Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols
Recent studies have demonstrated the ability of midterm human amniotic fluid cells to generate dopaminergic (DA) neurons where none using amniotic fluid cells from full term pregnancies has been published. We have established rat AFS cell lines from full term pregnancies, where characteristics of...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Published: |
Tissue Engineering and Regenerative Medicine Society of Malaysia
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/37108/ |
| _version_ | 1848848519860846592 |
|---|---|
| author | Hamzah, Siti Nurusaadah Vidyadaran, Sharmili Nordin, Norshariza |
| author_facet | Hamzah, Siti Nurusaadah Vidyadaran, Sharmili Nordin, Norshariza |
| author_sort | Hamzah, Siti Nurusaadah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Recent studies have demonstrated the ability of midterm human amniotic fluid cells to
generate dopaminergic (DA) neurons where none using amniotic fluid cells from full term
pregnancies has been published. We have established rat AFS cell lines from full term
pregnancies, where characteristics of highly potent cells were observed with their ability to
differentiate into derivatives representing the three primary germ layers including neural cells.
Here we aimed to explore the differentiation potential of our newly established rat full term
AFS cells to differentiate into DA neurons phenotype in vitro. Neural-like cells expressing
mature and DA neurons phenotype were successfully derived using two established protocols
of adherent and non-adherent neural induction assays. These findings have provided us an
insight into the use of full term AFS cells as a valuable tool in cell replacement therapy for
neurodegenerative diseases such as Parkinson’s disease (PD) |
| first_indexed | 2025-11-15T09:35:48Z |
| format | Article |
| id | upm-37108 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:35:48Z |
| publishDate | 2014 |
| publisher | Tissue Engineering and Regenerative Medicine Society of Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-371082016-01-14T07:57:26Z http://psasir.upm.edu.my/id/eprint/37108/ Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols Hamzah, Siti Nurusaadah Vidyadaran, Sharmili Nordin, Norshariza Recent studies have demonstrated the ability of midterm human amniotic fluid cells to generate dopaminergic (DA) neurons where none using amniotic fluid cells from full term pregnancies has been published. We have established rat AFS cell lines from full term pregnancies, where characteristics of highly potent cells were observed with their ability to differentiate into derivatives representing the three primary germ layers including neural cells. Here we aimed to explore the differentiation potential of our newly established rat full term AFS cells to differentiate into DA neurons phenotype in vitro. Neural-like cells expressing mature and DA neurons phenotype were successfully derived using two established protocols of adherent and non-adherent neural induction assays. These findings have provided us an insight into the use of full term AFS cells as a valuable tool in cell replacement therapy for neurodegenerative diseases such as Parkinson’s disease (PD) Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article PeerReviewed Hamzah, Siti Nurusaadah and Vidyadaran, Sharmili and Nordin, Norshariza (2014) Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols. Regenerative Research, 3 (2). pp. 140-141. ISSN 2232-0822 http://regres.tesma.org.my/index-8.html |
| spellingShingle | Hamzah, Siti Nurusaadah Vidyadaran, Sharmili Nordin, Norshariza Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols |
| title | Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols |
| title_full | Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols |
| title_fullStr | Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols |
| title_full_unstemmed | Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols |
| title_short | Differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols |
| title_sort | differentiation of rat full term amniotic fluid stem cells into dopaminergic neurons phenotype via adherent and non-adherent neural induction protocols |
| url | http://psasir.upm.edu.my/id/eprint/37108/ http://psasir.upm.edu.my/id/eprint/37108/ |