Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds
Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats were randomly divided...
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38172/ http://psasir.upm.edu.my/id/eprint/38172/1/38172.pdf |
| _version_ | 1848848805251776512 |
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| author | Guo, Hui Fang Abd Hamid @ Abdul Razak, Roslida Mohd Ali, Razana Chang, Sui Kiat Rahman, Mohammed Habibur Zainal, Zaida Khaza'ai, Huzwah |
| author_facet | Guo, Hui Fang Abd Hamid @ Abdul Razak, Roslida Mohd Ali, Razana Chang, Sui Kiat Rahman, Mohammed Habibur Zainal, Zaida Khaza'ai, Huzwah |
| author_sort | Guo, Hui Fang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats were randomly divided into six groups of six each and were: untreated control, treated with Silverdin® cream, base cream, base cream with c% EGF, base cream with 3% TRF or base cream with c% EGF and 3% TRF, respectively. Creams were applied once daily for 21 consecutive days. Six animals from each group were sacrificed using anaesthetic overdose on the third, seventh, 11th, 14th and 21st day post-burn. Skin tissues with the wound to be examined were excised for macroscopic and microscopic evaluation and biochemical analyses. Results: EGF + TRF formulation decreased the number of neutrophils, lymphocytes and myofibroblasts post-burn. However, no effects on the number of adipose cells in the healing process were recorded. In addition, lipid peroxidation and nitrite production were found to be reduced post-burn, reducing oxidative stress. Conclusions: Results of the present study indicate that the addition of EGF with TRF have ameliorating effects on deep-partial thickness burn healing parameters. |
| first_indexed | 2025-11-15T09:40:20Z |
| format | Article |
| id | upm-38172 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:40:20Z |
| publishDate | 2020 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-381722020-05-03T23:02:51Z http://psasir.upm.edu.my/id/eprint/38172/ Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds Guo, Hui Fang Abd Hamid @ Abdul Razak, Roslida Mohd Ali, Razana Chang, Sui Kiat Rahman, Mohammed Habibur Zainal, Zaida Khaza'ai, Huzwah Background: An experimental study was undertaken to determine the efficacy of the epidermal growth factor (EGF) with tocotrienol-rich fraction (TRF) cream in the wound-healing process on skin with deep partial-thickness burn in rats. Methods: A total of 180 Sprague-Dawley rats were randomly divided into six groups of six each and were: untreated control, treated with Silverdin® cream, base cream, base cream with c% EGF, base cream with 3% TRF or base cream with c% EGF and 3% TRF, respectively. Creams were applied once daily for 21 consecutive days. Six animals from each group were sacrificed using anaesthetic overdose on the third, seventh, 11th, 14th and 21st day post-burn. Skin tissues with the wound to be examined were excised for macroscopic and microscopic evaluation and biochemical analyses. Results: EGF + TRF formulation decreased the number of neutrophils, lymphocytes and myofibroblasts post-burn. However, no effects on the number of adipose cells in the healing process were recorded. In addition, lipid peroxidation and nitrite production were found to be reduced post-burn, reducing oxidative stress. Conclusions: Results of the present study indicate that the addition of EGF with TRF have ameliorating effects on deep-partial thickness burn healing parameters. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38172/1/38172.pdf Guo, Hui Fang and Abd Hamid @ Abdul Razak, Roslida and Mohd Ali, Razana and Chang, Sui Kiat and Rahman, Mohammed Habibur and Zainal, Zaida and Khaza'ai, Huzwah (2020) Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds. Antioxidants, 9 (2). art. no. 130. pp. 1-17. ISSN 2076-3921 https://www.mdpi.com/2076-3921/9/2/130 10.3390/antiox9020130 |
| spellingShingle | Guo, Hui Fang Abd Hamid @ Abdul Razak, Roslida Mohd Ali, Razana Chang, Sui Kiat Rahman, Mohammed Habibur Zainal, Zaida Khaza'ai, Huzwah Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds |
| title | Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds |
| title_full | Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds |
| title_fullStr | Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds |
| title_full_unstemmed | Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds |
| title_short | Healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds |
| title_sort | healing properties of epidermal growth factor and tocotrienol-rich fraction in deep partial-thickness experimental burn wounds |
| url | http://psasir.upm.edu.my/id/eprint/38172/ http://psasir.upm.edu.my/id/eprint/38172/ http://psasir.upm.edu.my/id/eprint/38172/ http://psasir.upm.edu.my/id/eprint/38172/1/38172.pdf |