Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals

QR Code

Development of a bacterial-based tetrazolium dye (MTT) assay for monitoring of heavy metals

An inhibitive assay for metals using a bacterial respiratory assay system is presented. The assay is based on the ability of bacteria to reduce the water soluble tetrazolium dye (MTT). The isolate was tentatively identified as Bacillus sp. strain Khayat. The Bacillus sp based MTT assay is sensitive...

Full description

Bibliographic Details
Main Authors:	Wasoh @ Mohamad Isa, Helmi, Wan Johari, Wan Lutfi, Syahir, A., Abd. Shukor, Mohd Yunus, Abd Aziz, Nor Azwady, Shaharuddin, N. A., Mustafa, Muskhazli
Format:	Article
Language:	English
Published:	Friends Science Publishers 2014
Online Access:	http://psasir.upm.edu.my/id/eprint/37052/ http://psasir.upm.edu.my/id/eprint/37052/1/Development%20of%20a%20bacterial.pdf

Similar Items

MTT assay technique
by: Ashour, Abdelkader Elbadawy Abbas
Published: (2018)

Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals
by: Gunasekaran, Baskaran, et al.
Published: (2018)

Development of a yeast inhibitive assay for anionic heavy metals using the 1-(4,5-Dimethylthiazol-2-Yl)-3,5-Diphenyltetrazolium Bromide (MTT) assay
by: Mohd Sadli, Farah Najieha, et al.
Published: (2022)

Drugs susceptibility testing of mycobacterium tuberculosis using direct tetrazolium microplate assay (TEMA)
by: Yahaya, Mohammad Lukman
Published: (2015)

Cytotoxicity study of Basella alba leaves extract on brine shrimp lethality bioassay and mtt assay
by: Azad, Abul Kalam, et al.
Published: (2019)

Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri
by: Sabullah, Mohd Khalizan, et al.
Published: (2015)

A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman
by: Abdul Rahman, Nur Fatihah
Published: (2015)

Viability test on Hevea seeds by the Tetrazolium method
by: Mohamad Husin, Sakhibun, et al.
Published: (1981)

Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor
by: Mohd Noor, Nurain Zulaika
Published: (2015)

Assay for heavy metals.
by: Abd. Shukor, Mohd. Yunus, et al.
Published: (2010)

Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Pangasius hypophthalmus (Sauvage, 1878)
by: Aidil, Mohd S., et al.
Published: (2013)

Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus
by: Zulkifli, Ain Farihah, et al.
Published: (2017)

Comparison of Microtox and Xenoassay light as a near real time river monitoring assay for heavy metals
by: Halmi, Mohd Izuan Effendi, et al.
Published: (2014)

Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells
by: Lim, Su Wen, et al.
Published: (2015)

Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP
by: Abdul Habib, Nur Muhamad Syahir, et al.
Published: (2017)

Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus
by: Muhammad, Umar Abubakar, et al.
Published: (2016)

Biodecolorization of azo dyes by microorganisms isolated from Serdang and Merambong soils
by: Wan Johari, Wan Lutfi, et al.
Published: (2014)

Decolorization of azo dyes by local microorganisms
by: Wan Johari, Wan Lutfi, et al.
Published: (2013)

Yeast inhibitive assay for anionic heavy metals: a review
by: Mohd Sadli, Farah Najieha, et al.
Published: (2022)

Biosorption of Azo Dyes by bacterial biomass: a review
by: Abubakar, Abdusssamad, et al.
Published: (2023)

Effects of heavy metals on bacterial growth parameters in degradation of phenol by an Antarctic bacterial consortium
by: Tengku-Mazuki, Tengku Athirrah, et al.
Published: (2023)

A rapid inhibitive enzyme assay for monitoring heavy metals pollution in the Juru industrial estate
by: Halmi, Mohd Izuan Effendi, et al.
Published: (2015)

Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE
by: Halmi, Mohd Izuan Effendi, et al.
Published: (2019)

Efficiency of polycyclic aromatic hydrocarbons (PAHs) degrading consortium in resisting heavy metals during PAHs degradation
by: Umar, Zubairu Darma, et al.
Published: (2018)

Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia
by: Wakid, Sarini Ahmad, et al.
Published: (2024)

Decolorization of metanil yellow dye by free and immobilized bacterial cells
by: Muliadi, Fatin Natasha Amira
Published: (2021)

Effect of heavy metals to bacterial culture and the removal of heavy metals from an industrial effluent
by: Sannasi, P., et al.
Published: (2010)

Peatland fungi: identification, application in dye decolourization and bacterial inactivation in greywater
by: Mohammed, Efaq Ali Noman
Published: (2021)

Development of an inhibitive enzyme assay for copper
by: Abd. Shukor, Mohd. Yunus, et al.
Published: (2009)

Biosssay for heavy metals.
by: Abd. Shukor, Mohd. Yunus
Published: (2004)

Enzyme assay for metal-reducing enzyme
by: Abd. Shukor, Mohd. Yunus, et al.
Published: (2009)

The growth potential and bioaccumulation ability of probiotics under the exposure of different heavy metals
by: Abang Mohamad Moasili, Abang Mohamad Musawi, et al.
Published: (2019)

Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix
by: Nordin, Noreen, et al.
Published: (2023)

Nickel heavy metal and dye removal using polyethersulfone microcapsule
by: Lee, Yu Sin
Published: (2024)

A novel characterization of alginate-attapulgite-calcium carbonate (AAC) gel adsorption in bacterial biodegradation of used engine oil (UEO)
by: Elhamrouni, Izeddin Abdalla, et al.
Published: (2023)

Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration
by: Wasoh @ Mohamad Isa, Helmi
Published: (2009)

Optimization and maximization of hexavalent molybdenum reduction to Mo-blue by Serratia sp. strain MIE2 using response surface methodology
by: Halmi, Mohd Izuan Effendi, et al.
Published: (2016)

Physical, chemical, and biological methods for the removal of arsenic compounds
by: Lim, King Ting, et al.
Published: (2014)

Determination of the mutagenic potential of commonly marketed Ghanaian medicinal plant formulations using the bacterial reverse mutation assay
by: Senayah, Fidelia
Published: (2015)

Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections
by: Mukap, M., et al.
Published: (2018)