Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes
Caseous lymphadenitis is a chronic disease characterized by internal and external abscesses and is caused by Corynebacterium pseudotuberculosis.Thisstudy is designed to measure the hematological, biochemicaland serum electrolyte changes in experimental non-pregnant doesinoculated with Corynebacteriu...
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| Format: | Article |
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International Organization of Scientific Research
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37818/ |
| _version_ | 1848848708374888448 |
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| author | Othman, Abas Mazni Abdullah, Faez Firdaus Jesse Mohd Nordi, Nur Adza Rina Ilyasu, Yusuf Saad, Mohd Zamri Haron, Abd Wahid Saharee, Abdul Aziz Mohd Lila, Mohd Azmi |
| author_facet | Othman, Abas Mazni Abdullah, Faez Firdaus Jesse Mohd Nordi, Nur Adza Rina Ilyasu, Yusuf Saad, Mohd Zamri Haron, Abd Wahid Saharee, Abdul Aziz Mohd Lila, Mohd Azmi |
| author_sort | Othman, Abas Mazni |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Caseous lymphadenitis is a chronic disease characterized by internal and external abscesses and is caused by Corynebacterium pseudotuberculosis.Thisstudy is designed to measure the hematological, biochemicaland serum electrolyte changes in experimental non-pregnant doesinoculated with Corynebacterium pseudotuberculosis via variousroutes. Little is known about the changes in these parameters through different routes of infection.A total of 20 healthy does (n=20) were divided into 4 groups (intradermal, intranasal, oral and control) of 5 goats each. The three groups were inoculated with 107cfu/1ml of live Corynebacterium pseudotuberculosis, while control is kept unexposed. Following infection, blood samples were collected from the jugular vein for hematological, biochemical and serum electrolyteanalysis. A significantdecrease was observed in RBC count (p<0.05) in the intradermal group, whilenochangesobserved in PCV, Hb, MCV and MCHC parameters. Significant increasein WBC were observed in intradermal, intranasal and oral groups (p<0.05). Slight increase (p<0.05) in monocyte count was observed in intranasal group. A significant reduction (p<0.05) in lymphocytic count was observed for the intranasally inoculated group (p<0.05) and a slight increase (p<0.05) in neutrophil was observed in intranasal and intradermal groups. Biochemically,creatinine and albumin levels increases inintranasal group(p<0.05) and GGT levels were elevated in all the three infected groups (p<0.05).However,there were no significant changes in AST, T.Protein and APT. Serumelectrolyte, revealed a decrease in Calcium (Ca+) concentration in intradermal group with concentration of 2.22mmol/L,intranasal 2.23mmol/L group (p<0.05), and no changes were observed in potassium (K+) and sodium (Na+). The study, therefore, observed increase in WBCs, neutrophils, monocytes, creatinine, GGT, albumin levels and decrease in RBCs, lymphocytes and calcium concentrations on different route of infections. |
| first_indexed | 2025-11-15T09:38:48Z |
| format | Article |
| id | upm-37818 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:38:48Z |
| publishDate | 2014 |
| publisher | International Organization of Scientific Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-378182015-09-18T11:18:15Z http://psasir.upm.edu.my/id/eprint/37818/ Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes Othman, Abas Mazni Abdullah, Faez Firdaus Jesse Mohd Nordi, Nur Adza Rina Ilyasu, Yusuf Saad, Mohd Zamri Haron, Abd Wahid Saharee, Abdul Aziz Mohd Lila, Mohd Azmi Caseous lymphadenitis is a chronic disease characterized by internal and external abscesses and is caused by Corynebacterium pseudotuberculosis.Thisstudy is designed to measure the hematological, biochemicaland serum electrolyte changes in experimental non-pregnant doesinoculated with Corynebacterium pseudotuberculosis via variousroutes. Little is known about the changes in these parameters through different routes of infection.A total of 20 healthy does (n=20) were divided into 4 groups (intradermal, intranasal, oral and control) of 5 goats each. The three groups were inoculated with 107cfu/1ml of live Corynebacterium pseudotuberculosis, while control is kept unexposed. Following infection, blood samples were collected from the jugular vein for hematological, biochemical and serum electrolyteanalysis. A significantdecrease was observed in RBC count (p<0.05) in the intradermal group, whilenochangesobserved in PCV, Hb, MCV and MCHC parameters. Significant increasein WBC were observed in intradermal, intranasal and oral groups (p<0.05). Slight increase (p<0.05) in monocyte count was observed in intranasal group. A significant reduction (p<0.05) in lymphocytic count was observed for the intranasally inoculated group (p<0.05) and a slight increase (p<0.05) in neutrophil was observed in intranasal and intradermal groups. Biochemically,creatinine and albumin levels increases inintranasal group(p<0.05) and GGT levels were elevated in all the three infected groups (p<0.05).However,there were no significant changes in AST, T.Protein and APT. Serumelectrolyte, revealed a decrease in Calcium (Ca+) concentration in intradermal group with concentration of 2.22mmol/L,intranasal 2.23mmol/L group (p<0.05), and no changes were observed in potassium (K+) and sodium (Na+). The study, therefore, observed increase in WBCs, neutrophils, monocytes, creatinine, GGT, albumin levels and decrease in RBCs, lymphocytes and calcium concentrations on different route of infections. International Organization of Scientific Research 2014-10 Article PeerReviewed Othman, Abas Mazni and Abdullah, Faez Firdaus Jesse and Mohd Nordi, Nur Adza Rina and Ilyasu, Yusuf and Saad, Mohd Zamri and Haron, Abd Wahid and Saharee, Abdul Aziz and Mohd Lila, Mohd Azmi (2014) Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes. IOSR Journal of Agriculture and Veterinary Science, 7 (10 ver.2). pp. 5-8. ISSN 2319-2372; ESSN: 2319-2380 http://www.iosrjournals.org/iosr-javs/pages/7%2810%29Version-2.html 10.9790/2380-071020508 |
| spellingShingle | Othman, Abas Mazni Abdullah, Faez Firdaus Jesse Mohd Nordi, Nur Adza Rina Ilyasu, Yusuf Saad, Mohd Zamri Haron, Abd Wahid Saharee, Abdul Aziz Mohd Lila, Mohd Azmi Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes |
| title | Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes |
| title_full | Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes |
| title_fullStr | Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes |
| title_full_unstemmed | Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes |
| title_short | Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes |
| title_sort | haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with corynebacterium pseudotuberculosis via various routes |
| url | http://psasir.upm.edu.my/id/eprint/37818/ http://psasir.upm.edu.my/id/eprint/37818/ http://psasir.upm.edu.my/id/eprint/37818/ |