Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae)
The effect of batch and fed-batch fermentation on the cultivation performance of Bacillus thuringiensis was investigated using a 5-l stirred tank bioreactor. Significantly higher viable cell count (> 1.5 × 1012 CFU/ml) was obtained in the fed-batch compared to batch fermentation (1.4 × 1012 CFU/m...
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| Format: | Article |
| Language: | English |
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Egyptian Society for Biological Control of Pests
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38100/ http://psasir.upm.edu.my/id/eprint/38100/1/38100.pdf |
| _version_ | 1848848784030695424 |
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| author | Mohd Masri, Mohamed Mazmira Ariff, Arbakariya |
| author_facet | Mohd Masri, Mohamed Mazmira Ariff, Arbakariya |
| author_sort | Mohd Masri, Mohamed Mazmira |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The effect of batch and fed-batch fermentation on the cultivation performance of Bacillus thuringiensis was investigated using a 5-l stirred tank bioreactor. Significantly higher viable cell count (> 1.5 × 1012 CFU/ml) was obtained in the fed-batch compared to batch fermentation (1.4 × 1012 CFU/ml). Glucose feeding during the fermentation seemed to enhance cell growth but failed to enhance the sporulation rate. It was found that sporulation and δ-endotoxin synthesis in fed-batch fermentation could be enhanced by the application of optimal dissolved oxygen tension (DOT) control strategy without affecting the cell growth. Fed-batch cultivation with feeding at the exponential growth phase where the DOT was switched from 80 to 40% at 12 h of cultivation recorded the highest spore count of 7.1 × 1011 spore/ml. Cultures obtained from batch cultivation, as well as fed-batch cultivation with feeding at lag or exponential growth phase and the application of optimal DOT control strategy, recorded the presence of δ-endotoxin; however, none was detected in intermittent fed-batch fermentation. Bioassay data against the bagworm Metisa plana Walker (Lepidoptera: Psychidae) recorded the highest corrected mortality (80%) at 7 days of treatment (DAT), using the culture obtained from fed-batch cultivation with feeding during the exponential growth phase, and the DOT was switched from 80 to 40% at 12 h of cultivation. It is important to note that all cultures containing δ-endotoxin exhibited 100% mortality towards M. plana at 14 DAT. |
| first_indexed | 2025-11-15T09:40:00Z |
| format | Article |
| id | upm-38100 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:40:00Z |
| publishDate | 2020 |
| publisher | Egyptian Society for Biological Control of Pests |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-381002020-04-14T13:59:41Z http://psasir.upm.edu.my/id/eprint/38100/ Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae) Mohd Masri, Mohamed Mazmira Ariff, Arbakariya The effect of batch and fed-batch fermentation on the cultivation performance of Bacillus thuringiensis was investigated using a 5-l stirred tank bioreactor. Significantly higher viable cell count (> 1.5 × 1012 CFU/ml) was obtained in the fed-batch compared to batch fermentation (1.4 × 1012 CFU/ml). Glucose feeding during the fermentation seemed to enhance cell growth but failed to enhance the sporulation rate. It was found that sporulation and δ-endotoxin synthesis in fed-batch fermentation could be enhanced by the application of optimal dissolved oxygen tension (DOT) control strategy without affecting the cell growth. Fed-batch cultivation with feeding at the exponential growth phase where the DOT was switched from 80 to 40% at 12 h of cultivation recorded the highest spore count of 7.1 × 1011 spore/ml. Cultures obtained from batch cultivation, as well as fed-batch cultivation with feeding at lag or exponential growth phase and the application of optimal DOT control strategy, recorded the presence of δ-endotoxin; however, none was detected in intermittent fed-batch fermentation. Bioassay data against the bagworm Metisa plana Walker (Lepidoptera: Psychidae) recorded the highest corrected mortality (80%) at 7 days of treatment (DAT), using the culture obtained from fed-batch cultivation with feeding during the exponential growth phase, and the DOT was switched from 80 to 40% at 12 h of cultivation. It is important to note that all cultures containing δ-endotoxin exhibited 100% mortality towards M. plana at 14 DAT. Egyptian Society for Biological Control of Pests 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38100/1/38100.pdf Mohd Masri, Mohamed Mazmira and Ariff, Arbakariya (2020) Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae). Egyptian Journal of Biological Pest Control, 30. art. no. 2. pp. 1-10. ISSN 1110-1768; ESSN: 2536-9342 https://link.springer.com/article/10.1186/s41938-020-0204-y 10.1186/s41938-020-0204-y |
| spellingShingle | Mohd Masri, Mohamed Mazmira Ariff, Arbakariya Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae) |
| title | Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae) |
| title_full | Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae) |
| title_fullStr | Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae) |
| title_full_unstemmed | Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae) |
| title_short | Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae) |
| title_sort | effect of different fermentation strategies on bacillus thuringiensis cultivation and its toxicity towards the bagworm, metisa plana walker (lepidoptera: psychidae) |
| url | http://psasir.upm.edu.my/id/eprint/38100/ http://psasir.upm.edu.my/id/eprint/38100/ http://psasir.upm.edu.my/id/eprint/38100/ http://psasir.upm.edu.my/id/eprint/38100/1/38100.pdf |