Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles
The present work investigated the antioxidant properties and antihypertensive activity of magnesium orotate (MgOr) using various established in vitro assays, such as β-carotene bleaching activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and nitric oxide scavenging activity as well as angiotensin conve...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37570/ http://psasir.upm.edu.my/id/eprint/37570/1/16%20-%20IFRJ181577.R1.pdf |
| _version_ | 1848848642635464704 |
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| author | Abdelkader, Hassani Hussain, Siti Aslina Abdullah, Norhafizah Kamarudin, Suryani Rosli, Rozita |
| author_facet | Abdelkader, Hassani Hussain, Siti Aslina Abdullah, Norhafizah Kamarudin, Suryani Rosli, Rozita |
| author_sort | Abdelkader, Hassani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present work investigated the antioxidant properties and antihypertensive activity of magnesium orotate (MgOr) using various established in vitro assays, such as β-carotene bleaching activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and nitric oxide scavenging activity as well as angiotensin converting enzyme (ACE) inhibitory activity. Magnesium orotate nanoparticles (MgOrGANPs) were prepared using the gum arabic (GA) as stabiliser coatings for nanoparticles through freeze-drying method. The in vitro cytoxicity of MgOrGANPs against human breast cancer MCF7, liver cancer HepG2, and colon cancer HT29 was investigated. The nitric oxide (NO) and DPPH scavenging assays of MgOrGANPs showed a dose-dependent trend, while 500 and 200 μL/mL were significantly more effective than the other concentrations with an IC50 of 89.56 μg/mL and 63.22% DPPH scavenging capacity respectively. The exposure of human cancer cells to MgOrGANPs at 1.56 – 1,000 μg/mL using 3-)4,5-dimethylthiazol-2-yl(2,5-diphenyl tetrazolium bromide (MTT) inhibited the growth of cell lines examined in a dose-dependent manner. Hence, MgOrGANPs may have great potential to be applied for cancer treatments. |
| first_indexed | 2025-11-15T09:37:45Z |
| format | Article |
| id | upm-37570 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:37:45Z |
| publishDate | 2020 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-375702020-07-06T03:48:13Z http://psasir.upm.edu.my/id/eprint/37570/ Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles Abdelkader, Hassani Hussain, Siti Aslina Abdullah, Norhafizah Kamarudin, Suryani Rosli, Rozita The present work investigated the antioxidant properties and antihypertensive activity of magnesium orotate (MgOr) using various established in vitro assays, such as β-carotene bleaching activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and nitric oxide scavenging activity as well as angiotensin converting enzyme (ACE) inhibitory activity. Magnesium orotate nanoparticles (MgOrGANPs) were prepared using the gum arabic (GA) as stabiliser coatings for nanoparticles through freeze-drying method. The in vitro cytoxicity of MgOrGANPs against human breast cancer MCF7, liver cancer HepG2, and colon cancer HT29 was investigated. The nitric oxide (NO) and DPPH scavenging assays of MgOrGANPs showed a dose-dependent trend, while 500 and 200 μL/mL were significantly more effective than the other concentrations with an IC50 of 89.56 μg/mL and 63.22% DPPH scavenging capacity respectively. The exposure of human cancer cells to MgOrGANPs at 1.56 – 1,000 μg/mL using 3-)4,5-dimethylthiazol-2-yl(2,5-diphenyl tetrazolium bromide (MTT) inhibited the growth of cell lines examined in a dose-dependent manner. Hence, MgOrGANPs may have great potential to be applied for cancer treatments. Faculty of Food Science and Technology, Universiti Putra Malaysia 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/37570/1/16%20-%20IFRJ181577.R1.pdf Abdelkader, Hassani and Hussain, Siti Aslina and Abdullah, Norhafizah and Kamarudin, Suryani and Rosli, Rozita (2020) Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles. International Food Research Journal, 27 (1). pp. 150-159. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/27%20(01)%202020/16%20-%20IFRJ181577.R1.pdf |
| spellingShingle | Abdelkader, Hassani Hussain, Siti Aslina Abdullah, Norhafizah Kamarudin, Suryani Rosli, Rozita Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles |
| title | Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles |
| title_full | Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles |
| title_fullStr | Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles |
| title_full_unstemmed | Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles |
| title_short | Preparation, antioxidant potential and angiotensin converting enzyme (ACE) inhibitory activity of gum Arabic-stabilised magnesium orotate nanoparticles |
| title_sort | preparation, antioxidant potential and angiotensin converting enzyme (ace) inhibitory activity of gum arabic-stabilised magnesium orotate nanoparticles |
| url | http://psasir.upm.edu.my/id/eprint/37570/ http://psasir.upm.edu.my/id/eprint/37570/ http://psasir.upm.edu.my/id/eprint/37570/1/16%20-%20IFRJ181577.R1.pdf |