Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources
This study characterized carriage and clinical pneumococcal isolates for serotypes, penicillin susceptibility, virulence genes and restriction fragment length polymorphism (RFLP) pattern of penicillin binding protein (PBP) genes. DNA fingerprint of isolates was generated by BOX-PCR. Majority of sero...
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| Format: | Article |
| Language: | English |
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Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37766/ http://psasir.upm.edu.my/id/eprint/37766/1/37766.pdf |
| _version_ | 1848848695303340032 |
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| author | Mohamed Shakrina, Nik Noorul Shakira Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Mohd Desa, Mohd Nasir |
| author_facet | Mohamed Shakrina, Nik Noorul Shakira Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Mohd Desa, Mohd Nasir |
| author_sort | Mohamed Shakrina, Nik Noorul Shakira |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study characterized carriage and clinical pneumococcal isolates for serotypes, penicillin susceptibility, virulence genes and restriction fragment length polymorphism (RFLP) pattern of penicillin binding protein (PBP) genes. DNA fingerprint of isolates was generated by BOX-PCR. Majority of serotypes were 23F followed by 19F, 19A and 6A. Twenty-four percent of isolates were penicillin non-susceptible (PNSP). All of the targeted virulence genes were detected in all isolates with the exception of pili; 20.6% (n = 22) for PI-1 and 14.0% (n = 15) for PI-2. Of the 13 isolates which carried both PI-1 and PI-2, 10 were of clinical origin. Digested pbp-DNA produced three PBP-RFLP profiles for pbp1a (A1 to A3), six profiles for pbp2b (B1 to B6) and seven for pbp2x (X1 to X7) mostly in PNSPs. Based on BOX-PCR analysis, the majority of isolates were genetically diverse with a small number of potentially related isolates carrying pili genes. No obvious genotypic association was observed pertaining to carriage and clinical origin of isolates. |
| first_indexed | 2025-11-15T09:38:35Z |
| format | Article |
| id | upm-37766 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:38:35Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-377662016-04-22T02:38:39Z http://psasir.upm.edu.my/id/eprint/37766/ Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources Mohamed Shakrina, Nik Noorul Shakira Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Mohd Desa, Mohd Nasir This study characterized carriage and clinical pneumococcal isolates for serotypes, penicillin susceptibility, virulence genes and restriction fragment length polymorphism (RFLP) pattern of penicillin binding protein (PBP) genes. DNA fingerprint of isolates was generated by BOX-PCR. Majority of serotypes were 23F followed by 19F, 19A and 6A. Twenty-four percent of isolates were penicillin non-susceptible (PNSP). All of the targeted virulence genes were detected in all isolates with the exception of pili; 20.6% (n = 22) for PI-1 and 14.0% (n = 15) for PI-2. Of the 13 isolates which carried both PI-1 and PI-2, 10 were of clinical origin. Digested pbp-DNA produced three PBP-RFLP profiles for pbp1a (A1 to A3), six profiles for pbp2b (B1 to B6) and seven for pbp2x (X1 to X7) mostly in PNSPs. Based on BOX-PCR analysis, the majority of isolates were genetically diverse with a small number of potentially related isolates carrying pili genes. No obvious genotypic association was observed pertaining to carriage and clinical origin of isolates. Elsevier 2014-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37766/1/37766.pdf Mohamed Shakrina, Nik Noorul Shakira and Masri, Siti Norbaya and Mohd Taib, Niazlin and Amin Nordin, Syafinaz and Jamal, Farida and Mohd Desa, Mohd Nasir (2014) Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources. Comparative Immunology, Microbiology and Infectious Diseases, 37 (5-6). pp. 347-354. ISSN 0147-9571; ESSN: 1878-1667 http://www.sciencedirect.com/science/article/pii/S0147957114000587?np=y 10.1016/j.cimid.2014.10.005 |
| spellingShingle | Mohamed Shakrina, Nik Noorul Shakira Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Mohd Desa, Mohd Nasir Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources |
| title | Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources |
| title_full | Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources |
| title_fullStr | Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources |
| title_full_unstemmed | Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources |
| title_short | Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources |
| title_sort | genotypic characterization of malaysian human isolates of streptococcus pneumoniae from carriage and clinical sources |
| url | http://psasir.upm.edu.my/id/eprint/37766/ http://psasir.upm.edu.my/id/eprint/37766/ http://psasir.upm.edu.my/id/eprint/37766/ http://psasir.upm.edu.my/id/eprint/37766/1/37766.pdf |