Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells
Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin...
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| Format: | Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38409/ http://psasir.upm.edu.my/id/eprint/38409/1/38409.pdf |
| _version_ | 1848848871464108032 |
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| author | Aliyu, Isah Abubakar Ling, King Hwa Md Hashim, Nur Fariesha Lam, Jia Yong Chee, Hui Yee |
| author_facet | Aliyu, Isah Abubakar Ling, King Hwa Md Hashim, Nur Fariesha Lam, Jia Yong Chee, Hui Yee |
| author_sort | Aliyu, Isah Abubakar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediated inhibition of infection and siRNA-mediated knockdown of annexin II expression significantly reduced DENV2 infection and production levels. Collectively, we demonstrated that annexin II is one of the host factor involved in DENV2 binding on Vero cells. |
| first_indexed | 2025-11-15T09:41:23Z |
| format | Article |
| id | upm-38409 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:41:23Z |
| publishDate | 2019 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-384092020-05-04T16:47:02Z http://psasir.upm.edu.my/id/eprint/38409/ Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells Aliyu, Isah Abubakar Ling, King Hwa Md Hashim, Nur Fariesha Lam, Jia Yong Chee, Hui Yee Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediated inhibition of infection and siRNA-mediated knockdown of annexin II expression significantly reduced DENV2 infection and production levels. Collectively, we demonstrated that annexin II is one of the host factor involved in DENV2 binding on Vero cells. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38409/1/38409.pdf Aliyu, Isah Abubakar and Ling, King Hwa and Md Hashim, Nur Fariesha and Lam, Jia Yong and Chee, Hui Yee (2019) Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells. Viruses, 11 (4). art. no. 335. pp. 1-12. ISSN 1999-4915 https://www.mdpi.com/1999-4915/11/4/335 10.3390/v11040335 |
| spellingShingle | Aliyu, Isah Abubakar Ling, King Hwa Md Hashim, Nur Fariesha Lam, Jia Yong Chee, Hui Yee Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells |
| title | Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells |
| title_full | Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells |
| title_fullStr | Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells |
| title_full_unstemmed | Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells |
| title_short | Annexin II as a dengue virus serotype 2 interacting protein mediating virus interaction on Vero cells |
| title_sort | annexin ii as a dengue virus serotype 2 interacting protein mediating virus interaction on vero cells |
| url | http://psasir.upm.edu.my/id/eprint/38409/ http://psasir.upm.edu.my/id/eprint/38409/ http://psasir.upm.edu.my/id/eprint/38409/ http://psasir.upm.edu.my/id/eprint/38409/1/38409.pdf |