Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode
Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graph...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/38098/ http://psasir.upm.edu.my/id/eprint/38098/1/38098.pdf |
| _version_ | 1848848783452930048 |
|---|---|
| author | A. Talib, Nurul Ain Salam, Faridah Sulaiman, Yusran |
| author_facet | A. Talib, Nurul Ain Salam, Faridah Sulaiman, Yusran |
| author_sort | A. Talib, Nurul Ain |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA). |
| first_indexed | 2025-11-15T09:39:59Z |
| format | Article |
| id | upm-38098 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:39:59Z |
| publishDate | 2018 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-380982020-04-14T14:01:22Z http://psasir.upm.edu.my/id/eprint/38098/ Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode A. Talib, Nurul Ain Salam, Faridah Sulaiman, Yusran Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA). MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38098/1/38098.pdf A. Talib, Nurul Ain and Salam, Faridah and Sulaiman, Yusran (2018) Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode. Sensors, 18 (12). art. no. 4324. pp. 1-13. ISSN 424-8220 https://www.mdpi.com/1424-8220/18/12/4324 10.3390/s18124324 |
| spellingShingle | A. Talib, Nurul Ain Salam, Faridah Sulaiman, Yusran Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode |
| title | Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode |
| title_full | Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode |
| title_fullStr | Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode |
| title_full_unstemmed | Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode |
| title_short | Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode |
| title_sort | development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode |
| url | http://psasir.upm.edu.my/id/eprint/38098/ http://psasir.upm.edu.my/id/eprint/38098/ http://psasir.upm.edu.my/id/eprint/38098/ http://psasir.upm.edu.my/id/eprint/38098/1/38098.pdf |