Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox
A DNA biosensor detection of oligonucleotides via the interactions of porcine DNA with redox active complex based on the electrochemical transduction is described. A ruthenium(II) complex, [Ru(bpy)2(PIP)]2+ , (bpy = 2,2′bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as DNA label has...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
AIP Publishing
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/39379/ http://psasir.upm.edu.my/id/eprint/39379/1/Electrochemical%20DNA%20biosensor%20for%20detection%20of%20porcine%20oligonucleotides%20using%20ruthenium%28II%29%20complex%20as%20intercalator%20label%20redox.pdf |
| _version_ | 1848849128109375488 |
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| author | Abdullah Halid, Nurul Izni Hasbullah, Siti Aishah Ahmad, Haslina Lee, Yook Heng Abd Karim, Nurul Huda Harun, Siti Norain |
| author_facet | Abdullah Halid, Nurul Izni Hasbullah, Siti Aishah Ahmad, Haslina Lee, Yook Heng Abd Karim, Nurul Huda Harun, Siti Norain |
| author_sort | Abdullah Halid, Nurul Izni |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A DNA biosensor detection of oligonucleotides via the interactions of porcine DNA with redox active complex based on the electrochemical transduction is described. A ruthenium(II) complex, [Ru(bpy)2(PIP)]2+ , (bpy = 2,2′bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as DNA label has been synthesized and characterized by 1H NMR and mass spectra. The study was carried out by covalent bonding immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy)2(PIP)]2+ was used as electrochemical redox intercalator label to detect DNA hybridization event. Electrochemical detection was performed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) over the potential range where the ruthenium (II) complex was active. The results indicate that the interaction of [Ru(bpy)2(PIP)]2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. |
| first_indexed | 2025-11-15T09:45:28Z |
| format | Conference or Workshop Item |
| id | upm-39379 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:45:28Z |
| publishDate | 2014 |
| publisher | AIP Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-393792016-07-28T09:08:42Z http://psasir.upm.edu.my/id/eprint/39379/ Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox Abdullah Halid, Nurul Izni Hasbullah, Siti Aishah Ahmad, Haslina Lee, Yook Heng Abd Karim, Nurul Huda Harun, Siti Norain A DNA biosensor detection of oligonucleotides via the interactions of porcine DNA with redox active complex based on the electrochemical transduction is described. A ruthenium(II) complex, [Ru(bpy)2(PIP)]2+ , (bpy = 2,2′bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as DNA label has been synthesized and characterized by 1H NMR and mass spectra. The study was carried out by covalent bonding immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy)2(PIP)]2+ was used as electrochemical redox intercalator label to detect DNA hybridization event. Electrochemical detection was performed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) over the potential range where the ruthenium (II) complex was active. The results indicate that the interaction of [Ru(bpy)2(PIP)]2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. AIP Publishing 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/39379/1/Electrochemical%20DNA%20biosensor%20for%20detection%20of%20porcine%20oligonucleotides%20using%20ruthenium%28II%29%20complex%20as%20intercalator%20label%20redox.pdf Abdullah Halid, Nurul Izni and Hasbullah, Siti Aishah and Ahmad, Haslina and Lee, Yook Heng and Abd Karim, Nurul Huda and Harun, Siti Norain (2014) Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox. In: The 2014 UKM FST Postgraduate Colloquium, 9-11 Apr. 2014, Selangor, Malaysia. (pp. 313-316). 10.1063/1.4895214 |
| spellingShingle | Abdullah Halid, Nurul Izni Hasbullah, Siti Aishah Ahmad, Haslina Lee, Yook Heng Abd Karim, Nurul Huda Harun, Siti Norain Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox |
| title | Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox |
| title_full | Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox |
| title_fullStr | Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox |
| title_full_unstemmed | Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox |
| title_short | Electrochemical DNA biosensor for detection of porcine oligonucleotides using ruthenium(II) complex as intercalator label redox |
| title_sort | electrochemical dna biosensor for detection of porcine oligonucleotides using ruthenium(ii) complex as intercalator label redox |
| url | http://psasir.upm.edu.my/id/eprint/39379/ http://psasir.upm.edu.my/id/eprint/39379/ http://psasir.upm.edu.my/id/eprint/39379/1/Electrochemical%20DNA%20biosensor%20for%20detection%20of%20porcine%20oligonucleotides%20using%20ruthenium%28II%29%20complex%20as%20intercalator%20label%20redox.pdf |