate | 1982 |
| publisher | Malaysian Society of Applied Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-395252015-08-11T01:47:54Z http://psasir.upm.edu.my/id/eprint/39525/ A simple technique for the study of fish meiotic chromosomes Bongso, Tuan Ariffeen Mohammad Mohsin, Abu Khair Siraj, Siti Shapor A simple technique to study meiotic chromosomes from the testes of fish injected with colchicine is described. The minced testes was treated with a hypotonic solution of 0.075 M potassium chloride followed by fixation with Carnoy's solution. Air dried slides containing three to four drops of cell suspension were stained with 2 per cent aceto-orcein to display the various stages of meiosis. Clarity of chromosomes and adequate numbers of metaphase spreads per sample were obtained. Satisfactory results were also achieved for mitotic chromosomes when the same technique was used on gill preparations Malaysian Society of Applied Biology 1982 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/39525/1/12%20-%20A%20simple%20technique%20for%20the%20study%20of%20fish%20meiotic%20chromosomes.pdf Bongso, Tuan Ariffeen and Mohammad Mohsin, Abu Khair and Siraj, Siti Shapor (1982) A simple technique for the study of fish meiotic chromosomes. Malaysian Applied Biology, 11 (1). pp. 47-49. ISSN 0126-8643 |
| spellingShingle | Bongso, Tuan Ariffeen Mohammad Mohsin, Abu Khair Siraj, Siti Shapor A simple technique for the study of fish meiotic chromosomes |
| title | A simple technique for the study of fish meiotic chromosomes
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| title_full | A simple technique for the study of fish meiotic chromosomes
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| title_fullStr | A simple technique for the study of fish meiotic chromosomes
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| title_full_unstemmed | A simple technique for the study of fish meiotic chromosomes
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| title_short | A simple technique for the study of fish meiotic chromosomes
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| title_sort | simple technique for the study of fish meiotic chromosomes |
| url | http://psasir.upm.edu.my/id/eprint/39525/ http://psasir.upm.edu.my/id/eprint/39525/1/12%20-%20A%20simple%20technique%20for%20the%20study%20of%20fish%20meiotic%20chromosomes.pdf |