Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis
Objectives: Large amounts of biocides are used to reduce and control bacterial growth in the healthcare sector, food production and agriculture. This work explores the effect of subinhibitory concentrations of four commonly used biocides (ethanol, hydrogen peroxide, chlorhexidine digluconate and sod...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Oxford University Press
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37315/ |
| _version_ | 1848848576396918784 |
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| author | Seier-Petersen, M. A. Jasni, A. Aarestrup, F. M. Vigre, H. Mullany, P. Roberts, A. P. Agersø, Y. |
| author_facet | Seier-Petersen, M. A. Jasni, A. Aarestrup, F. M. Vigre, H. Mullany, P. Roberts, A. P. Agersø, Y. |
| author_sort | Seier-Petersen, M. A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objectives: Large amounts of biocides are used to reduce and control bacterial growth in the healthcare sector, food production and agriculture. This work explores the effect of subinhibitory concentrations of four commonly used biocides (ethanol, hydrogen peroxide, chlorhexidine digluconate and sodium hypochlorite) on the conjugative transposition of the mobile genetic element Tn916.
Methods: Conjugation assays were carried out between Bacillus subtilis strains. The donor containing Tn916 was pre-exposed to subinhibitory concentrations of each biocide for a defined length of time, which was determined by an analysis of the transcriptional response of the promoter upstream of tet(M) using β-glucuronidase reporter assays.
Results: Ethanol significantly (P = 0.01) increased the transfer of Tn916 by 5-fold, whereas hydrogen peroxide, chlorhexidine digluconate and sodium hypochlorite did not significantly affect the transfer frequency.
Conclusions: These results suggest that exposure to subinhibitory concentrations of ethanol may induce the transfer of Tn916-like elements and any resistance genes they contain. |
| first_indexed | 2025-11-15T09:36:42Z |
| format | Article |
| id | upm-37315 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:36:42Z |
| publishDate | 2014 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-373152023-09-07T04:10:44Z http://psasir.upm.edu.my/id/eprint/37315/ Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis Seier-Petersen, M. A. Jasni, A. Aarestrup, F. M. Vigre, H. Mullany, P. Roberts, A. P. Agersø, Y. Objectives: Large amounts of biocides are used to reduce and control bacterial growth in the healthcare sector, food production and agriculture. This work explores the effect of subinhibitory concentrations of four commonly used biocides (ethanol, hydrogen peroxide, chlorhexidine digluconate and sodium hypochlorite) on the conjugative transposition of the mobile genetic element Tn916. Methods: Conjugation assays were carried out between Bacillus subtilis strains. The donor containing Tn916 was pre-exposed to subinhibitory concentrations of each biocide for a defined length of time, which was determined by an analysis of the transcriptional response of the promoter upstream of tet(M) using β-glucuronidase reporter assays. Results: Ethanol significantly (P = 0.01) increased the transfer of Tn916 by 5-fold, whereas hydrogen peroxide, chlorhexidine digluconate and sodium hypochlorite did not significantly affect the transfer frequency. Conclusions: These results suggest that exposure to subinhibitory concentrations of ethanol may induce the transfer of Tn916-like elements and any resistance genes they contain. Oxford University Press 2014 Article PeerReviewed Seier-Petersen, M. A. and Jasni, A. and Aarestrup, F. M. and Vigre, H. and Mullany, P. and Roberts, A. P. and Agersø, Y. (2014) Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis. Journal of Antimicrobial Chemotherapy, 69 (2). pp. 343-348. ISSN 0305-7453; ESSN: 1460-2091 https://academic.oup.com/jac/article/69/2/343/713830?login=true 10.1093/jac/dkt370 |
| spellingShingle | Seier-Petersen, M. A. Jasni, A. Aarestrup, F. M. Vigre, H. Mullany, P. Roberts, A. P. Agersø, Y. Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis |
| title | Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis |
| title_full | Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis |
| title_fullStr | Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis |
| title_full_unstemmed | Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis |
| title_short | Effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of Tn916 in Bacillus subtilis |
| title_sort | effect of subinhibitory concentrations of four commonly used biocides on the conjugative transfer of tn916 in bacillus subtilis |
| url | http://psasir.upm.edu.my/id/eprint/37315/ http://psasir.upm.edu.my/id/eprint/37315/ http://psasir.upm.edu.my/id/eprint/37315/ |