Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor
A study was done to determine the viability improvement of hybridoma cell producing monoclonal antibody. Using the optimized media, the RC1 hybridoma cells were cultured in T-Flask, MiniPerm bioreactor, and stirred tank bioreactor. The study show that the performance of hybridoma cells growth in the...
| Main Authors: | , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2005
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/38791/ http://psasir.upm.edu.my/id/eprint/38791/1/38791.pdf |
| _version_ | 1848848971439538176 |
|---|---|
| author | Mel, Maizirwan Abdul Rahman, Abdul Rafiz Hasim, Yumi Hasyun Ali, Abdul Manaf |
| author_facet | Mel, Maizirwan Abdul Rahman, Abdul Rafiz Hasim, Yumi Hasyun Ali, Abdul Manaf |
| author_sort | Mel, Maizirwan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A study was done to determine the viability improvement of hybridoma cell producing monoclonal antibody. Using the optimized media, the RC1 hybridoma cells were cultured in T-Flask, MiniPerm bioreactor, and stirred tank bioreactor. The study show that the performance of hybridoma cells growth in the three different culture systems has revealed that in stirred tank bioreactor gave the highest cell percentage of viability (> 90% for five days) and the highest specific growth rate. The growth kinetic values for specific growth rate (μ) obtained from T-Flask, MiniPerm and stirred bioreactor were 0.0151, 0.0243 and 0.0289h-1, respectively. While, the values of doubling time (td) obtained from T-Flask, MiniPerm and stirred bioreactor were 46, 29, and 24h, respectively. |
| first_indexed | 2025-11-15T09:42:58Z |
| format | Conference or Workshop Item |
| id | upm-38791 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:42:58Z |
| publishDate | 2005 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-387912015-06-16T02:45:54Z http://psasir.upm.edu.my/id/eprint/38791/ Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor Mel, Maizirwan Abdul Rahman, Abdul Rafiz Hasim, Yumi Hasyun Ali, Abdul Manaf A study was done to determine the viability improvement of hybridoma cell producing monoclonal antibody. Using the optimized media, the RC1 hybridoma cells were cultured in T-Flask, MiniPerm bioreactor, and stirred tank bioreactor. The study show that the performance of hybridoma cells growth in the three different culture systems has revealed that in stirred tank bioreactor gave the highest cell percentage of viability (> 90% for five days) and the highest specific growth rate. The growth kinetic values for specific growth rate (μ) obtained from T-Flask, MiniPerm and stirred bioreactor were 0.0151, 0.0243 and 0.0289h-1, respectively. While, the values of doubling time (td) obtained from T-Flask, MiniPerm and stirred bioreactor were 46, 29, and 24h, respectively. 2005 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/38791/1/38791.pdf Mel, Maizirwan and Abdul Rahman, Abdul Rafiz and Hasim, Yumi Hasyun and Ali, Abdul Manaf (2005) Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor. In: International Advanced Technology Congress: Conference on Computer Integrated Systems, 6 - 8 Dec. 2005, Putrajaya, Malaysia. . |
| spellingShingle | Mel, Maizirwan Abdul Rahman, Abdul Rafiz Hasim, Yumi Hasyun Ali, Abdul Manaf Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor |
| title | Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor |
| title_full | Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor |
| title_fullStr | Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor |
| title_full_unstemmed | Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor |
| title_short | Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor |
| title_sort | monoclonal antibody production: viability improvement of rc1 hybridoma cell in different types of bioreactor |
| url | http://psasir.upm.edu.my/id/eprint/38791/ http://psasir.upm.edu.my/id/eprint/38791/1/38791.pdf |