Engineering the lactococcal mevalonate pathway for increased sesquiterpene production
Isoprenoids are a large, diverse group of secondary metabolites which has recently raised a renewed research interest due to genetic engineering advances, allowing specific isoprenoids to be produced and characterized in heterologous hosts. Many researches on metabolic engineering of heterologous ho...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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John Wiley & Sons
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37450/ http://psasir.upm.edu.my/id/eprint/37450/1/37450.pdf |
| _version_ | 1848848610618245120 |
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| author | Song, Adelene Ai Lian Abdullah, Janna Ong Abdullah, Mohd Puad Shafee, Norazizah Othman, Roohaida Mohd Noor, Normah Abdul Rahim, Raha |
| author_facet | Song, Adelene Ai Lian Abdullah, Janna Ong Abdullah, Mohd Puad Shafee, Norazizah Othman, Roohaida Mohd Noor, Normah Abdul Rahim, Raha |
| author_sort | Song, Adelene Ai Lian |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Isoprenoids are a large, diverse group of secondary metabolites which has recently raised a renewed research interest due to genetic engineering advances, allowing specific isoprenoids to be produced and characterized in heterologous hosts. Many researches on metabolic engineering of heterologous hosts for increased isoprenoid production are focussed on Escherichia coli and yeasts. E. coli, as most prokaryotes, use the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway for isoprenoid production. Yeasts on the other hand, use the mevalonate pathway which is commonly found in eukaryotes. However, Lactococcus lactis is an attractive alternative host for heterologous isoprenoid production. Apart from being food-grade, this Gram-positive prokaryote uses the mevalonate pathway for isoprenoid production instead of the MEP pathway. Previous studies have shown that L. lactis is able to produce sesquiterpenes through heterologous expression of plant sesquiterpene synthases. In this work, we analysed the gene expression of the lactococcal mevalonate pathway through RT-qPCR to successfully engineer L. lactis as an efficient host for isoprenoid production. We then overexpressed the mvk gene singly or co-expressed with the mvaA gene as an attempt to increase β-sesquiphellandrene production in L. lactis. It was observed that co-expression of mvk with mvaA doubled the amount of β-sesquiphellandrene produced. |
| first_indexed | 2025-11-15T09:37:14Z |
| format | Article |
| id | upm-37450 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:37:14Z |
| publishDate | 2014 |
| publisher | John Wiley & Sons |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-374502016-04-22T08:05:30Z http://psasir.upm.edu.my/id/eprint/37450/ Engineering the lactococcal mevalonate pathway for increased sesquiterpene production Song, Adelene Ai Lian Abdullah, Janna Ong Abdullah, Mohd Puad Shafee, Norazizah Othman, Roohaida Mohd Noor, Normah Abdul Rahim, Raha Isoprenoids are a large, diverse group of secondary metabolites which has recently raised a renewed research interest due to genetic engineering advances, allowing specific isoprenoids to be produced and characterized in heterologous hosts. Many researches on metabolic engineering of heterologous hosts for increased isoprenoid production are focussed on Escherichia coli and yeasts. E. coli, as most prokaryotes, use the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway for isoprenoid production. Yeasts on the other hand, use the mevalonate pathway which is commonly found in eukaryotes. However, Lactococcus lactis is an attractive alternative host for heterologous isoprenoid production. Apart from being food-grade, this Gram-positive prokaryote uses the mevalonate pathway for isoprenoid production instead of the MEP pathway. Previous studies have shown that L. lactis is able to produce sesquiterpenes through heterologous expression of plant sesquiterpene synthases. In this work, we analysed the gene expression of the lactococcal mevalonate pathway through RT-qPCR to successfully engineer L. lactis as an efficient host for isoprenoid production. We then overexpressed the mvk gene singly or co-expressed with the mvaA gene as an attempt to increase β-sesquiphellandrene production in L. lactis. It was observed that co-expression of mvk with mvaA doubled the amount of β-sesquiphellandrene produced. John Wiley & Sons 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37450/1/37450.pdf Song, Adelene Ai Lian and Abdullah, Janna Ong and Abdullah, Mohd Puad and Shafee, Norazizah and Othman, Roohaida and Mohd Noor, Normah and Abdul Rahim, Raha (2014) Engineering the lactococcal mevalonate pathway for increased sesquiterpene production. FEMS Microbiology Letters, 355 (2). pp. 177-184. ISSN 0378-1097; ESSN: 1574-6968 http://femsle.oxfordjournals.org/content/355/2/177.abstract 10.1111/1574-6968.12469 |
| spellingShingle | Song, Adelene Ai Lian Abdullah, Janna Ong Abdullah, Mohd Puad Shafee, Norazizah Othman, Roohaida Mohd Noor, Normah Abdul Rahim, Raha Engineering the lactococcal mevalonate pathway for increased sesquiterpene production |
| title | Engineering the lactococcal mevalonate pathway for increased sesquiterpene production |
| title_full | Engineering the lactococcal mevalonate pathway for increased sesquiterpene production |
| title_fullStr | Engineering the lactococcal mevalonate pathway for increased sesquiterpene production |
| title_full_unstemmed | Engineering the lactococcal mevalonate pathway for increased sesquiterpene production |
| title_short | Engineering the lactococcal mevalonate pathway for increased sesquiterpene production |
| title_sort | engineering the lactococcal mevalonate pathway for increased sesquiterpene production |
| url | http://psasir.upm.edu.my/id/eprint/37450/ http://psasir.upm.edu.my/id/eprint/37450/ http://psasir.upm.edu.my/id/eprint/37450/ http://psasir.upm.edu.my/id/eprint/37450/1/37450.pdf |