(+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense
In our previous study, two known piperidine alkaloids (+)-spectaline (1) and iso-6-spectaline (2) were isolated from the leaves of Senna spectabilis and showed no toxic effect on L6 cells. In view of the potential use of piperidine alkaloids in S. spectabilis for the treatment of sleeping sickness,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38336/ http://psasir.upm.edu.my/id/eprint/38336/1/38336.pdf |
| _version_ | 1848848851845251072 |
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| author | Lim, Kah Tee Yeoh, Chiann Ying Zainuddin, Zafarina Adenan, Mohd Ilham |
| author_facet | Lim, Kah Tee Yeoh, Chiann Ying Zainuddin, Zafarina Adenan, Mohd Ilham |
| author_sort | Lim, Kah Tee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In our previous study, two known piperidine alkaloids (+)-spectaline (1) and iso-6-spectaline (2) were isolated from the leaves of Senna spectabilis and showed no toxic effect on L6 cells. In view of the potential use of piperidine alkaloids in S. spectabilis for the treatment of sleeping sickness, further investigation on the cell death actions of the parasite after treatment with compound 1 and 2 suggested that the treated parasites died by a process of autophagy based on the characteristic morphological alterations observed in intracellular T. b. rhodesiense. In search for apoptosis, interestingly, trypanosomes treated with high concentration of compound 1 and 2 after 72 h significantly induced an early apoptosis-like programmed cell death (PCD) such as phosphatidylserine (PS) exposure, loss of mitochondrial membrane potential and caspases activation. No DNA laddering discriminated late apoptosis event. Taken together, these findings demonstrated the potential of compound 1 and 2 as a natural chemotherapeutic capable of inducing a possible cross-talk between autophagy and apoptosis in T. b. rhodesiense. |
| first_indexed | 2025-11-15T09:41:04Z |
| format | Article |
| id | upm-38336 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:41:04Z |
| publishDate | 2019 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-383362020-05-04T16:18:54Z http://psasir.upm.edu.my/id/eprint/38336/ (+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense Lim, Kah Tee Yeoh, Chiann Ying Zainuddin, Zafarina Adenan, Mohd Ilham In our previous study, two known piperidine alkaloids (+)-spectaline (1) and iso-6-spectaline (2) were isolated from the leaves of Senna spectabilis and showed no toxic effect on L6 cells. In view of the potential use of piperidine alkaloids in S. spectabilis for the treatment of sleeping sickness, further investigation on the cell death actions of the parasite after treatment with compound 1 and 2 suggested that the treated parasites died by a process of autophagy based on the characteristic morphological alterations observed in intracellular T. b. rhodesiense. In search for apoptosis, interestingly, trypanosomes treated with high concentration of compound 1 and 2 after 72 h significantly induced an early apoptosis-like programmed cell death (PCD) such as phosphatidylserine (PS) exposure, loss of mitochondrial membrane potential and caspases activation. No DNA laddering discriminated late apoptosis event. Taken together, these findings demonstrated the potential of compound 1 and 2 as a natural chemotherapeutic capable of inducing a possible cross-talk between autophagy and apoptosis in T. b. rhodesiense. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38336/1/38336.pdf Lim, Kah Tee and Yeoh, Chiann Ying and Zainuddin, Zafarina and Adenan, Mohd Ilham (2019) (+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense. Tropical Medicine and Infectious Disease, 4 (3). art. no. 98. pp. 1-10. ISSN 2414-6366 https://www.mdpi.com/2414-6366/4/3/98 10.3390/tropicalmed4030098 |
| spellingShingle | Lim, Kah Tee Yeoh, Chiann Ying Zainuddin, Zafarina Adenan, Mohd Ilham (+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense |
| title | (+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense |
| title_full | (+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense |
| title_fullStr | (+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense |
| title_full_unstemmed | (+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense |
| title_short | (+)-Spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in Trypanosoma brucei rhodesiense |
| title_sort | (+)-spectaline and iso-6-spectaline induce a possible cross-talk between autophagy and apoptosis in trypanosoma brucei rhodesiense |
| url | http://psasir.upm.edu.my/id/eprint/38336/ http://psasir.upm.edu.my/id/eprint/38336/ http://psasir.upm.edu.my/id/eprint/38336/ http://psasir.upm.edu.my/id/eprint/38336/1/38336.pdf |