Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens
Fowl adenovirus (FAdV) is distributed worldwide and causes economic losses in the poultry industry. The objectives of this study were to determine the hexon and fiber gene changes in an attenuated FAdV isolate from Malaysia in specific pathogen-free chicken embryonated eggs (SPF CEE) and its infecti...
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| Format: | Article |
| Language: | English |
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The Korean Society of Veterinary Science
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37025/ http://psasir.upm.edu.my/id/eprint/37025/1/Hexon%20and%20fiber%20gene%20changes%20in%20an%20attenuated%20fowl%20adenovirus%20isolate%20from%20Malaysia%20in%20embryonated%20chicken%20eggs%20and%20its%20infectivity%20in%20chickens.pdf |
| _version_ | 1848848497163370496 |
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| author | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza |
| author_facet | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza |
| author_sort | Mohamed Sohaimi, Norfitriah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Fowl adenovirus (FAdV) is distributed worldwide and causes economic losses in the poultry industry. The objectives of this study were to determine the hexon and fiber gene changes in an attenuated FAdV isolate from Malaysia in specific pathogen-free chicken embryonated eggs (SPF CEE) and its infectivity in commercial broiler chickens. SPF CEE were inoculated with 0.1 mL FAdV inoculum via the chorioallantoic membrane (CAM) for 20 consecutive passages. The isolate at passage 20 (E20), with a virus titer of 108.7TCID50/mL (TCID50, 50% tissue culture infective dose), was inoculated (0.5 mL) into one-day-old commercial broiler chicks either via oral or intraperitoneal routes. The study demonstrated that 100% embryonic mortality was recorded from E2 to E20 with a delayed pattern at E17 onwards. The lesions were confined to the liver and CAM. Substitutions of amino acids in the L1 loop of hexon at positions 49 and 66, and in the knob of fiber at positions 318 and 322 were recorded in the E20 isolate. The isolate belongs to serotype 8b and is non-pathogenic to broiler chickens, but it is able to induce a FAdV antibody titer. It appears that molecular changes in the L1 loop of hexon and the knob of fiber are markers for FAdV infectivity. |
| first_indexed | 2025-11-15T09:35:26Z |
| format | Article |
| id | upm-37025 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:35:26Z |
| publishDate | 2018 |
| publisher | The Korean Society of Veterinary Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-370252020-07-06T03:14:25Z http://psasir.upm.edu.my/id/eprint/37025/ Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza Fowl adenovirus (FAdV) is distributed worldwide and causes economic losses in the poultry industry. The objectives of this study were to determine the hexon and fiber gene changes in an attenuated FAdV isolate from Malaysia in specific pathogen-free chicken embryonated eggs (SPF CEE) and its infectivity in commercial broiler chickens. SPF CEE were inoculated with 0.1 mL FAdV inoculum via the chorioallantoic membrane (CAM) for 20 consecutive passages. The isolate at passage 20 (E20), with a virus titer of 108.7TCID50/mL (TCID50, 50% tissue culture infective dose), was inoculated (0.5 mL) into one-day-old commercial broiler chicks either via oral or intraperitoneal routes. The study demonstrated that 100% embryonic mortality was recorded from E2 to E20 with a delayed pattern at E17 onwards. The lesions were confined to the liver and CAM. Substitutions of amino acids in the L1 loop of hexon at positions 49 and 66, and in the knob of fiber at positions 318 and 322 were recorded in the E20 isolate. The isolate belongs to serotype 8b and is non-pathogenic to broiler chickens, but it is able to induce a FAdV antibody titer. It appears that molecular changes in the L1 loop of hexon and the knob of fiber are markers for FAdV infectivity. The Korean Society of Veterinary Science 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/37025/1/Hexon%20and%20fiber%20gene%20changes%20in%20an%20attenuated%20fowl%20adenovirus%20isolate%20from%20Malaysia%20in%20embryonated%20chicken%20eggs%20and%20its%20infectivity%20in%20chickens.pdf Mohamed Sohaimi, Norfitriah and Bejo, Mohd Hair and Omar, Abdul Rahman and Ideris, Aini and Mat Isa, Nurulfiza (2018) Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens. Journal of Veterinary Science, 19 (6). pp. 759-770. ISSN 1229-845X; ESSN: 1976-555X https://vetsci.org/search.php?where=aview&id=10.4142/jvs.2018.19.6.759&code=0118JVS&vmode=AONLY 10.4142/jvs.2018.19.6.759 |
| spellingShingle | Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens |
| title | Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens |
| title_full | Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens |
| title_fullStr | Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens |
| title_full_unstemmed | Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens |
| title_short | Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens |
| title_sort | hexon and fiber gene changes in an attenuated fowl adenovirus isolate from malaysia in embryonated chicken eggs and its infectivity in chickens |
| url | http://psasir.upm.edu.my/id/eprint/37025/ http://psasir.upm.edu.my/id/eprint/37025/ http://psasir.upm.edu.my/id/eprint/37025/ http://psasir.upm.edu.my/id/eprint/37025/1/Hexon%20and%20fiber%20gene%20changes%20in%20an%20attenuated%20fowl%20adenovirus%20isolate%20from%20Malaysia%20in%20embryonated%20chicken%20eggs%20and%20its%20infectivity%20in%20chickens.pdf |