Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers
Oil palm breeding has been progressing very well in Southeast Asia, especially in Malaysia and Indonesia. Despite this progress, there are still problems due to the difficulty of controlled crossing in oil palm. Contaminated/illegitimate progeny has appeared in some breeding programs; late and failu...
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| Format: | Article |
| Language: | English |
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Springer Link
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37928/ http://psasir.upm.edu.my/id/eprint/37928/1/Illegitimacy%20and%20sibship%20assignments%20in%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20half-sib%20families%20using%20single%20locus%20DNA%20microsatellite%20markers.pdf |
| _version_ | 1848848738579120128 |
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| author | Hama-Ali, Emad Omer Syed Alwee, Sharifah Shahrul Rabiah Tan, Soon Guan Panandam, Jothi Malar Ho, Chai Ling Namasivayam, Parameswari Hoh, Boon Peng |
| author_facet | Hama-Ali, Emad Omer Syed Alwee, Sharifah Shahrul Rabiah Tan, Soon Guan Panandam, Jothi Malar Ho, Chai Ling Namasivayam, Parameswari Hoh, Boon Peng |
| author_sort | Hama-Ali, Emad Omer |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Oil palm breeding has been progressing very well in Southeast Asia, especially in Malaysia and Indonesia. Despite this progress, there are still problems due to the difficulty of controlled crossing in oil palm. Contaminated/illegitimate progeny has appeared in some breeding programs; late and failure of detection by the traditional method causes a waste of time and labor. The use of molecular markers improves the integrity of breeding programs in perennial crops such as oil palm. Four half-sib families with a total of 200 progeny were used in this study. Thirty polymorphic single locus DNA microsatellites markers were typed to identify the illegitimate individuals and to obtain the correct parental and progeny assignments by using the CERVUS and COLONY programs. Three illegitimate palms (1.5 %) were found, and 16 loci proved to be sufficient for sibship assignments without parental genotypes by using the COLONY program. The pairwise-likelihood score (PLS) method was better for half-sib family assignments than the full likelihood (FL) method. |
| first_indexed | 2025-11-15T09:39:16Z |
| format | Article |
| id | upm-37928 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:39:16Z |
| publishDate | 2015 |
| publisher | Springer Link |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-379282015-12-29T10:56:20Z http://psasir.upm.edu.my/id/eprint/37928/ Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers Hama-Ali, Emad Omer Syed Alwee, Sharifah Shahrul Rabiah Tan, Soon Guan Panandam, Jothi Malar Ho, Chai Ling Namasivayam, Parameswari Hoh, Boon Peng Oil palm breeding has been progressing very well in Southeast Asia, especially in Malaysia and Indonesia. Despite this progress, there are still problems due to the difficulty of controlled crossing in oil palm. Contaminated/illegitimate progeny has appeared in some breeding programs; late and failure of detection by the traditional method causes a waste of time and labor. The use of molecular markers improves the integrity of breeding programs in perennial crops such as oil palm. Four half-sib families with a total of 200 progeny were used in this study. Thirty polymorphic single locus DNA microsatellites markers were typed to identify the illegitimate individuals and to obtain the correct parental and progeny assignments by using the CERVUS and COLONY programs. Three illegitimate palms (1.5 %) were found, and 16 loci proved to be sufficient for sibship assignments without parental genotypes by using the COLONY program. The pairwise-likelihood score (PLS) method was better for half-sib family assignments than the full likelihood (FL) method. Springer Link 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37928/1/Illegitimacy%20and%20sibship%20assignments%20in%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20half-sib%20families%20using%20single%20locus%20DNA%20microsatellite%20markers.pdf Hama-Ali, Emad Omer and Syed Alwee, Sharifah Shahrul Rabiah and Tan, Soon Guan and Panandam, Jothi Malar and Ho, Chai Ling and Namasivayam, Parameswari and Hoh, Boon Peng (2015) Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers. Molecular Biology Reports, 42 (5). pp. 917-925. ISSN 0301-4851; ESSN:1573-4978 http://link.springer.com/article/10.1007%2Fs11033-014-3829-7 10.1007/s11033-014-3829-7 |
| spellingShingle | Hama-Ali, Emad Omer Syed Alwee, Sharifah Shahrul Rabiah Tan, Soon Guan Panandam, Jothi Malar Ho, Chai Ling Namasivayam, Parameswari Hoh, Boon Peng Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers |
| title | Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers |
| title_full | Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers |
| title_fullStr | Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers |
| title_full_unstemmed | Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers |
| title_short | Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers |
| title_sort | illegitimacy and sibship assignments in oil palm (elaeis guineensis jacq.) half-sib families using single locus dna microsatellite markers |
| url | http://psasir.upm.edu.my/id/eprint/37928/ http://psasir.upm.edu.my/id/eprint/37928/ http://psasir.upm.edu.my/id/eprint/37928/ http://psasir.upm.edu.my/id/eprint/37928/1/Illegitimacy%20and%20sibship%20assignments%20in%20oil%20palm%20%28Elaeis%20guineensis%20Jacq.%29%20half-sib%20families%20using%20single%20locus%20DNA%20microsatellite%20markers.pdf |