Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt
There has been an explosion of probiotic incorporated based product. However, many reports indicated that most of the probiotics have failed to survive in high quantity, which has limited their effectiveness in most functional foods. Thus, to overcome this problem, microencapsulation is considered t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38401/ http://psasir.upm.edu.my/id/eprint/38401/1/38401.pdf |
| _version_ | 1848848869282021376 |
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| author | Fazilah, Nurul Farhana Hamidon, Nurmelissa Hanani Ariff, Arbakariya Khayat, Mohd Ezuan Wasoh @ Mohamad Isa, Helmi Halim, Murni |
| author_facet | Fazilah, Nurul Farhana Hamidon, Nurmelissa Hanani Ariff, Arbakariya Khayat, Mohd Ezuan Wasoh @ Mohamad Isa, Helmi Halim, Murni |
| author_sort | Fazilah, Nurul Farhana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | There has been an explosion of probiotic incorporated based product. However, many reports indicated that most of the probiotics have failed to survive in high quantity, which has limited their effectiveness in most functional foods. Thus, to overcome this problem, microencapsulation is considered to be a promising process. In this study, Lactococcus lactis Gh1 was encapsulated via spray-drying with gum Arabic together with Synsepalum dulcificum or commonly known as miracle fruit. It was observed that after spray-drying, high viability (~109 CFU/mL) powders containing L. lactis in combination with S. dulcificum were developed, which was then formulated into yogurt. The tolerance of encapsulated bacterial cells in simulated gastric juice at pH 1.5 was tested in an in-vitro model and the result showed that after 2 h, cell viability remained high at 1.11 × 106 CFU/mL. Incubation of encapsulated cells in the presence of 0.6% (w/v) bile salts showed it was able to survive (~104 CFU/mL) after 2 h. Microencapsulated L. lactis retained a higher viability, at ~107 CFU/mL, when incorporated into yogurt compared to non-microencapsulated cells ~105 CFU/mL. The fortification of microencapsulated and non-microencapsulated L. lactis in yogurts influenced the viable cell counts of yogurt starter cultures, Lactobacillus delbrueckii subs. bulgaricus and Streptococcus thermophilus. |
| first_indexed | 2025-11-15T09:41:21Z |
| format | Article |
| id | upm-38401 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:41:21Z |
| publishDate | 2019 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-384012020-05-04T16:28:37Z http://psasir.upm.edu.my/id/eprint/38401/ Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt Fazilah, Nurul Farhana Hamidon, Nurmelissa Hanani Ariff, Arbakariya Khayat, Mohd Ezuan Wasoh @ Mohamad Isa, Helmi Halim, Murni There has been an explosion of probiotic incorporated based product. However, many reports indicated that most of the probiotics have failed to survive in high quantity, which has limited their effectiveness in most functional foods. Thus, to overcome this problem, microencapsulation is considered to be a promising process. In this study, Lactococcus lactis Gh1 was encapsulated via spray-drying with gum Arabic together with Synsepalum dulcificum or commonly known as miracle fruit. It was observed that after spray-drying, high viability (~109 CFU/mL) powders containing L. lactis in combination with S. dulcificum were developed, which was then formulated into yogurt. The tolerance of encapsulated bacterial cells in simulated gastric juice at pH 1.5 was tested in an in-vitro model and the result showed that after 2 h, cell viability remained high at 1.11 × 106 CFU/mL. Incubation of encapsulated cells in the presence of 0.6% (w/v) bile salts showed it was able to survive (~104 CFU/mL) after 2 h. Microencapsulated L. lactis retained a higher viability, at ~107 CFU/mL, when incorporated into yogurt compared to non-microencapsulated cells ~105 CFU/mL. The fortification of microencapsulated and non-microencapsulated L. lactis in yogurts influenced the viable cell counts of yogurt starter cultures, Lactobacillus delbrueckii subs. bulgaricus and Streptococcus thermophilus. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38401/1/38401.pdf Fazilah, Nurul Farhana and Hamidon, Nurmelissa Hanani and Ariff, Arbakariya and Khayat, Mohd Ezuan and Wasoh @ Mohamad Isa, Helmi and Halim, Murni (2019) Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt. Molecules, 24 (7). art. no. 1422. pp. 1-21. ISSN 1420-3049 https://www.mdpi.com/1420-3049/24/7/1422 10.3390/molecules24071422 |
| spellingShingle | Fazilah, Nurul Farhana Hamidon, Nurmelissa Hanani Ariff, Arbakariya Khayat, Mohd Ezuan Wasoh @ Mohamad Isa, Helmi Halim, Murni Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt |
| title | Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt |
| title_full | Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt |
| title_fullStr | Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt |
| title_full_unstemmed | Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt |
| title_short | Microencapsulation of Lactococcus lactis Gh1 with gum Arabic and Synsepalum dulcificum via spray drying for potential inclusion in functional yogurt |
| title_sort | microencapsulation of lactococcus lactis gh1 with gum arabic and synsepalum dulcificum via spray drying for potential inclusion in functional yogurt |
| url | http://psasir.upm.edu.my/id/eprint/38401/ http://psasir.upm.edu.my/id/eprint/38401/ http://psasir.upm.edu.my/id/eprint/38401/ http://psasir.upm.edu.my/id/eprint/38401/1/38401.pdf |