Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR
Pineapple (Ananas comosus) waste is a promising source of metabolites for therapeutics, functional foods, and cosmeceutical applications. This study strives to characterize the complete metabolite profiles of a variety of MD2 pineapple waste extracts. Metabolomics strategies were utilized to identif...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38171/ http://psasir.upm.edu.my/id/eprint/38171/1/38171.pdf |
| _version_ | 1848848805011652608 |
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| author | Azizan, Awanis Lee, Ai Xin Abdul Hamid, Nur Ashikin Maulidiani, Maulidiani Mediani, Ahmed Abdul Ghafar, Siti Zulaikha Zolkeflee, Nur Khaleeda Zulaikha Abas, Faridah |
| author_facet | Azizan, Awanis Lee, Ai Xin Abdul Hamid, Nur Ashikin Maulidiani, Maulidiani Mediani, Ahmed Abdul Ghafar, Siti Zulaikha Zolkeflee, Nur Khaleeda Zulaikha Abas, Faridah |
| author_sort | Azizan, Awanis |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pineapple (Ananas comosus) waste is a promising source of metabolites for therapeutics, functional foods, and cosmeceutical applications. This study strives to characterize the complete metabolite profiles of a variety of MD2 pineapple waste extracts. Metabolomics strategies were utilized to identify bioactive metabolites of this variety prepared with different solvent ratios. Each pineapple waste extract was first screened for total phenolic content, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, nitric oxide scavenging, and α-glucosidase inhibitory activities. The highest TPC was found in all samples of the peel, crown, and core extracted using a 50% ethanol ratio, even though the results were fairly significant than those obtained for other ethanol ratios. Additionally, crown extracted with a 100% ethanol ratio demonstrated the highest potency in DPPH and NO scavenging activity, with IC50 values of 296.31 and 338.52 µg/mL, respectively. Peel extracted with 100% ethanol exhibited the highest α-glucosidase inhibitory activity with an IC50 value of 92.95 µg/mL. Then, the extracts were analyzed and the data from 1H NMR were processed using multivariate data analysis. A partial least squares and correlogram plot suggested that 3-methylglutaric acid, threonine, valine, and α-linolenic acid were the main contributors to the antioxidant activities, whereas epicatechin was responsible for the α-glucosidase inhibitory activity. Relative quantification further supported that 100% crown extract was among the extracts that possessed the most abundant potential metabolites. The present study demonstrated that the crown and peel parts of MD2 pineapple extracted with 100% ethanol are potentially natural sources of antioxidants and α-glucosidase inhibitors, respectively. |
| first_indexed | 2025-11-15T09:40:20Z |
| format | Article |
| id | upm-38171 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:40:20Z |
| publishDate | 2020 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-381712020-05-03T23:02:42Z http://psasir.upm.edu.my/id/eprint/38171/ Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR Azizan, Awanis Lee, Ai Xin Abdul Hamid, Nur Ashikin Maulidiani, Maulidiani Mediani, Ahmed Abdul Ghafar, Siti Zulaikha Zolkeflee, Nur Khaleeda Zulaikha Abas, Faridah Pineapple (Ananas comosus) waste is a promising source of metabolites for therapeutics, functional foods, and cosmeceutical applications. This study strives to characterize the complete metabolite profiles of a variety of MD2 pineapple waste extracts. Metabolomics strategies were utilized to identify bioactive metabolites of this variety prepared with different solvent ratios. Each pineapple waste extract was first screened for total phenolic content, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, nitric oxide scavenging, and α-glucosidase inhibitory activities. The highest TPC was found in all samples of the peel, crown, and core extracted using a 50% ethanol ratio, even though the results were fairly significant than those obtained for other ethanol ratios. Additionally, crown extracted with a 100% ethanol ratio demonstrated the highest potency in DPPH and NO scavenging activity, with IC50 values of 296.31 and 338.52 µg/mL, respectively. Peel extracted with 100% ethanol exhibited the highest α-glucosidase inhibitory activity with an IC50 value of 92.95 µg/mL. Then, the extracts were analyzed and the data from 1H NMR were processed using multivariate data analysis. A partial least squares and correlogram plot suggested that 3-methylglutaric acid, threonine, valine, and α-linolenic acid were the main contributors to the antioxidant activities, whereas epicatechin was responsible for the α-glucosidase inhibitory activity. Relative quantification further supported that 100% crown extract was among the extracts that possessed the most abundant potential metabolites. The present study demonstrated that the crown and peel parts of MD2 pineapple extracted with 100% ethanol are potentially natural sources of antioxidants and α-glucosidase inhibitors, respectively. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38171/1/38171.pdf Azizan, Awanis and Lee, Ai Xin and Abdul Hamid, Nur Ashikin and Maulidiani, Maulidiani and Mediani, Ahmed and Abdul Ghafar, Siti Zulaikha and Zolkeflee, Nur Khaleeda Zulaikha and Abas, Faridah (2020) Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR. Foods, 9 (2). art. no. 173. pp. 1-19. ISSN 2304-8158 https://www.mdpi.com/2304-8158/9/2/173 10.3390/foods9020173 |
| spellingShingle | Azizan, Awanis Lee, Ai Xin Abdul Hamid, Nur Ashikin Maulidiani, Maulidiani Mediani, Ahmed Abdul Ghafar, Siti Zulaikha Zolkeflee, Nur Khaleeda Zulaikha Abas, Faridah Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR |
| title | Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR |
| title_full | Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR |
| title_fullStr | Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR |
| title_full_unstemmed | Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR |
| title_short | Potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1H NMR |
| title_sort | potentially bioactive metabolites from pineapple waste extracts and their antioxidant and α-glucosidase inhibitory activities by 1h nmr |
| url | http://psasir.upm.edu.my/id/eprint/38171/ http://psasir.upm.edu.my/id/eprint/38171/ http://psasir.upm.edu.my/id/eprint/38171/ http://psasir.upm.edu.my/id/eprint/38171/1/38171.pdf |