Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs
Existing nonviral gene delivery systems to lungs are inefficient and associated with dose limiting toxicity in mammalian cells. Therefore, carbonate apatite (CO3Ap) nanoparticles were examined as an alternative strategy for effective gene delivery to the lungs. This study aimed to (1) assess the gen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37747/ http://psasir.upm.edu.my/id/eprint/37747/1/646787.pdf |
| _version_ | 1848848690679119872 |
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| author | Alhaji, Suleiman Yusuf Chowdhury, Ezharul Houque Rosli, Rozita Hassan, Fatma Abdullah, Syahrilnizam |
| author_facet | Alhaji, Suleiman Yusuf Chowdhury, Ezharul Houque Rosli, Rozita Hassan, Fatma Abdullah, Syahrilnizam |
| author_sort | Alhaji, Suleiman Yusuf |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Existing nonviral gene delivery systems to lungs are inefficient and associated with dose limiting toxicity in mammalian cells. Therefore, carbonate apatite (CO3Ap) nanoparticles were examined as an alternative strategy for effective gene delivery to the lungs. This study aimed to (1) assess the gene delivery efficiency of CO3Ap in vitro and in mouse lungs, (2) evaluate the cytotoxicity effect of CO3Ap/pDNA in vitro, and (3) characterize the CO3Ap/pDNA complex formulations. A significantly high level of reporter gene expression was detected from the lung cell line transfected with CO3Ap/pDNA complex prepared in both serum and serum-free medium. Cytotoxicity analysis revealed that the percentage of the viable cells treated with CO3Ap to be almost similar to the untreated cells. Characterization analyses showed that the CO3Ap/pDNA complexes are in a nanometer range with aggregated spherical structures and tended to be more negatively charged. In the lung of mice, highest level of transgene expression was observed when CO3Ap (8 μL) was complexed with 40 μg of pDNA at day 1 after administration. Although massive reduction of gene expression was seen beyond day 1 post administration, the level of expression remained significant throughout the study period. |
| first_indexed | 2025-11-15T09:38:31Z |
| format | Article |
| id | upm-37747 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:38:31Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-377472016-04-22T08:39:31Z http://psasir.upm.edu.my/id/eprint/37747/ Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs Alhaji, Suleiman Yusuf Chowdhury, Ezharul Houque Rosli, Rozita Hassan, Fatma Abdullah, Syahrilnizam Existing nonviral gene delivery systems to lungs are inefficient and associated with dose limiting toxicity in mammalian cells. Therefore, carbonate apatite (CO3Ap) nanoparticles were examined as an alternative strategy for effective gene delivery to the lungs. This study aimed to (1) assess the gene delivery efficiency of CO3Ap in vitro and in mouse lungs, (2) evaluate the cytotoxicity effect of CO3Ap/pDNA in vitro, and (3) characterize the CO3Ap/pDNA complex formulations. A significantly high level of reporter gene expression was detected from the lung cell line transfected with CO3Ap/pDNA complex prepared in both serum and serum-free medium. Cytotoxicity analysis revealed that the percentage of the viable cells treated with CO3Ap to be almost similar to the untreated cells. Characterization analyses showed that the CO3Ap/pDNA complexes are in a nanometer range with aggregated spherical structures and tended to be more negatively charged. In the lung of mice, highest level of transgene expression was observed when CO3Ap (8 μL) was complexed with 40 μg of pDNA at day 1 after administration. Although massive reduction of gene expression was seen beyond day 1 post administration, the level of expression remained significant throughout the study period. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37747/1/646787.pdf Alhaji, Suleiman Yusuf and Chowdhury, Ezharul Houque and Rosli, Rozita and Hassan, Fatma and Abdullah, Syahrilnizam (2014) Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs. BioMed Research International, 2014. art. no. 646787. pp. 1-12. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2014/646787/abs/ 10.1155/2014/646787 |
| spellingShingle | Alhaji, Suleiman Yusuf Chowdhury, Ezharul Houque Rosli, Rozita Hassan, Fatma Abdullah, Syahrilnizam Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs |
| title | Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs |
| title_full | Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs |
| title_fullStr | Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs |
| title_full_unstemmed | Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs |
| title_short | Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs |
| title_sort | gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs |
| url | http://psasir.upm.edu.my/id/eprint/37747/ http://psasir.upm.edu.my/id/eprint/37747/ http://psasir.upm.edu.my/id/eprint/37747/ http://psasir.upm.edu.my/id/eprint/37747/1/646787.pdf |