Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation
Haemorrhagic septicaemia (HS) is an important disease of cattle and buffaloes worldwide, caused by Pasteurella multocida type B: 2 and type E. Here, we evaluated the clinical signs, gross and histopathological changes as well as molecular detection of P. multocida in various organs of mice following...
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| Format: | Article |
| Language: | English |
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KPK Publishers
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37232/ http://psasir.upm.edu.my/id/eprint/37232/1/Effect%20of%20dose%20dependent%20oral%20inoculation%20of%20Pasteurella%20multocida%20type%20B.pdf |
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| author | Abdullah, Faez Firdaus Jesse Adamu, Lawan Abba, Yusuf Tijjani, Abdulnasir Mohammed, Konto Omar, Abdul Rahman Saharee, Abdul Aziz |
| author_facet | Abdullah, Faez Firdaus Jesse Adamu, Lawan Abba, Yusuf Tijjani, Abdulnasir Mohammed, Konto Omar, Abdul Rahman Saharee, Abdul Aziz |
| author_sort | Abdullah, Faez Firdaus Jesse |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Haemorrhagic septicaemia (HS) is an important disease of cattle and buffaloes worldwide, caused by Pasteurella multocida type B: 2 and type E. Here, we evaluated the clinical signs, gross and histopathological changes as well as molecular detection of P. multocida in various organs of mice following oral inoculation with graded doses of the bacterium. A total of 32 healthy mice were divided into four groups of 8 mice each where each group was inoculated orally with 0.4ml of P. multocida type B: 2 at the dose of 10³ (Group 1), 105 (Group 2) and 107 (Group 3) colony forming unit (CFU) respectively, while Group 4 served as a control. Post-mortem examination was conducted and gross lesions were observed. Tissue samples were collected for molecular detection and histo-pathological processing. All mice from the challenged groups showed almost no significant (P>0.05) clinical signs. Evidently, the severity of histo-pathological lesions in various organs was significantly high in groups 2 and 3. Through polymerase chain reaction detection (PCR), P. mulltocida type B: 2 was detected in all the experimental groups. Overall, the oral inoculation of mice at different doses of P. multocida type B: 2 results in clinical signs and histopathological lesions of haemorrhagic septicaemia infection. |
| first_indexed | 2025-11-15T09:36:20Z |
| format | Article |
| id | upm-37232 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:36:20Z |
| publishDate | 2014 |
| publisher | KPK Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-372322015-08-27T07:31:40Z http://psasir.upm.edu.my/id/eprint/37232/ Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation Abdullah, Faez Firdaus Jesse Adamu, Lawan Abba, Yusuf Tijjani, Abdulnasir Mohammed, Konto Omar, Abdul Rahman Saharee, Abdul Aziz Haemorrhagic septicaemia (HS) is an important disease of cattle and buffaloes worldwide, caused by Pasteurella multocida type B: 2 and type E. Here, we evaluated the clinical signs, gross and histopathological changes as well as molecular detection of P. multocida in various organs of mice following oral inoculation with graded doses of the bacterium. A total of 32 healthy mice were divided into four groups of 8 mice each where each group was inoculated orally with 0.4ml of P. multocida type B: 2 at the dose of 10³ (Group 1), 105 (Group 2) and 107 (Group 3) colony forming unit (CFU) respectively, while Group 4 served as a control. Post-mortem examination was conducted and gross lesions were observed. Tissue samples were collected for molecular detection and histo-pathological processing. All mice from the challenged groups showed almost no significant (P>0.05) clinical signs. Evidently, the severity of histo-pathological lesions in various organs was significantly high in groups 2 and 3. Through polymerase chain reaction detection (PCR), P. mulltocida type B: 2 was detected in all the experimental groups. Overall, the oral inoculation of mice at different doses of P. multocida type B: 2 results in clinical signs and histopathological lesions of haemorrhagic septicaemia infection. KPK Publishers 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37232/1/Effect%20of%20dose%20dependent%20oral%20inoculation%20of%20Pasteurella%20multocida%20type%20B.pdf Abdullah, Faez Firdaus Jesse and Adamu, Lawan and Abba, Yusuf and Tijjani, Abdulnasir and Mohammed, Konto and Omar, Abdul Rahman and Saharee, Abdul Aziz (2014) Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation. Research Opinions in Animal & Veterinary Sciences, 4 (10). pp. 535-539. ISSN 2221-1896; ESSN: 2223-0343 http://www.roavs.com/archive/vol-4-issue-10-2014.htm |
| spellingShingle | Abdullah, Faez Firdaus Jesse Adamu, Lawan Abba, Yusuf Tijjani, Abdulnasir Mohammed, Konto Omar, Abdul Rahman Saharee, Abdul Aziz Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation |
| title | Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation |
| title_full | Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation |
| title_fullStr | Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation |
| title_full_unstemmed | Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation |
| title_short | Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation |
| title_sort | effect of dose dependent oral inoculation of pasteurella multocida type b: 2 in mice: molecular detection and histopathological evaluation |
| url | http://psasir.upm.edu.my/id/eprint/37232/ http://psasir.upm.edu.my/id/eprint/37232/ http://psasir.upm.edu.my/id/eprint/37232/1/Effect%20of%20dose%20dependent%20oral%20inoculation%20of%20Pasteurella%20multocida%20type%20B.pdf |