Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique

Effluents from municipal and industrial wastewater treatment plants, agricultural run-off and domestic drainage add numerous pollutants to the aquatic system. Among them is 17β-estradiol (E2), which is known as one of the strongest estrogenic chemical in the environment and commonly found in wastewa...

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Main Authors: Ismail, Ahmad, Hazizan, Akhlily Farhana, Zulkifli, Syaizwan Zahmir, Mohamat Yusuff, Ferdaus, Omar, Hishamuddin, Arizono, Koji
Format: Article
Language:English
Published: Marsland Press 2014
Online Access:http://psasir.upm.edu.my/id/eprint/37021/
http://psasir.upm.edu.my/id/eprint/37021/1/Determination%20of%2017%CE%B2.pdf
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author Ismail, Ahmad
Hazizan, Akhlily Farhana
Zulkifli, Syaizwan Zahmir
Mohamat Yusuff, Ferdaus
Omar, Hishamuddin
Arizono, Koji
author_facet Ismail, Ahmad
Hazizan, Akhlily Farhana
Zulkifli, Syaizwan Zahmir
Mohamat Yusuff, Ferdaus
Omar, Hishamuddin
Arizono, Koji
author_sort Ismail, Ahmad
building UPM Institutional Repository
collection Online Access
description Effluents from municipal and industrial wastewater treatment plants, agricultural run-off and domestic drainage add numerous pollutants to the aquatic system. Among them is 17β-estradiol (E2), which is known as one of the strongest estrogenic chemical in the environment and commonly found in wastewater. This study was conducted to assess present concentration of E2 in aquatic environment of Peninsular Malaysia. Water samples were collected from rivers, estuaries and lakes at the northern, central and southern regions of Peninsular Malaysia. E2 concentration was determined using ELISA technique. Results revealed that the center region has the highest E2 average concentration (149.19 ng/L), followed by the northern region (95.04 ng/L) and southern region (15.66 ng/L). These results exhibit size of human population and activities may directly related with E2 elevation in aquatic environment as reflected by high human population in the central region. The lakes have the lowest concentration (11.83 ng/L), thus suggesting E2 discharges flow directly into river system. Comparison with other countries demonstrates E2 in aquatic environment in Peninsular Malaysia is higher several folds. If no immediate actions are implemented to control E2 level in the environment, there could be some adverse effects, especially to male aquatic animals, such as ova-testis, feminization, fertility reduction and villetogenin gene expression. Application of suitable bio-indicator is recommended in order to monitor estrogenic activities in aquatic environment.
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spelling upm-370212018-07-25T03:45:06Z http://psasir.upm.edu.my/id/eprint/37021/ Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique Ismail, Ahmad Hazizan, Akhlily Farhana Zulkifli, Syaizwan Zahmir Mohamat Yusuff, Ferdaus Omar, Hishamuddin Arizono, Koji Effluents from municipal and industrial wastewater treatment plants, agricultural run-off and domestic drainage add numerous pollutants to the aquatic system. Among them is 17β-estradiol (E2), which is known as one of the strongest estrogenic chemical in the environment and commonly found in wastewater. This study was conducted to assess present concentration of E2 in aquatic environment of Peninsular Malaysia. Water samples were collected from rivers, estuaries and lakes at the northern, central and southern regions of Peninsular Malaysia. E2 concentration was determined using ELISA technique. Results revealed that the center region has the highest E2 average concentration (149.19 ng/L), followed by the northern region (95.04 ng/L) and southern region (15.66 ng/L). These results exhibit size of human population and activities may directly related with E2 elevation in aquatic environment as reflected by high human population in the central region. The lakes have the lowest concentration (11.83 ng/L), thus suggesting E2 discharges flow directly into river system. Comparison with other countries demonstrates E2 in aquatic environment in Peninsular Malaysia is higher several folds. If no immediate actions are implemented to control E2 level in the environment, there could be some adverse effects, especially to male aquatic animals, such as ova-testis, feminization, fertility reduction and villetogenin gene expression. Application of suitable bio-indicator is recommended in order to monitor estrogenic activities in aquatic environment. Marsland Press 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37021/1/Determination%20of%2017%CE%B2.pdf Ismail, Ahmad and Hazizan, Akhlily Farhana and Zulkifli, Syaizwan Zahmir and Mohamat Yusuff, Ferdaus and Omar, Hishamuddin and Arizono, Koji (2014) Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique. Life Science Journal, 11 (8). pp. 673-679. ISSN 1097-8135; ESSN: 2372-613X http://www.lifesciencesite.com/lsj/life1108/ 10.7537/marslsj110814.97
spellingShingle Ismail, Ahmad
Hazizan, Akhlily Farhana
Zulkifli, Syaizwan Zahmir
Mohamat Yusuff, Ferdaus
Omar, Hishamuddin
Arizono, Koji
Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique
title Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique
title_full Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique
title_fullStr Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique
title_full_unstemmed Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique
title_short Determination of 17β-estradiol concentration in aquatic environment of Peninsular Malaysia using the ELISA technique
title_sort determination of 17β-estradiol concentration in aquatic environment of peninsular malaysia using the elisa technique
url http://psasir.upm.edu.my/id/eprint/37021/
http://psasir.upm.edu.my/id/eprint/37021/
http://psasir.upm.edu.my/id/eprint/37021/
http://psasir.upm.edu.my/id/eprint/37021/1/Determination%20of%2017%CE%B2.pdf